Objective
Parkinson’s Disease (PD) is the second most common neurodegenerative disease affecting millions world-wide. PD pathophysiology is associated with protein aggregates called Lewy Bodies (LBs), and aggregation is impacted by cellular environment, post-translational modifications and genetic predispositions. Heterogeneity in the composition of LBs across the brain has consequences for neuronal cytotoxicity and although LBs are a main focus in PD research, only a limited number of projects have explored their heterogeneity.
I will investigate LB heterogeneity at the single cell level for the first time -- enabled by technological developments of my host lab, the Mann group, namely Deep Visual Proteomics (DVP). DVP combines high-resolution microscopy, AI-aided image analysis and ultra-sensitive MS-based proteomics to analyse the proteome in a spatially-resolved manner. I aim to identify proteins whose levels correlate with LB heterogeneity, thereby classifying different LB phenotypes and finding ‘tipping points’ in LB maturation that drive cell death.
I will first apply DVP to cell culture models overexpressing alpha-synuclein, a main component of LBs, to investigate the composition of LBs and determine candidates promoting various types of LB phenotypes. Such candidates, e.g. the already known tubulin polymerisation promoting protein (TPPP/p25), will be expressed in cells to confirm effects on the LB phenotype. In parallel, I will apply DVP to human brain tissue to identify differences in aggregates across stages of the disease and to distinguish between healthy and sick cells within the same tissue.
This analysis will provide unique insights into the progression of the disease and may yield new therapeutic candidates.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- medical and health sciences basic medicine physiology pathophysiology
- natural sciences physical sciences optics microscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2023-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.