Objective
From oocytes to neurones and from Drosophila to vertebrates, intracellular targeting and localization of molecules contribute to regulation of gene expression.
In the past year I performed light (lm) and electron (em) microscopic nonisotopic in situ hybridization (ISH) studies to investigate the regional and subcellular distribution of glycine receptor (GlyR) subunit mRNAs in rat and mouse CNS neurones. I found that alpha and beta subunit mRNAs have somatodendritic and somatic localizations, respectively. Studies of other nonneuronal cell types suggest that the differential intracellular distribution of mRNAs depends on signal sequences in the 3'untranslated region (UTR). I will construct, express in neuronal cell cultures, and visualize by lm and em ISH tagged-chimeric GlyR subunit cDNAs, containing or lacking normal or mutated 3'UTR. This will allow identification of the mRNA targeting sequences which distinguish and determine, by interaction with the trafficking machinery, the different intracellular distributions of GlyR subunit transcripts.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75230 PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.