IMPROVEMENTS IN THE DETERMINATION OF FOLATES IN FOOD

Objective

Comparisons of the performance of the different types of method for analysis of folate in food has been carried out with a view to identifying improved and more specific procedures. This showed the need to improve extraction and deconjugation procedures, calibration and identification of folate forms by high performance liquid chromatography (HPLC) and calibration in the PBA/RIA (protein binding assay/radioimmunoassay) methods.
The current project is undertaken as a consequence of an intercomparison made under project no 341 which identified serious difficulties in the analysis of folate in food.

The objective is to examine through intercomparisons the performance of the different types of method with a view to identifying improved and more specific procedures for folate possibly based on HPLC or immunoassays which can offer potentially greater sensitivity. The folate content of several food RMs will be certified if it is feasible.

Programme(s)

• FP2-BCR 4 - Research and development programme (EEC) in the field of applied metrology and chemical analysis (Community Bureau of Reference - BCR), 1988-1992

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Laboratory of the Government Chemist (LGC)

Address

Queen's Road
TW11 0LY Teddington
United Kingdom

Participants (14)

Aston University

United Kingdom

Address

Aston Triangle
B4 7ET Birmingham

CSIC - Instituto del Frio

Spain

Address

Ciudad Universitaria
28040 Madrid

Federal Research Centre for Nutrition

Germany

Address

Haid-und-neustr.9
76131 Karlsruhe

Justus-Liebig-Universität Giessen

Germany

Address

Wilhelmstraße 20
35398 Giessen

National Food Administration

Sweden

Address

5,Hamnesplanaden
75126 Uppsala

National Food Agency of Denmark

Denmark

Address

19,Mörkhöj Bygade
2860 Söborg

Steins Laboratorium

Denmark

Address

8,Hjaltesvej
7500 Holstebro

TNO Toxicology and Nutrition Institute

Netherlands

Address

3700 AJ Zeist

UNIVERSITAET - GESAMTHOCHSCHULE PADERBORN

Germany

Address

Warburger Strasse 100
33098 Paderborn

Unilever UK Central Resources Ltd

United Kingdom

Address

Colworth House
MK44 1LQ Sharnbrook

University College of Cork

Ireland

Address

Cork

University of Lund and Institute of Technology

Sweden

Address

22100 Lund

University of Lund and Institute of Technology

Sweden

Address

22100 Lund

Universität Basel

Switzerland

Address

Vesalianum, Vesalgasse 1
4051 Basel