Objective
One of the key enzymes involved in moth phermone biosynthesis, palmitoyl coenzyme A Delta-11 desaturase, has been characterized in subcellular fractions from pheromonal glands of adult female Spodoptera littoralis. Desaturase activity was dependent on the presence of pyridine nucleotides with nicotinamide adenine dinucleotide phosphate (NADP) better electron donor than reduced NADP (NADPH). Incubation with free acids showed the highest activity for palmitic acid followed by myristic and stearic acids. (Z)-11-hexadecenoic acid (methyl ester) was the product of desaturase activity. The optimal pH for the enzyme was 6.8-7.2 at 25 C.
With the general aim to make insect semiochemicals more widely available for crop protection, the production of different insect sex pheromones by genetically engineered crop plants will be studied. Biosynthetic routes to Lepidopteran sex pheromones involved as crucial step dehydrogenation at specific sites of C18 and C16 aliphatic chains. In the present study the gene responsible for producing the -11 desaturase would be transferred to a crop plant to enable it to provide the source of Lepidopteran sex phermones with C16 carbon chains unsaturated at C11, such as those of several important pests, diamondback moth Pluttellaxylostel and Sesamia spp. enabling insect mating disruption.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- agricultural sciences agriculture, forestry, and fisheries agriculture agronomy plant protection
- natural sciences biological sciences genetics nucleotides
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
MADRID
Spain
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