In Vivo Single-Cell Mechanomics of Bone Adaptation and Regeneration in the Aging Mouse

Project Information

MechAGE

Grant agreement ID: 741883

DOI 10.3030/741883

Project closed

EC signature date 11 July 2017

Start date 1 October 2017

End date 31 March 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 500 000,00

EU contribution € 2 500 000,00

Coordinated by

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland

Publications

Peer reviewed articles (17)

Application of subject-specific adaptive mechanical loading for bone healing in a mouse tail vertebral defect

Author(s): Angad Malhotra; Matthias Walle; Graeme R. Paul; Gisela A. Kuhn; Ralph Müller
Published in: Scientific Reports, Issue 11, 2021, Page(s) 1861, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-81132-8

Trabecular bone remodeling in the aging mouse: A micro-multiphysics agent-based in silico model using single-cell mechanomics

Author(s): Boaretti D, Marques FC, Ledoux C, Singh A, Kendall JJ, Wehrle E, Kuhn GA, Bansod YD, Schulte FA and Müller R
Published in: Frontiers in Bioengineering and Biotechnology, Issue 11, 2023, Page(s) 1091294, ISSN 2296-4185
Publisher: Frontiers Media S.A.
DOI: 10.3389/fbioe.2023.1091294

Real-time finite element analysis allows homogenization of tissue scale strains and reduces variance in a mouse defect healing model

Author(s): Graeme R. Paul; Esther Wehrle; Duncan C. Tourolle; Gisela A. Kuhn; Ralph Müller
Published in: Scientific Reports, Issue 11, 2021, Page(s) 13511, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-92961-y

Hallmarks of frailty and osteosarcopenia in prematurely aged PolgA(D257A/D257A) mice

Author(s): Ariane C. Scheuren,Gommaar D'Hulst,Gisela A. Kuhn,Evi Masschelein,Esther Wehrle,Katrien De Bock,Ralph Müller
Published in: Journal of Cachexia, Sarcopenia and Muscle, 2020, ISSN 2190-6009
Publisher: Blackwell Wiley
DOI: 10.1002/jcsm.12588

Mechano-Regulation of Trabecular Bone Adaptation Is Controlled by the Local in vivo Environment and Logarithmically Dependent on Loading Frequency

Author(s): Scheuren, Ariane C.; Vallaster, Paul; Kuhn, Gisela A.; Paul, Graeme R.; Malhotra, Angad; Kameo, Yoshitaka; Müller, Ralph
Published in: Frontiers in Bioengineering and Biotechnology, Issue 8, 2020, Page(s) 566346, ISSN 2296-4185
Publisher: Frontiers Media SA
DOI: 10.3389/fbioe.2020.566346

Perspectives on in Silico Bone Mechanobiology: Computational Modelling of Multicellular Systems

Author(s): Boaretti, Daniele; id_orcid0000-0001-8411-7465; Wehrle, Esther; id_orcid0000-0003-2723-7342; Bansod, Yogesh Deepak; Tourolle né Betts, Duncan C.; Müller, Ralph; id_orcid0000-0002-5811-7725
Published in: European Cells & Materials, Issue 44, 2021, Page(s) 56-73, ISSN 1473-2262
Publisher: Swiss Society for Biomaterials
DOI: 10.22203/ecm.v044a04

The local and global geometry of trabecular bone.

Author(s): Sebastien J.P. Callens; Duncan C. Tourolle; Ralph Müller; Amir A. Zadpoor
Published in: Acta Biomaterialia, Issue 130, 2021, Page(s) 343-361, ISSN 1742-7061
Publisher: Elsevier BV
DOI: 10.1016/j.actbio.2021.06.013

Tissue-Level Regeneration and Remodeling Dynamics are Driven by Mechanical Stimuli in the Microenvironment in a Post-Bridging Loaded Femur Defect Healing Model in Mice

Author(s): Paul, Graeme R.; Vallaster, Paul; Rüegg, Michelle; Scheuren, Ariane C.; Tourolle, Duncan C.; Kuhn, Gisela A.; Wehrle, Esther; id_orcid0000-0003-2723-7342; Müller, Ralph; id_orcid0000-0002-5811-7725
Published in: Frontiers in Cell and Developmental Biology, Issue 10, 2022, Page(s) 856204, ISSN 2296-634X
Publisher: Frontiers Media S.A.
DOI: 10.3389/fcell.2022.856204

Spatio-temporal characterization of fracture healing patterns and assessment of biomaterials by time-lapsed in vivo micro-computed tomography.

