Objetivo Light-sensitive channel proteins have attracted much attention as functional molecules, as they possess unique conformations and functions depending on their respective irradiation states. Embedding these proteins in heterogeneous cellular frameworks enabled to gain light-control of cellular and in particular neuronal behaviour. However to date, optogenetic solutions that address endogenous, intracellular biomolecules in a universal fashion remain elusive. The project will apply design and selection strategies aiming at nucleic acid molecules that can be controlled by irradiation with light and which we have developed in preliminary studies. This will now be taken significant steps forward by generating modular allosteric ribonucleic acid (RNA) assemblies that respond to light. These assemblies provide a generic solution and represent long-sought methods to complement the optogenetic toolbox. The aim of this project is the generation of allosteric molecules built from at least two RNA domains; one domain that binds to a soluble photoreceptor protein (PRP) in a light-dependent manner and a second RNA domain, whose protein inhibiting function in turn depends on the binding state of the PRP-recognizing part. We will construct light-responsive allosteric RNA assemblies that can be ubiquitously used in cells and in vivo, independent of specific model organisms, for optogenetic control and spatiotemporal analysis of endogenous, intracellular biomolecule function. The project is highly interdisciplinary and will open novel routes for biomolecule analysis in cells and in vivo. It has implications ranging from life sciences to optogenetics, and from combinatorial biochemistry to synthetic biology. As these new tools will be applicable by any scientist to analyse protein function at high spatiotemporal resolution, the project bears an enormous innovative potential. Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencessynthetic biologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsRNA Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-CG-2013-LS9 - ERC Consolidator Grant - Applied Life Sciences and Non-Medical Biotechnology Convocatoria de propuestas ERC-2013-CoG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-CG - ERC Consolidator Grants Institución de acogida RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITAT BONN Aportación de la UE € 1 992 438,00 Dirección REGINA PACIS WEG 3 53113 Bonn Alemania Ver en el mapa Región Nordrhein-Westfalen Köln Bonn, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Investigador principal Günter Mayer (Prof.) Contacto administrativo Daniela Hasenpusch (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITAT BONN Alemania Aportación de la UE € 1 992 438,00 Dirección REGINA PACIS WEG 3 53113 Bonn Ver en el mapa Región Nordrhein-Westfalen Köln Bonn, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Investigador principal Günter Mayer (Prof.) Contacto administrativo Daniela Hasenpusch (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos