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IMPROVEMENTS IN THE DETERMINATION OF FOLATES IN FOOD

Objective


Comparisons of the performance of the different types of method for analysis of folate in food has been carried out with a view to identifying improved and more specific procedures. This showed the need to improve extraction and deconjugation procedures, calibration and identification of folate forms by high performance liquid chromatography (HPLC) and calibration in the PBA/RIA (protein binding assay/radioimmunoassay) methods.
The current project is undertaken as a consequence of an intercomparison made under project no 341 which identified serious difficulties in the analysis of folate in food.

The objective is to examine through intercomparisons the performance of the different types of method with a view to identifying improved and more specific procedures for folate possibly based on HPLC or immunoassays which can offer potentially greater sensitivity. The folate content of several food RMs will be certified if it is feasible.

Topic(s)

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Call for proposal

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Coordinator

Laboratory of the Government Chemist (LGC)
Address
Queen's Road
TW11 0LY Teddington
United Kingdom

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Participants (14)

Aston University
United Kingdom
Address
Aston Triangle
B4 7ET Birmingham

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CSIC - Instituto del Frio
Spain
Address
Ciudad Universitaria
28040 Madrid

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Federal Research Centre for Nutrition
Germany
Address
Haid-und-neustr.9
76131 Karlsruhe

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Justus-Liebig-Universität Giessen
Germany
Address
Wilhelmstraße 20
35398 Giessen

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National Food Administration
Sweden
Address
5,hamnesplanaden
75126 Uppsala

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National Food Agency of Denmark
Denmark
Address
19,mörkhöj Bygade
2860 Söborg

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Steins Laboratorium
Denmark
Address
8,hjaltesvej
7500 Holstebro

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TNO Toxicology and Nutrition Institute
Netherlands
Address

3700 AJ Zeist

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UNIVERSITAET - GESAMTHOCHSCHULE PADERBORN
Germany
Address
Warburger Strasse 100
33098 Paderborn

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Unilever UK Central Resources Ltd
United Kingdom
Address
Colworth House
MK44 1LQ Sharnbrook

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University College of Cork
Ireland
Address

Cork

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University of Lund and Institute of Technology
Sweden
Address

22100 Lund

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University of Lund and Institute of Technology
Sweden
Address

22100 Lund

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Universität Basel
Switzerland
Address
Vesalianum, Vesalgasse 1
4051 Basel

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