Project description
Exploring how cells use protein modification to regulate functions
Cells use a process called ADP-ribosylation to regulate important functions such as responding to stress or damage. This process involves enzymes, including poly-ADP-ribose polymerases that attach small molecules (ADP-ribose) to proteins. While one type of this process (poly-ADP-ribosylation) is known in detail, another type – mono-ADP-ribosylation – is less understood but could be promising for treating immune-related diseases. With the support of the Marie Skłodowska-Curie Actions programme, the mArSTED project will further explore mono-ADP-ribosylation using advanced microscopy to investigate where it happens inside cells. mArSTED will also study how two specific enzymes might work together to control this process across different cell structures. The proposed research will provide unprecedented insights into how cells function.
Objective
ADP-ribosylation (ADPr) is a key post-translational modification catalyzed by the ADP-ribosyltransferase (ART) superfamily, including poly-ADP-ribose polymerases (PARPs), which, despite their name, can transfer either a single (mono-ADPr) or multiple (poly-ADPr; PAR) units of ADP-ribose to their targets. This process occurs in response to various biological and pathological stimuli, regulating numerous cellular functions. For many years, research in the ADPr field focused primarily on poly-ADPr PARPs, often overlooking the physiological importance and therapeutic potential of mono-PARPs. Recently, the development of new reagents, including versatile antibodies to detect mono-ADPr, provided essential tools to finally explore this elusive modification. Emerging evidence, partly obtained by using these antibodies, suggests that mono-ADPr responses could be promising therapeutic targets for immune-related diseases, pushing mono-ADPr into the spotlight. However, this growing pharmaceutical interest contrasts with our limited understanding of the molecular mechanisms and biological functions of mono-ADPr and the catalyzing enzymes. In this MSCA project, I aim to shed light on the functions of the underexplored mono-ADP-ribosylation by pioneering the development of super-resolution microscopy methods to visualize mono-ADPr localization across different cellular structures. Through this cutting-edge approach, my research will provide unprecedented insights into the spatial dynamics of mono-ADPr, potentially uncovering novel aspects of its functional roles in cells and providing powerful new methods for the ADP-ribosylation field. Additionally, I will investigate the crosstalk between two PARPs that, according to preliminary data from the host lab, may collaboratively mediate ADPr at the nuclear pore complex, providing insights into the molecular mechanisms underlying the findings from our microscopy studies.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project's classification has been human-validated.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project's classification has been human-validated.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2024-PF-01
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50931 Koln
Germany
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