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NITRATE DETERMINATION IN FOODSTUFFS

Objective


The objective of the project was to improve methodology for nitrate determination in foodstuffs through intercomparison and to prepare certified reference materials (CRM). A first intercomparison on orange juice materials (blank and spiked) and beetroot juice involving high performance liquid chromatography (HPLC) techniques, cadmium reduction methods and enzymatic kits have shown the differences between methods were not significant, the relative coefficient of variation being less than 10%. A reference material consisting of freeze dried lettuce powder is being prepared.
The level of nitrate in food is increasingly controlled because of possible risk to human health and especially to children. There is controversy over the possible relationship between nitrate and formation of carcinogenic nitrosamines. For baby food, the legal limit in several Member States is 50 mg/kg, by comparison, the EC legal limit for fresh water is 40 mg/kg.
Nitrate level can reach 3 to 4 g/kg in fresh vegetable.
The objective of the project is to improve methodology through intercomparison and to prepare CRMs if necessary.

Coordinator

Horticulture Research International
Address
Wellesbourne
CV35 9EF Warwick
United Kingdom

Participants (22)

Bundesforschungsanstalt
Germany
Address
Rüdeshelmer Straße 12-14
65366 Geisenheim
Campden and Chorleywood Food Research Association (CCFRA)
United Kingdom
Address
Station Road
GL55 6LD Chipping Campden
Conselleria de Sanidad
Spain
Address
2,Amadeo De Saboya
46004 Valencia
Danish Research Service for Plant & Soil Science
Denmark
Address
12,Kirstinebjergvej
5792 Arslev
Deutsche Granini GmbH & Co KG
Germany
Address
Kammerratsheide
33609 Bielefeld
Eden-Waren GmbH
Germany
Address

6232 Bad Soden
Ente Ricerca Applicata
Italy
Address
Via Juri Gagarin 15
06074 Ellera Umbra Perugia
HENGSTENBERG
Germany
Address
Mettinger Straße 109
73728 Esslingen Am Neckar
Institut d'Hygiène et d'Epidémiologie
Belgium
Address
14,Rue J. Wytsman
1050 Bruxelles
Istituto Superiore della Sanità
Italy
Address
Viale Regina Elena 299
00161 Roma
Laboratoire Interrégional de la Répression des Fraudes
France
Address
25 Avenue De La République
91305 Massy
Laboratoires Associés de Recherches Agricoles
France
Address
75 Voie Du Toec
31300 Toulouse
Laboratorio Greenlab
Italy
Address
Via Malpighi 7
47100 Forlí
Laboratory of the Government Chemist (LGC)
United Kingdom
Address
Queen's Road
TW11 0LY Teddington
Landesuntersuchungsamt für das Gesundheitswesen Nordbayern
Germany
Address
Theaterstraße 23
97070 Würzburg
National Food Agency of Denmark
Denmark
Address
19,Mörkhöj Bygade
2860 Söborg
Rijksuniversiteit van Gent
Belgium
Address
72,Harelbekestraat
9000 Gent
STAZIONE SPERIMENTALE PER L'INDUSTRIA DELLE CONSERVE ALIMENTARI
Italy
Address
Viale Faustino Tanara 31/A
Parma
Servicio Vasco de Salud
Spain
Address
60,Maria Diaz De Haro
48010 Bilbao
Station Fédérale de Recherches en Chimie Agricole, Hygiène et Environ.
Switzerland
Address

3097 Liebefeld
TEAGASC - National Food Centre
Ireland
Address
Dunsinea Castleknock
15 Dublin
TNO Biotechnology & Chemistry Institute
Netherlands
Address

3700 AJ Zeist