Author(s): Esther Wehrle; Duncan C. Tourolle né Betts; Gisela A. Kuhn; Erica Floreani; Malavika H. Nambiar; Bryant J. Schroeder; Sandra Hofmann; Sandra Hofmann; Ralph Müller
Published in: Scientific Reports, Issue 11, 2021, Page(s) 8660, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-87788-6

The association between mineralised tissue formation and the mechanical local in vivo environment: Time-lapsed quantification of a mouse defect healing model

Author(s): Duncan C. Tourolle né Betts; Esther Wehrle; Graeme R. Paul; Gisela A. Kuhn; Patrik Christen; Sandra Hofmann; Ralph Müller
Published in: Scientific Reports, Issue 10, 2020, Page(s) 1100, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-57461-5

Mechanical Stimuli in the Local In Vivo Environment in Bone: Computational Approaches Linking Organ-Scale Loads to Cellular Signals

Author(s): Graeme R. Paul, Angad Malhotra, Ralph Müller
Published in: Current Osteoporosis Reports, Issue 16/4, 2018, Page(s) 395-403, ISSN 1544-1873
Publisher: Current Science Inc.
DOI: 10.1007/s11914-018-0448-6

Evaluation of longitudinal time-lapsed in vivo micro-CT for monitoring fracture healing in mouse femur defect models

Author(s): Esther Wehrle, Duncan C. Tourolle né Betts, Gisela A. Kuhn, Ariane C. Scheuren, Sandra Hofmann, Ralph Müller
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-53822-x

TRPV4 mediates cell damage induced by hyperphysiological compression and regulates COX2/PGE2 in intervertebral discs.

Author(s): Elena Cambria; Sally Heusser; Ariane C. Scheuren; Wai Kit Tam; Agnieszka Karol; Wolfgang Hitzl; Victor Y. L. Leung; Ralph Müller; Stephen J. Ferguson; Karin Wuertz-Kozak; Karin Wuertz-Kozak
Published in: JOR Spine, Issue 4, 2021, Page(s) e1149, ISSN 2572-1143
Publisher: Wiley
DOI: 10.1002/jsp2.1149

Effects of long-term in vivo micro-CT imaging on hallmarks of osteopenia and frailty in aging mice

Author(s): Ariane C. Scheuren, Gisela A. Kuhn, Ralph Müller
Published in: PLOS ONE, Issue 15/9, 2020, Page(s) e0239534, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0239534

Individualized cyclic mechanical loading improves callus properties during the remodelling phase of fracture healing in mice as assessed from time-lapsed in vivo imaging.

Author(s): Esther Wehrle; Graeme R. Paul; Duncan C. Tourolle né Betts; Gisela A. Kuhn; Ralph Müller
Published in: Scientific Reports, Issue 11, 2021, Page(s) 23037, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-02368-y

Mechanostat parameters estimated from time-lapsed in vivo micro-computed tomography data of mechanically driven bone adaptation are logarithmically dependent on loading frequency

Author(s): Marques FC, Boaretti D, Walle M, Scheuren AC, Schulte FA and Müller R
Published in: Frontiers in Bioengineering and Biotechnology, Issue 11, 2023, Page(s) 1140673, ISSN 2296-4185
Publisher: Frontiers Media S.A.
DOI: 10.3389/fbioe.2023.1140673

Clinical Data for Parametrization of In Silico Bone Models Incorporating Cell-Cytokine Dynamics: A Systematic Review of Literature

Author(s): Ledoux, Charles; Boaretti, Daniele; id_orcid0000-0001-8411-7465; Sachan, Akanksha; Müller, Ralph; id_orcid0000-0002-5811-7725; Collins, Caitlyn J.; id_orcid0000-0003-0181-878X
Published in: Frontiers in Bioengineering and Biotechnology, Issue 10, 2022, Page(s) 901720, ISSN 2296-4185
Publisher: Frontiers Media S.A.
DOI: 10.3389/fbioe.2022.901720

Thesis and dissertations (2)

Longitudinal assessment of frailty and osteosarcopenia in an in vivo model of premature aging

Author(s): Scheuren, Ariane C.
Published in: Doctoral Thesis, Issue 26738, 2020
Publisher: ETH Zurich
DOI: 10.3929/ethz-b-000423860

Individualised multiscale mechanoregulation of fracture healing in mice

Author(s): Paul, Graeme R.
Published in: Doctoral Thesis, Issue 27097, 2020
Publisher: ETH Zurich
DOI: 10.3929/ethz-b-000479421