Objectif In several gastrointestinal diseases such as irritable bowel syndrome or inflammatory bowel diseases, proteolytic homeostasis is disrupted. This disruption has an important impact of the pathophysiology of those diseases. We hypothesized that intestinal epithelial cells are major regulators of this homeostasis by secreting proteases and protease inhibitors, thereby interfering with gut biological functions and enteric neuron biology.We have generated preliminary data demonstrating that stimulated intestinal epithelial cells release proteolytic activity predominantly on the basolateral side, suggesting that epithelium-derived proteases could then modify mucosal physiology and participate to the dialogue between epithelium and other mucosal cells such as enteric neurons.The aim of the project is to:1/ characterize the protease/anti-protease balance issued from intestinal epithelium,2/ study the impact of intestinal epithelium-derived proteolytic machinery on epithelial physiological functions3/ study the impact of intestinal epithelium-derived proteolytic factors on enteric nervous system activationWe believe that results raised from this study will identify previously unknown actors of intestine physiology and pathophysiology and of the neuro-epithelial crosstalks in the gut. Further, we believe that results generated from the present study could have implications more largely on our understanding of mucosal physiology. Champ scientifique natural sciencesbiological sciencesneurobiologymedical and health sciencesbasic medicinephysiologypathophysiologymedical and health sciencesclinical medicinegastroenterologyinflammatory bowel diseasemedical and health sciencesbasic medicinephysiologyhomeostasis Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) ERC-SG-LS4 - ERC Starting Grant - Physiology, Pathophysiology and Endocrinology Appel à propositions ERC-2012-StG_20111109 Voir d’autres projets de cet appel Régime de financement ERC-SG - ERC Starting Grant Institution d’accueil INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Contribution de l’UE € 1 287 000,00 Adresse RUE DE TOLBIAC 101 75654 Paris France Voir sur la carte Région Ile-de-France Ile-de-France Paris Type d’activité Research Organisations Chercheur principal Nathalie Vergnolle (Dr.) Contact administratif Muriel Bouas (Mrs.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Bénéficiaires (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE France Contribution de l’UE € 1 287 000,00 Adresse RUE DE TOLBIAC 101 75654 Paris Voir sur la carte Région Ile-de-France Ile-de-France Paris Type d’activité Research Organisations Chercheur principal Nathalie Vergnolle (Dr.) Contact administratif Muriel Bouas (Mrs.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée