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4D analysis of heart development and regeneration using advanced light microscopy

4D analysis of heart development and regeneration using advanced light microscopy

Objective

Cardiovascular (CV) disease is a main cause of death worldwide. During adulthood, ischemic heart disease leads to heart failure and perinatally, congenital heart defects are found in over 20% of deaths. Moreover, genetic or epigenetic factors altering development can have an impact much later in life. These facts underscore the need of a better understanding of the genetic and environmental factors that influence CV development. An important way to increase our knowledge is by visualizing cardiac development in vivo. Recent advance in microscopy allows monitoring CV development at a cellular level in organisms such as the zebrafish model. Particularly revolutionary has been the development of light sheet microscopy (LSM). We want to further exploit LSM for in vivo manipulation of cells in the embryonic zebrafish heart and measure with high precision biophysical parameters, by introducing novel features to LSM such as optical tweezers. High throughput cardiac imaging protocols for zebrafish larvae suitable for screenings will be set up. We will develop softwares to enhance resolution of acquisition, large dataset handling and image-processing. The aim is to generate a toolbox to be implemented into existing software packages allowing a complete modeling of zebrafish cardiac morphogenesis. We will adapt LSM for adult zebrafish hearts to study cardiac regeneration and mouse heart development at cellular resolution. Each Early Stage Researchers (ESRs) will develop their own technology to solve a biological problem at the frontier of knowledge. ESRs will receive multidisciplinary (CV development, physics, biocomputing, bioimaging) as well as intersectorial (academic research, SMEs, large companies) training and will achieve unique skills on microscopy, in-vivo imaging and image analysis allowing them to interrogate questions on cardiac development and regeneration. Their profile will be at the interface of a bioengineer and a life science researcher filling a currently existing gap on the market.

Coordinator

CENTRO NACIONAL DE INVESTIGACIONESCARDIOVASCULARES CARLOS III (F.S.P.)

Address

Calle Melchor Fernandez Almagro 3
28029 Madrid

Spain

Activity type

Research Organisations

EU Contribution

€ 247 872,96

Participants (7)

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ACQUIFER AG

Germany

Leica Microsystems CMS GmbH

Germany

EU Contribution

€ 249 216,48

CENTRE EUROPEEN DE RECHERCHE EN BIOLOGIE ET MEDECINE

France

EU Contribution

€ 262 875,60

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

France

EU Contribution

€ 131 437,80

UNIVERSITAET BERN

Switzerland

EU Contribution

€ 265 226,76

PHILIPS IBERICA SA

Spain

EU Contribution

€ 247 872,96

DITABIS DIGITAL BIOMEDICAL IMAGING SYSTEMS AG

Germany

EU Contribution

€ 124 608,24

Partners (7)

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UNIVERSIDAD AUTONOMA DE MADRID

UNIVERSIDAD CARLOS III DE MADRID

UNIVERSITE DE STRASBOURG

INSTITUTO DE EMPRESA SL

FONDATION DE L'INSTITUT DE RECHERCHE IDIAP

Juan Sarasua

ECOLE POLYTECHNIQUE

Project information

Grant agreement ID: 722427

Status

Ongoing project

  • Start date

    1 January 2017

  • End date

    31 December 2020

Funded under:

H2020-EU.1.3.1.

  • Overall budget:

    € 1 529 110,80

  • EU contribution

    € 1 529 110,80

Coordinated by:

CENTRO NACIONAL DE INVESTIGACIONESCARDIOVASCULARES CARLOS III (F.S.P.)

Spain