Objective
My aim is to dissect the molecular events leading to the differentiation of a specific sub-class of neurons i.e. motoneurons, by using the recently-developed gene trapping technology to identify genes which are specifically transcribed in cells which are differentiating toward a motoneuron phenotype.
1. A replicate bank of embryonic stem cell clones will be generated containing random integrations of the B- galactosidase reporter gene into the mouse genome.
2. Individual ES cell clones will be induced to differentiate in vitro towards a motoneuron phenotype using conditions recently identified in the host laboratory. Clones of interest will be identified by a B-gal assay and further characterised using molecular genetic techniques.
3. These clones will be used to generate transgenic mice in which the in vivo expression pattern of the B-gal (and thus of the trapped gene) as well as the effects on motoneuron development of null mutations in the trapped genes can be analysed.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology cells technologies stem cells
- natural sciences biological sciences genetics mutation
- natural sciences biological sciences genetics genomes
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Coordinator
13288 Marseille
France
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