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Transcription factors and regulation of AQP expression in maize stomatal guard cells and subsidiary cells

Project description

Aquaporins’ roles within stomata to develop stress-resilient crops

Stomata are openings on plant surfaces, formed in grasses by guard and subsidiary cells that allow gas exchange and transpiration. Their regulation involves the movement of water and solutes, facilitated by aquaporins (AQPs), though their precise functions in stomata remain poorly understood. Grass stomata are suitable for studying AQPs under abiotic stress, with maize serving as a key model due to its agronomic importance. Supported by the Marie Skłodowska-Curie Actions programme, the StomAQP project will explore the roles of AQPs and their regulation within stomatal complexes to advance the development of stress-resilient crops. It will use transactivation assays, protoplast engineering, maize suspension cells and CRISPR/Cas technology. The findings could help transfer stress-resilience mechanisms from grasses to other major crops.

Objective

The stomata are openings formed on the epidermis formed by two guard cells (GC) surrounded by subsidiary cells (SC), allowing gaseous exchange for photosynthesis at the expense of water via transpiration. Stomata are tightly regulated by the influx and efflux of water and small solutes across GC or SC membranes. The diffusion of these solutes and water through the membranes can be facilitated by aquaporins (AQPs). While there is insufficient knowledge on the precise role of AQPs in stomata, specific microdissection of the mechanism of AQP action in GCs and SCs affecting stomata is further limited. The superiority of grass stomata owing to their quick response make them apt for studying the role of AQPs in stomatal response, especially under abiotic stress condition since climate change has made crops worldwide extremely vulnerable. In this regard, maize is an appropriate grass crop to use as a model because of its economic importance. The overall goal of this project is to study the role of different AQPs, and their transcriptional regulation in stomatal complexes, that can be used to produce stress-resilient crops. We will be using transactivation assays, protoplast engineering, maize suspension cells, and CRISPR/Cas to achieve the objectives. The mechanisms uncovered here can be monumental to extrapolate the grass stress resilience mechanisms into other vital crops. This project will help add the experience required for my goal of being an independent group leader. I will gain from the scientific environment and the infrastructure and support system at LIBST-UCLouvain as well as from the vast experience of Prof. Chaumont in research and project management. In turn, the host will benefit from the technical skills, and creativity that I can offer.

Fields of science (EuroSciVoc)

Keywords

Programme(s)

Topic(s)

Funding Scheme

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

UNIVERSITE CATHOLIQUE DE LOUVAIN
Net EU contribution
€ 175 920,00
Address
PLACE DE L UNIVERSITE 1
1348 LOUVAIN LA NEUVE
Belgium
Region
Région de Bruxelles-Capitale/Brussels Hoofdstedelijk Gewest Région de Bruxelles-Capitale/ Brussels Hoofdstedelijk Gewest Arr. de Bruxelles-Capitale/Arr. Brussel-Hoofdstad
Activity type
Higher or Secondary Education Establishments
Links
Total cost
No data