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DETERMINATION OF H/D ISOTOPIC RATIO AT NATURAL ABUNDANCE LEVEL IN WINE BY QUANTITATIVE NMR SPECTROMETRY

Objective


The use of nuclear magnetic resonance (NMR) to detect the fraudulent addition of sugar and water to grapes during wine making, to increase the alcoholic strength of wine is well established. The deuterium isotope redistribution from sugar to ethanol during fermentation is characterized by the hydrogen to deuterium H/D ratio but the method requires a set of reference ethanols of known H/D ratio at natural abundance levels for the calibration, and alignment of NMR spectrometers. In the project 3 ethanol reference materials (RM) from different phytobiological origins were certified: ethanol from sugar beet, a Calvin type plant which normally has a low deuterium content; ethanol from grape having a medium deuterium content; ethanol from maize, a Hatch-Slack type plant with high deuterium content.

A reference tetramethylurea (TMU) sample for use as an internal standard was also prepared in 500 and 1000 ml quantities for official site specific natural isotope fractionation (SNIF) NMR measurements of wine and must.
The use of Nuclear Magnetic Resonance (NMR) to detect the fraudulent addition of sugar and water to grapes during wine making, to increase the alcoholic strength of wine is well established.
The deuterium isotope redistribution from sugar to ethanol during fermentation is characterized by the H/D NMR but the method requires a set of reference ethanols of known H/D ratio at natural abundance levels for the calibration and alignment of NMR spectrometers. The objective of the project is to prepare three ethanol reference materials from different phytobiological origins:
- ethanol from sugar beet, a Calvin type plant which normally has a low deuterium content;
- ethanol from grape having a medium deuterium content;
- ethanol from maize, a Hatch-Slack type plant with high deuterium content.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Université de Nantes
Address
2 Rue De La Houssinière
44072 Nantes
France

Participants (14)

Chemisches Untersuchungsamt Speyer
Germany
Address
Nikolaus Von Weis-straße 1
67346 Speyer
Eurofins SA
France
Address
Site De La Géraudière
44073 Nantes
Istituto Agrario Provinciale di San Michele all'Adige
Italy
Address
Via Mach 1
38010 San Michele All'adige (Trento)
Kodak Ltd
United Kingdom
Address
Headstone Drive
HA1 1PF Harrow
Laboratoire Interregional de la Répression des Fraudes
France
Address
2 Rue Saint Pierre
34024 Montpellier
Laboratory of the Government Chemist (LGC)
United Kingdom
Address
Queens Road
TW11 0LY Teddington
Pernod Ricard SA
France
Address
120 Avenue Du Maréchal Foch
94015 Créteil
Philipps-Universität Marburg
Germany
Address
Hans Meerwein Straße
35043 Marburg
TNO-CIVO Institutes in Zeist
Netherlands
Address
P.o. Box 108
3700 Ac Zeist
University College of Dublin
Ireland
Address
Belfield
4 Dublin
University of Copenhagen
Denmark
Address
5,Universitetsparken
2100 Copenhagen 0
Università degli Studi di Bologna
Italy
Address

40100 Bologna
Universität Regensburg NWF II
Germany
Address

93053 Regensburg
Université de Strasbourg I (Université Louis Pasteur)
France
Address
74 Route Du Rhin
67401 Illkirch