Objective Overcoming the barriers that the cell envelope and efflux pumps provide to Gram-negative bacteria is a major bottleneck in the discovery and development of new antibiotics. To respond to this Call we are extending our current network on antibiotic translocation and propose an ambitious project ranging from identification of novel resistance mechanisms in clinical bacterial isolates to crystallization of molecular resistance components and all-atom modeling to determine the kinetics of permeation. Our particular strength lies in the identification and quantification of transport proteins involved in membrane permeation at the cellular level combined with our expertise in quantifying the rate limiting steps of translocation at atomic level. Based on our experience we intend to:1. develop multiple assays to determine intracellular concentrations of small molecules2. increase the overall structural knowledge of outer membrane proteins3. extend knowledge on active-uptake transport systems, such as iron transport4. identify novel targets whose modulation can alter the penetration of other small molecules5. obtain as complete a picture as possible of small molecule penetration into and efflux out of bacterial cellsCombining the research question at different levels we expect to understand the complexity of membrane permeability. In particular, we are convinced that our molecular approach is able to provide a general template for a guided design of antibiotics to achieve high permeability. In addition, we will create a data base to allow the sharing of antibacterial R&D information on an unprecedented level. Also, via careful analysis of this information we intend to provide learnings as best practices that will lead to an increase in the overall efficiency of antibacterial R&D. Fields of science natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantibiotics Programme(s) FP7-JTI - Specific Programme "Cooperation": Joint Technology Initiatives Topic(s) IMI-JU-06-2012-02 - ND4BB Topic 2: Learning From Success And Failure & Getting Drugs Into Bad Bugs Call for proposal IMI-JU-06-2012 See other projects for this call Funding Scheme JTI-CP-IMI - Joint Technology Initiatives - Collaborative Project (IMI) Coordinator GLAXOSMITHKLINE RESEARCH AND DEVELOPMENT LTD. EU contribution No data Address Great West Road 980 TW89GS Brentford United Kingdom See on map Region London Outer London — West and North West Hounslow and Richmond upon Thames Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Principal investigator Robert Stavenger (Dr) Administrative Contact Miles Sally (-) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Participants (27) Sort alphabetically Sort by EU Contribution Expand all Collapse all CONSTRUCTOR UNIVERSITY BREMEN GGMBH Germany EU contribution € 4 178 960,00 Address CAMPUS RING 1 28725 Bremen See on map Region Bremen Bremen Bremen, Kreisfreie Stadt Activity type Higher or Secondary Education Establishments Administrative Contact Ronald Kieschnick (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITA DEGLI STUDI DI CAGLIARI Italy EU contribution € 1 678 960,00 Address VIA UNIVERSITA 40 09124 Cagliari See on map Region Isole Sardegna Cagliari Activity type Higher or Secondary Education Establishments Administrative Contact Luciano Colombo (Prof.) Links Contact the organisation Opens in new window Total cost No data THE UNIVERSITY COURT OF THE UNIVERSITY OF ST ANDREWS United Kingdom EU contribution € 886 152,00 Address NORTH STREET 66 COLLEGE GATE KY16 9AJ St Andrews See on map Region Scotland Eastern Scotland Clackmannanshire and Fife Activity type Higher or Secondary Education Establishments Administrative Contact Trish Starrs (Ms) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN Germany EU contribution € 499 000,00 Address THEODOR W ADORNO PLATZ 1 60323 Frankfurt Am Main See on map Region Hessen Darmstadt Frankfurt am Main, Kreisfreie Stadt Activity type Higher or Secondary Education Establishments Administrative Contact Kristina Wege (Ms) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN Ireland EU contribution € 90 313,00 Address BELFIELD 4 Dublin See on map Region Ireland Eastern and Midland Dublin Activity type Higher or Secondary Education Establishments Administrative Contact Donal Doolan (Mr) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data NANION TECHNOLOGIES GMBH Germany EU contribution € 293 200,00 Address GANGHOFERSTRASSE 70A 80339 Munchen See on map Region Bayern Oberbayern München, Kreisfreie Stadt Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Niels Fertig (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data BRUKER DALTONIK GMBH Germany EU contribution No data Address FAHRENHEITSTRASSE 4 28359 BREMEN See on map Region Bremen Bremen Bremen, Kreisfreie Stadt Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Tanja Hohnhorst (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data FUNDACION PRIVADA INSTITUTO DE SALUD GLOBAL BARCELONA Spain EU contribution € 203 639,00 Address C ROSSELLO 08036 Barcelona See on map Administrative Contact Jordi Vila (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data IONOVATION GMBH Germany EU contribution € 450 400,00 Address WESTERBREITE 7 49084 OSNABRUCK See on map Region Niedersachsen Weser-Ems Osnabrück, Kreisfreie Stadt Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Karsten Gall (Dr.) Links Contact the organisation Opens in new window Total cost No data UNIVERSITY OF NEWCASTLE UPON TYNE United Kingdom EU contribution € 894 625,00 Address KINGS GATE NE1 7RU Newcastle Upon Tyne See on map Region North East (England) Northumberland and Tyne and Wear Tyneside Activity type Higher or Secondary Education Establishments Administrative Contact Amanda Gregory (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITAETSKLINIKUM FREIBURG Germany EU contribution € 299 351,00 Address HUGSTETTER STRASSE 49 79106 Freiburg See on map Region Baden-Württemberg Freiburg Freiburg im Breisgau, Stadtkreis Activity type Higher or Secondary Education Establishments Administrative Contact Katharina Strub (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITE DE GENEVE Switzerland EU contribution € 937 537,00 Address RUE DU GENERAL DUFOUR 24 1211 Geneve See on map Region Schweiz/Suisse/Svizzera Région lémanique Genève Activity type Higher or Secondary Education Establishments Administrative Contact Christian Van Delden (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITAT BASEL Switzerland EU contribution € 1 221 131,00 Address PETERSPLATZ 1 4051 Basel See on map Region Schweiz/Suisse/Svizzera Nordwestschweiz Basel-Stadt Activity type Higher or Secondary Education Establishments Administrative Contact Rachel Benzies (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data European ScreeningPort GmbH Participation ended Germany EU contribution € 232 618,00 Address Schnackenburgallee 22525 Hamburg See on map Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Mira Grättinger (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Nanospot GmbH Participation ended Germany EU contribution € 57 284,00 Address Johann-Krane-Weg 48149 Münster See on map Administrative Contact Sebastian Giehring (Dr) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data SYNCHROTRON SOLEIL SOCIETE CIVILE France EU contribution € 304 000,00 Address L ORME DES MERISIERS 91190 Saint Aubin See on map Region Ile-de-France Ile-de-France Essonne Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Yves Le Ray (Mr) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data ASSISTANCE PUBLIQUE HOPITAUX DE PARIS France EU contribution € 103 931,00 Address 55 BOULEVARD DIDEROT 75012 Paris See on map Region Ile-de-France Ile-de-France Val-de-Marne Activity type Research Organisations Administrative Contact Alix Pillot (Ms) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Yelen France EU contribution € 110 263,00 Address Boulevard Tempête 13820 Ensues La Redonne See on map Administrative Contact Nicolas VIDAL (Engineer) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITE D'AIX MARSEILLE France EU contribution € 1 653 621,00 Address BOULEVARD CHARLES LIVON 58 LE PHARO 13284 Marseille See on map Region Provence-Alpes-Côte d’Azur Provence-Alpes-Côte d’Azur Bouches-du-Rhône Activity type Higher or Secondary Education Establishments Administrative Contact Celine Damon (Ms) Links Contact the organisation Opens in new window Total cost No data SANOFI-AVENTIS RECHERCHE & DEVELOPPEMENT France EU contribution No data Address 82 AVENUE RASPAIL 94250 GENTILLY See on map Region Ile-de-France Ile-de-France Val-de-Marne Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Nathalia Murillo (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data ASTRAZENECA AB Sweden EU contribution No data Address Vastra Maelarhamnen 151 85 Sodertaelje See on map Region Östra Sverige Stockholm Stockholms län Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Eva Lindgren (Senior Director) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data BASILEA PHARMACEUTICA INTERNATIONAL AG Switzerland EU contribution No data Address GRENZACHERSTRASSE 487 4058 BASEL See on map Region Schweiz/Suisse/Svizzera Nordwestschweiz Basel-Stadt Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Laurenz Kellenberger (Dr) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data JANSSEN PHARMACEUTICA NV Belgium EU contribution No data Address TURNHOUTSEWEG 30 2340 Beerse See on map Region Vlaams Gewest Prov. Antwerpen Arr. Turnhout Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Kristien Bonroy (Ms) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS France EU contribution € 540 600,00 Address RUE MICHEL ANGE 3 75794 Paris See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Administrative Contact Gaëlle BUJAN (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data THE HYVE BV Netherlands EU contribution € 326 064,00 Address ARTHUR VAN SCHENDELSTRAAT 650 3511 MJ Utrecht See on map Region West-Nederland Utrecht Utrecht Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Kees Van Bochove (Msc.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data GRITSYSTEMS AS Denmark EU contribution € 349 875,00 Address TREKRONERGADE 126F, 3 TV 2500 Valby See on map Region Danmark Hovedstaden Byen København Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Claus Stie Kallesøe (Mr) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data FRAUNHOFER GESELLSCHAFT ZUR FORDERUNG DER ANGEWANDTEN FORSCHUNG EV Germany EU contribution € 672 679,00 Address HANSASTRASSE 27C 80686 Munchen See on map Region Bayern Oberbayern München, Kreisfreie Stadt Activity type Research Organisations Administrative Contact Michael Prestele (Mr) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data