Objetivo Glycosyl transferases (GT) are extremely efficient biocatalysts that can be used for the synthesis of special carbohydrates and glycoconjugates. Famous examples of such products include human milk oligosaccharides that serve as prebiotics, and glycosides of flavonoids with improved stability and solubility. Unfortunately, the large-scale application of GT has been hampered by their low operational stability and by the high cost of their glycosyl donor. This project aims to solve both problems by a combination of enzyme and process engineering. On the one hand, the production of stable biocatalysts will be facilitated by the development of a suitable expression system, the design of optimized variants and the development of immobilized formulations. On the other hand, the production and recycling of nucleotide-activated sugars will be accomplished by exploiting the reaction of sucrose synthase (SuSy).With the help of SuSy, UDP-glucose can be produced from sucrose as cheap and abundant substrate. Furthermore, other nucleotide sugars can be obtained when sucrose analogues are employed. These alternative substrates will be produced here with fructansucrase (FS), an enzyme that can couple fructose to various monosaccharides. However, the activity of both FS and SuSy towards sucrose analogues will have to be improved to become economically viable. In that way, the proposed concept can be developed into a generic procedure, in which the nucleotide moiety can be recycled to establish a constant supply of glycosyl donor. The three enzymatic steps will be optimized and integrated into an efficient process for the production of glycosylated compounds. The economical potential of this technology will be demonstrated by the scale-up of selected reactions at the industrial facilities available in our consortium. The participants in this project comprise 3 universities, 1 research institute and 4 SME with complementary skills and expertise. Ámbito científico engineering and technologyenvironmental engineeringwaste managementwaste treatment processesrecyclingnatural sciencesbiological sciencesbiochemistrybiomoleculescarbohydratesnatural sciencesbiological sciencesgeneticsnucleotidesnatural scienceschemical sciencescatalysisbiocatalysisnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programa(s) FP7-KBBE - Specific Programme "Cooperation": Food, Agriculture and Biotechnology Tema(s) KBBE.2013.3.3-04 - Optimal and cost-effective industrial biocatalysts Convocatoria de propuestas FP7-KBBE-2013-7-single-stage Consulte otros proyectos de esta convocatoria Régimen de financiación CP-TP - Collaborative Project targeted to a special group (such as SMEs) Coordinador UNIVERSITEIT GENT Aportación de la UE € 1 229 682,00 Dirección SINT PIETERSNIEUWSTRAAT 25 9000 Gent Bélgica Ver en el mapa Región Vlaams Gewest Prov. Oost-Vlaanderen Arr. Gent Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Evelien Vandevelde (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Participantes (7) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo TECHNISCHE UNIVERSITAET GRAZ Austria Aportación de la UE € 837 300,00 Dirección RECHBAUERSTRASSE 12 8010 Graz Ver en el mapa Región Südösterreich Steiermark Graz Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Bernd Nidetzky (Prof.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG Alemania Aportación de la UE € 735 708,00 Dirección SANDERRING 2 97070 Wuerzburg Ver en el mapa Región Bayern Unterfranken Würzburg, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Christian Gloggengiesser (Mr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS España Aportación de la UE € 333 650,00 Dirección CALLE SERRANO 117 28006 Madrid Ver en el mapa Región Comunidad de Madrid Comunidad de Madrid Madrid Tipo de actividad Research Organisations Contacto administrativo Maria Angeles López Vázquez (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos BIO-PRODICT BV Países Bajos Aportación de la UE € 470 364,00 Dirección NIEUWE MARKTSTRAAT 54 6511 AA Nijmegen Ver en el mapa Región Oost-Nederland Gelderland Arnhem/Nijmegen Tipo de actividad Private for-profit entities (excluding Higher or Secondary Education Establishments) Contacto administrativo Henk-Jan Joosten (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos ACIB GMBH Austria Aportación de la UE € 582 163,00 Dirección KRENNGASSE 37/2 8010 Graz Ver en el mapa Región Südösterreich Steiermark Graz Tipo de actividad Research Organisations Contacto administrativo Julia Studencki (Mrs.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos C-LECTA GMBH Alemania Aportación de la UE € 451 100,00 Dirección PERLICKSTRASSE 5 04103 Leipzig Ver en el mapa Región Sachsen Leipzig Leipzig Tipo de actividad Private for-profit entities (excluding Higher or Secondary Education Establishments) Contacto administrativo Carsten Fietz (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos GALAB LABORATORIES GMBH Alemania Aportación de la UE € 449 675,00 Dirección AM SCHLEUSENGRABEN 7 21029 Hamburg Ver en el mapa Región Hamburg Hamburg Hamburg Tipo de actividad Private for-profit entities (excluding Higher or Secondary Education Establishments) Contacto administrativo Jürgen Kuballa (Dr.) Enlaces Contactar con la organización Opens in new window Coste total Sin datos