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HEPATIC MICROFLUIDIC BIOREACTOR

Objective

The goal of HeMiBio is to develop a hepatic microfluidic bioreactor from human iPSC-derived hepatocytes, hepatic sinusoidal endothelial cells (HSEC) and stellate cells (HSC), suitable for inclusion in a repeated dose toxicity testing strategy of pharmaceuticals/cosmetic ingredients. The successful creation of such a liver-device requires (a) homotypic and heterotypic interactions between the three cell types to induce and maintain their functional, differentiated state, and (b) optimisation of the matrix, oxygenation conditions, nutrient transport and physiological shear forces. The objectives are (1) to engineer the cellular components incorporated in the bioreactor to enable specific and spatially defined enrichment of the different cells from iPSC progeny, and, by gene editing, to allow non-invasive monitoring of the cellular state (differentiation and damage). (2) Aside from the molecular sensors, an array of electro-chemical sensors will be embedded in the reactors to assess liver-specific function and cellular health under repeated dose toxicity conditions, dynamically and in a high-throughput way. Cells and sensors will be built into (3) bioreactors that will be sequentially upgraded from 2D to 3D microfluidic reactors to ultimately allow full maintenance of mature functional hepatocytes, HSC and HSEC for >28 days. (4) As the ultimate goal is to use the device as a human-based alternative to rodent long-term hepatotoxicity studies, it will be of utmost importance to provide proof of concept that the 3D-devices reveal the hepatotoxicity of prototypical hepatotoxic compounds in vivo (5). “-Omics” and cell functionality studies will provide evidence that liver-like cells are present, exposed and affected by the selected toxic compounds. These ambitious objectives will be achieved by the excellent project team, composed of academic/industrial partners with unique and complementary biology, physiology, toxicology and technical skills from 7 EU Member States.

Call for proposal

FP7-HEALTH-2010-Alternative-Testing
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Coordinator

KATHOLIEKE UNIVERSITEIT LEUVEN
Address
Oude Markt 13
3000 Leuven
Belgium
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 788 019,20
Administrative Contact
Elke Lammertyn (Dr.)

Participants (12)

VRIJE UNIVERSITEIT BRUSSEL
Belgium
EU contribution
€ 585 899,65
Address
Pleinlaan 2
1050 Brussel
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Maria Vereeken (Ms.)
UNIVERSITETET I OSLO
Norway
EU contribution
€ 279 561,31
Address
Problemveien 5-7
0313 Oslo
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Eva Helene Mjelde (Ms.)
CONSORCI INSTITUT D'INVESTIGACIONS BIOMEDIQUES AUGUST PI I SUNYER
Spain
EU contribution
€ 276 801,80
Address
Calle Rossello 149 Puerta Bjs
08036 Barcelona
Activity type
Other
Administrative Contact
Pastora Martinez Samper (Ms.)
THE HEBREW UNIVERSITY OF JERUSALEM
Israel
EU contribution
€ 480 144,40
Address
Edmond J Safra Campus Givat Ram
91904 Jerusalem
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jane Turner (Ms.)
INTERUNIVERSITAIR MICRO-ELECTRONICA CENTRUM
Belgium
EU contribution
€ 283 237,65
Address
Kapeldreef 75
3001 Leuven
Activity type
Research Organisations
Administrative Contact
Christine Van Houtven (Ms.)
FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
Germany
EU contribution
€ 471 533,61
Address
Hansastrasse 27C
80686 Munchen
Activity type
Research Organisations
Administrative Contact
Andrea Zeumann (Ms.)
CSEM CENTRE SUISSE D'ELECTRONIQUE ET DE MICROTECHNIQUE SA - RECHERCHE ET DEVELOPPEMENT
Switzerland
EU contribution
€ 282 953,63
Address
Rue Jaquet Droz 1
2000 Neuchatel
Activity type
Research Organisations
Administrative Contact
Olivier Guenat (Dr.)
MEDICYTE GMBH

Participation ended

Germany
EU contribution
€ 279 578,83
Address
Im Neuenheimer Feld 581
69120 Heidelberg
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Stefan Holder (Mr.)
MEDIZINISCHE HOCHSCHULE HANNOVER

Participation ended

Germany
EU contribution
€ 87 768,62
Address
Carl-neuberg-strasse 1
30625 Hannover
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Frank Dittrich (Mr.)
UNIVERSITETET I TROMSOE - NORGES ARKTISKE UNIVERSITET
Norway
EU contribution
€ 281 749,57
Address
Hansine Hansens Veg 14
9019 Tromso
Activity type
Higher or Secondary Education Establishments
Administrative Contact
ørjan Olsen (Mr.)
INSERM TRANSFERT SA
France
EU contribution
€ 413 878,35
Address
Rue Watt 7
75013 Paris
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Louis Jammayrac (Mr.)
UNIVERSITAETSKLINIKUM FREIBURG
Germany
EU contribution
€ 188 873,38
Address
Hugstetter Strasse 49
79106 Freiburg
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Juergen Dreyer (Mr.)