Skip to main content

AFLATOXIN B1 IN COMPOUND ANIMAL FEED (CRMs and Collaborative Study of Proposed Community Method)

Objective


Animal feed is subject to control in accordance with Directive 86/229/EEC which prescribes maximum levels for aflatoxin B1 in finished feed of 50, 30, and 10 ug/kg depending on the animals to be fed. Methods were not sufficiently sensitive to allow reliable quantitation of aflatoxin B1 at 10 ug/kg and were vulnerable to interference by other feed components, notably citrus pulp and manioc. The objective of the project was to provide reference materials of compound animal feed with an aflatoxin content certified at around 10 ug/kg, and to organise a study of a proposed Community method for determination of the toxin in compound feeds.

A preliminary intercomparison study showed that good agreement could be obtained with methods modified to eliminate interference by manioc and citrus pulp at a level of 15 ug/kg. Good results were also obtained by immunoassay methods (test kits and immunoaffinity columns). A candidate Community method based on high performance liquid chromatography (HPLC) was successfully collaboratively tested by 25 laboratories. Compound feed reference materials (RM) containing aflatoxin B1 at around 1 and 10 ug/kg have been prepared and shown to be homogenous and stable.
Animal feed is subject to control in accordance with Directive 86/229/EEC which prescribes maximum levels for aflatoxin B1 in finished feed of 50, 30 and 10 ug/kg depending on the animals to be fed.
The current legal method is not sufficiently sensitive to allow reliable quantitation of aflatoxin B1 at 10 ug/kg and is vulnerable to interference by other feed components, notably citrus pulp and manioc.
The objective of the project is:
a) to provide reference materials of compound animal feed with an aflatoxin content certified at around 10 ug/kg,
b) to organise a study of a proposed Community method for determination of the toxin in compound feeds.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Rijksinstituut voor Volksgezondheid en Milieuhygiene (R.I.V.M)
Address

3720 BA Bilthoven
Netherlands

Participants (16)

Hendriks Voeders B.V.
Netherlands
Address
38,Veerstraat
5830 MA Bocmeer
Istituto Superiore della Sanità
Italy
Address
Viale Regina Elena 299
00161 Roma
Laboratorio Nacional de Engenharia e Technologia Industrial
Portugal
Address
1,Rua Vale Formoso
1900 Lisboa
Laboratory of the Government Chemist (LGC)
United Kingdom
Address
Queens Road
TW11 0LY Teddington
Landwirtschaftliche Untersuchungs- und Forschungsanstalt
Germany
Address
Gutenbergstraße 75-77
24116 Kiel
MAFF Food Science Laboratory
United Kingdom
Address
Norwich Research Park Colney
NR4 7UQ Norwich
Max-von-Pettenkofer-Institut
Germany
Address
Thielallee 88-92
14195 Berlin
Milchwirtschaftliche Untersuchungs- und Versuchsanstalt Kempten
Germany
Address
Hirnbeinstraße 10
87435 Kempten
Ministerio de Sanidad y Consumo - Instituto de Salud Carlos III Carretera Majadahonda
Spain
Address
Pozuelo, Km 2,2
28220 Majadahonda (Madrid)
Ministère de l'Economie, des Finances et du Budget
France
Address
26 Avenue Du Coëtlogon
35000 Rennes
RIKILT
Netherlands
Address

6700 AE Wageningen
Rank Hovis McDougall Technology Ltd
United Kingdom
Address
Lord Rank Centre Lincoln Road
HP12 3QR High Wycombe
Rijksuniversiteit van Gent
Belgium
Address
72,Harelbekestraat
9000 Gent
Royal Veterinary and Agricultural University
Denmark
Address
12,Bülowsvej
1870 Copenhagen V
State Laboratory
Ireland
Address
Abbotstown - Castleknock
15 Dublin
TNO Biotechnology & Chemistry Institute
Netherlands
Address

3700 AJ Zeist