Objective
Chromatin functionally organizes all eukaryotic genomes. Genomic regions within heterochromatin are transcriptionally repressed and aberrant expression can be lethal. Heterochromatin is characterized by specific histone modifications, but reader-repressor proteins are directly responsible for preventing DNA-templated processes. Recent studies suggest immense functional diversity of reader-repressor proteins that remains poorly understood. Understanding this diversity will reveal the ancestral composition and capabilities of eukaryotic heterochromatin and uncover unique heterochromatic systems. While exploring heterochromatin in species across the eukaryotic tree of life is key, most lineages have not been investigated due to complications with chromatin isolation, culturing, and genetic manipulation.
To address these fundamental biological questions and overcome technical barriers, I will use a recently developed mass spectrometry method to identify proteins associated with known heterochromatic modifications in 19 species cultured in the Sebé-Pedrós laboratory representing all major and several early-branching eukaryotic lineages. To overcome genetic manipulation limitations, I will create a synthetic platform to heterologously assess reader-repressor functions of proteins in budding yeast. I will use this system to functionally characterize new regulators of heterochromatin from diverse eukaryotes. Finally, I will integrate these and other genomic data in a comparative framework to reconstruct the ancestral composition and evolutionary diversification of eukaryotic heterochromatin.
Overall, my project will comprehensively identify the functional conservation and diversity of eukaryotic heterochromatin and create a powerful genetic tool to further explore chromatin function and evolution.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2022-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
08003 Barcelona
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.