Objetivo Development relies on a complex network of molecular effectors that ultimately modify the mechanical properties of cells and control shape changes. In turn, mechanical forces can also feedback on the molecular network to govern development. Several mechanosensitive proteins have been identified in animals but their role in multicellular development remains poorly documented. Plants are ideal systems to study mechanotransduction in development because their mechanics is mainly mediated by the cell wall. Here we propose to identify the plant mechanotransduction pathways and investigate their role in development, using Arabidopsis cell culture, cotyledons and shoot apical meristems as experimental systems.We will conduct a multiscale investigation into mechanotransduction, at the level of a cell, of a small group of cells, and of the tissue. We have already characterized the response of microtubules to mechanical stress using a set of micromechanical tools in these three systems, and we have identified a mechanosensing protein, which, when impaired, causes growth coordination defects in the epidermis and altered morphogenesis. We will identify and characterize other elements of the plant mechanotransduction pathways, including mechanosensitive genes and proteins, based on preliminary results and on a well-designed screen. Scaling up, we will generate transgenic lines in which patches of cells with altered mechanical properties can be induced, and we will test whether mechanical heterogeneities are used to coordinate cell wall remodeling and cell division in neighboring cells. Last, we will investigate whether these biophysical coordinating mechanisms contribute to patterning and cell fate in organs, focusing on the epidermis, the boundaries between expression domains, and stem cell identity. We will investigate whether mutants exhibiting identity defects can be rescued with applied mechanical forces or locally modified mechanical properties. Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesmedical biotechnologycells technologiesstem cells Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-CG-2013-LS3 - ERC Consolidator Grant - Cellular and Developmental Biology Convocatoria de propuestas ERC-2013-CoG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-CG - ERC Consolidator Grants Institución de acogida INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT Aportación de la UE € 1 844 991,99 Dirección 147 RUE DE L'UNIVERSITE 75007 Paris Francia Ver en el mapa Región Ile-de-France Ile-de-France Paris Tipo de actividad Research Organisations Contacto administrativo Remy Beaufrere (Mr.) Investigador principal Olivier Thomas Hamant (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT Francia Aportación de la UE € 1 844 991,99 Dirección 147 RUE DE L'UNIVERSITE 75007 Paris Ver en el mapa Región Ile-de-France Ile-de-France Paris Tipo de actividad Research Organisations Contacto administrativo Remy Beaufrere (Mr.) Investigador principal Olivier Thomas Hamant (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Reino Unido Aportación de la UE € 24 831,01 Dirección TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge Ver en el mapa Región East of England East Anglia Cambridgeshire CC Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Renata Schaeffer (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos
