Objetivo Geobacillus spp are thermophilic Gram positive bacteria which are specialists in the degradation of hemicellulose derived from renewable biological feedstocks and the supervisor’s group has recently engineered strains able to degrade cellulose. Some of the strains are facultative anaerobes which can grow anaerobically and produce fermentation products such as lactate and ethanol. Hence they are an ideal platform for producing fermentation products from renewable carbohydrate resources, such as lignocellulose. Recent genome sequencing has shown that some strains have the ability to produce proteinaceous micro-compartments, known from other organisms to host reactions which produce unstable/highly reactive intermediates, and these have been observed by EM analysis in the lab of the supervisor. In this project the MC fellow will exploit some novel technology under development in the lab of the supervisor to develop thermostable alpha-keto acid decarboxylases, which are not typically present in thermophiles. These produce reactive aldehydes which cause toxicity to producing cells, even in the presence of a cognate alcohol dehydrogenase, but this can potentially be reduced by targeting the decarboxylase and alcohol dehydrogenase to a protein micro-compartment. Therefore, the aim of this project is to establish the rules for targeting enzymes to protein micro-compartments in Geobacillus spp and exploit these using thermophilic decarboxylase – dehydrogenase combinations to produce 1) ethanol, 2) isobutanol. The final constructs will be evaluated against real lignocellulosic feedstocks in the lab of the industrial collaborator ReBio Ltd. The project will provide an in depth training in metabolic and cellular engineering, employing synthetic biology strategies. Ámbito científico natural scienceschemical sciencesorganic chemistryaldehydesnatural sciencesbiological sciencessynthetic biologynatural scienceschemical sciencesorganic chemistryalcoholsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymesengineering and technologyindustrial biotechnologybioprocessing technologiesfermentation Programa(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Tema(s) MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Convocatoria de propuestas H2020-MSCA-IF-2015 Consulte otros proyectos de esta convocatoria Régimen de financiación MSCA-IF-EF-CAR - CAR – Career Restart panel Coordinador UNIVERSITY OF BATH Aportación neta de la UEn € 195 454,80 Dirección CLAVERTON DOWN BA2 7AY Bath Reino Unido Ver en el mapa Región South West (England) Gloucestershire, Wiltshire and Bristol/Bath area Bath and North East Somerset, North Somerset and South Gloucestershire Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 195 454,80
