- To determine whether the fungal species Ascochyta fabae and A. rabiei produce phytotoxic compounds that play a role in virulence of the fungi for their respective hosts;
- To formulate potential procedures for using such phytotoxic compounds in the selection and breeding of resistant genotypes.
- Both fungi were found to produce toxins in culture. A. fabae produced a compound that was isolated and identified as ascochitine. A. rabiei produced three compounds that were isolated and identified as solanapyrones A, B and C.
- A defined medium was formulated for the production of the solanapyrone toxins by A. rabiei.
- A high performance liquid chromatography system was formulated for the separation and quantitative estimation of the solanapyrone toxins from complex mixtures such as culture filtrates and plant products.
- Ascochitine production by A. fabae was not related to virulence of isolates of the fungus.
- Solanapyrone production by A. rabiei was related to virulence of isolates of the fungus.
- Cultivars of chickpea that were less sensitive to the solanapyrone toxins were also more resistant to A. rabiei.
- To aid the breeding of elite lines of faba bean and chickpea with resistance to their respective Ascochyta pathogens;
- To strengthen research training links among North African countries and countries of the Indian Subcontinent with European centres, in particular the exchange of scientists from Tunisia and Pakistan, funded by the British Council and the World Bank. The Pakistan postgraduate is following up the pea of the project related to blight of chickpea caused by Ascochyta rabiei.
Funding SchemeCSC - Cost-sharing contracts