Objectif Super-resolution methods have recently given new life to fluorescence microscopy; they promise molecular-scale resolution, while maintaining all the benefits of traditional diffraction limited techniques, such as robust labeling methods and three-dimensional imaging capability. However, the current super-resolution techniques only work reliably with thin, brightly labeled, low background samples. STimulated Emission Depletion (STED) super-resolution microscopy in principle is exceptionally well suited for deep imaging, because point-illumination makes it possible to use an optical pinhole that significantly reduces the out-of-focus background signal. However, current STED microscope implementations suffer from very low signal-to-noise ratio (SNR), and the STED depletion beam intensity distribution – that is used to reduce the size of the effective fluorescence volume at the focus – is extremely sensitive to optical aberrations. In AdaptiveSTED project both of these issues will be addressed. The main goal of the AdaptiveSTED project is to develop a real-time aberration correction scheme for STED (and other point-scanning microscopes) that will allow robust, high resolution imaging deep inside complex, aberrating samples. A novel Single Photon Avalanche diode (SPAD) array detector, will make it possible to combine real-time wavefront sensing with high-SNR fluorescence recording into a single detector. The aberration correction scheme will be compatible with any poin-scanning microscopy technique: it will be thoroughly tested with a variety of biological samples in an open-access setting (anyone can use), in STED, two-photon and confocal imaging modes. The aberration correction system will be realized in collaboration with Prof. Martin J. Booth’s group at University of Oxford. Champ scientifique natural sciencesphysical sciencesopticsmicroscopysuper resolution microscopynatural sciencesphysical sciencesopticsmicroscopyconfocal microscopynatural sciencesphysical sciencestheoretical physicsparticle physicsphotons Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Thème(s) MSCA-IF-2017 - Individual Fellowships Appel à propositions H2020-MSCA-IF-2017 Voir d’autres projets de cet appel Régime de financement MSCA-IF-EF-ST - Standard EF Coordinateur FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA Contribution nette de l'UE € 180 277,20 Adresse VIA MOREGO 30 16163 Genova Italie Voir sur la carte Région Nord-Ovest Liguria Genova Type d’activité Research Organisations Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 180 277,20
