Protein free media for cell banks
The use of any human or animal derived media in the manufacture of human therapeutics has been considered potentially unsafe. This is mainly due to the fact that these constituents may affect the final product, mostly in cases where they contain viral agents. For this reason, industrial processes opt for chemically defined media, which do not include any proteins, human, or animal derived elements. This project demonstrated the applicability and the associated economical benefits related to the adoption of protein-free media by current industrial processes. This technology does not require prescreening of human or animal derived lots to the potential contamination of the final purified product. Additionally, it facilitates the easy detection of physiological proteins without any need for separating the potential interfering substances, such as those found in animal media. The project provided the necessary approach for establishing cell-banking systems that are capable of preserving producer cell lines free of any human or animal originated components. The offered result comprises a properly defined cryopreservation and revitalisation method for using protein-free, complete chemically defined culture media. This is essentially a set of protocols for preparing the cell material and generating the cell banks. Using a variety of producer cell lines, such as BHK, CHO and MDCK, the technique has been proven not only applicable, but also reliable. It could ensure optimum cell growth, process reproducibility and easy downstream processing for developing new strategies for the treatment of human diseases.
