The project builds on complementary skills in Belgian and British laboratories. Ultrastructural studies are proposed on adherence of clinical isolates of Campylobacter to human intestinal enterocytes and on subsequent invasion and cell damage, and assays for stages in infection will be developed in parallel studies with cultures cells. Preliminary work on iron deprivation of Campylobacter will be extended to include immunological studies of iron regulated outer membrane proteins, and their possible role as siderophore receptors. Gene libraries of Campylobacter DNA will be made in E-coli, and screened for expression of virulence determinants and erythromycin resistance, either directly or by the use of shuttle vectors.
Funding SchemeCSC - Cost-sharing contracts