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4D analysis of heart development and regeneration using advanced light microscopy

Objective

Cardiovascular (CV) disease is a main cause of death worldwide. During adulthood, ischemic heart disease leads to heart failure and perinatally, congenital heart defects are found in over 20% of deaths. Moreover, genetic or epigenetic factors altering development can have an impact much later in life. These facts underscore the need of a better understanding of the genetic and environmental factors that influence CV development. An important way to increase our knowledge is by visualizing cardiac development in vivo. Recent advance in microscopy allows monitoring CV development at a cellular level in organisms such as the zebrafish model. Particularly revolutionary has been the development of light sheet microscopy (LSM). We want to further exploit LSM for in vivo manipulation of cells in the embryonic zebrafish heart and measure with high precision biophysical parameters, by introducing novel features to LSM such as optical tweezers. High throughput cardiac imaging protocols for zebrafish larvae suitable for screenings will be set up. We will develop softwares to enhance resolution of acquisition, large dataset handling and image-processing. The aim is to generate a toolbox to be implemented into existing software packages allowing a complete modeling of zebrafish cardiac morphogenesis. We will adapt LSM for adult zebrafish hearts to study cardiac regeneration and mouse heart development at cellular resolution. Each Early Stage Researchers (ESRs) will develop their own technology to solve a biological problem at the frontier of knowledge. ESRs will receive multidisciplinary (CV development, physics, biocomputing, bioimaging) as well as intersectorial (academic research, SMEs, large companies) training and will achieve unique skills on microscopy, in-vivo imaging and image analysis allowing them to interrogate questions on cardiac development and regeneration. Their profile will be at the interface of a bioengineer and a life science researcher filling a currently existing gap on the market.

Field of science

  • /social sciences/economics and business/business and management/commerce
  • /natural sciences/computer and information sciences/software

Call for proposal

H2020-MSCA-ITN-2016
See other projects for this call

Funding Scheme

MSCA-ITN-EID - European Industrial Doctorates

Coordinator

CENTRO NACIONAL DE INVESTIGACIONES CARDIOVASCULARES CARLOS III (F.S.P.)
Address
Calle Melchor Fernandez Almagro 3
28029 Madrid
Spain
Activity type
Research Organisations
EU contribution
€ 89 509,68

Participants (7)

ACQUIFER AG

Participation ended

Germany
EU contribution
€ 0
Address
Freiburger Strasse 1-3
75179 Pforzheim
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Leica Microsystems CMS GmbH
Germany
EU contribution
€ 249 216,48
Address
Ernst-leitz-strasse 3
35578 Wetzlar
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
CENTRE EUROPEEN DE RECHERCHE EN BIOLOGIE ET MEDECINE
France
EU contribution
€ 262 875,60
Address
Rue Laurent Fries 1
67404 Illkirch Graffenstaden
Activity type
Research Organisations
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France
EU contribution
€ 131 437,80
Address
Rue Michel Ange 3
75794 Paris
Activity type
Research Organisations
UNIVERSITAET BERN
Switzerland
EU contribution
€ 368 370,50
Address
Hochschulstrasse 6
3012 Bern
Activity type
Higher or Secondary Education Establishments
PHILIPS IBERICA SA
Spain
EU contribution
€ 247 872,96
Address
Calle Maria De Portugal 1
28050 Madrid
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
DITABIS DIGITAL BIOMEDICAL IMAGING SYSTEMS AG
Germany
EU contribution
€ 138 453,60
Address
Freiburgerstrasse 3
75179 Pforzheim
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)

Partners (7)

UNIVERSIDAD AUTONOMA DE MADRID
Spain
Address
Calle Einstein 3 Ciudad Univ Cantoblanco Rectorado
28049 Madrid
Activity type
Higher or Secondary Education Establishments
UNIVERSIDAD CARLOS III DE MADRID
Spain
Address
Calle Madrid 126
28903 Getafe (Madrid)
Activity type
Higher or Secondary Education Establishments
UNIVERSITE DE STRASBOURG
France
Address
Rue Blaise Pascal 4
67081 Strasbourg
Activity type
Higher or Secondary Education Establishments
INSTITUTO DE EMPRESA SL
Spain
Address
Calle Maria De Molina 13
28006 Madrid
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
FONDATION DE L'INSTITUT DE RECHERCHE IDIAP
Switzerland
Address
Rue Marconi 19
1920 Martigny
Activity type
Research Organisations
Juan Sarasua
Belgium
Address

Brussels
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
ECOLE POLYTECHNIQUE
France
Address
Route De Saclay
91128 Palaiseau Cedex
Activity type
Higher or Secondary Education Establishments