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DEVELOPMENT OF A METHOD FOR THE DETERMINATION OF DRY GLUTEN IN DURUM WHEAT

Objective


A method for determination of dry gluten in durum wheat has been tested in 9 laboratories using different durum products: a wholemeal obtained by Bipea and distributed to all the participants, and the wholemeal obtained by each participant using its own mill. Three levels of protein content were controlled.
Repeatability and reproducibility, in the range 9-10% of dry gluten, were respectively about 5% and 20%.
These results should be considered provisional, because all the participants do not use the same methods, particularly for the washing phase. This is not surprising, however, because that step is difficult to standardize.
The good correlation between the quantity of wet and dry gluten obtained with the two sorts of products seems to minimize the milling effect. Nevertheless, complementary investigations must be done, after a technician training session, to complete the study especially for reproducibility.
The quality of durum wheat is determined by measuring its gluten and protein content. Limits of these two parameters are given by the EEC Regulation on durum wheat quality (EEC 2731 modified by 2094/87).

Protein is measured by NIR or Kjeldahl methods but there is no agreed method for the gluten determination in durum wheat.

The Council asked the Commission to propose an agreed method. A procedure suggested by the Italian pasta industry will be subject of a collaborative study involving the main laboratories of the Member States working in this field.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

BIPEA
Address
6/14,Avenue Louis Roche
92230 Gennevilliers
France

Participants (9)

Barilla - Laboratori Centrali
Italy
Address
Via Mantova 166
43100 Parma
Bundesforschungsanstalt für Getreide- und Kartoffelverarbeitung
Germany
Address
Schützenberg 12
32756 Detmold
Cereals Institute
Greece
Address
P.o. Box 14514
54110 Thessaloniki
Flour Milling and Baking Research Association (FMBRA)
United Kingdom
Address
Chorleywood
WD3 5SH Rickmansworth
Istituto Sperimentale per la Cerealicoltura
Italy
Address
Via Cassia 176
00191 Roma
Ministère des Affaires Economiques - Laboratoire Central
Belgium
Address
17A,rue De La Senne
1000 Bruxelles
PANZANI SA
France
Address
131 Avenue Corot
13013 Marseille
SENPA - Servicio Nacional de Productos Agrarios
Spain
Address
8,C/beneficencia
28004 Madrid
Société Coopérative Agricole de Romilly Mery
France
Address
23 Avenue Du Château
10101 Romilly-sur-seine