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Content archived on 2024-05-27

Exon trapping insert consortium

Objective

A network of laboratories will be formed to create a large population of gene-trap insertions in the genome of Arabidopsis thaliana to detect the spatial and temporal expression patterns of approximately 5.000 genes, and to identify and characterise the regulatory elements of these genes. Data obtained on the genes, in which transposons are inserted, will be obtained by sequencing the insertion site and this will be related to genome sequence and expression patterns in a functional genomics database. Gene expression patterns in a wide range of tissues and conditions will be defined and put in the database. The products of research will be a powerful resource to analyse the function of 20% of the genes in Arabidopsis, and these will be exploited by industrial partners.
The EXOTIC project has generated 25,367 gene trap lines. This collection stands beside the CSHL collection of 18,764 gene trap and 14,204 enhancer trap lines (http://genetrap.cshl.org/servlet/CurrentStats(opens in new window)). These collections, when available via stock centres and comprehensively described, will be a key asset for understanding gene function and gene expression in Arabidopsis. Table 2 describes the current status of gene disruption in Arabidopsis. The AtIDB database shows that 80% of the 29,000 predicted genes harbour disruptions. This represents outstanding progress towards the community goal of generating disruptions of all genes by 2005. It also shows that EU activities (GABI-KAT, Flag, Garnet and EXOTIC) have contributed nearly half of the lines available for gene disruption. It is likely that the random insertion strategies used with such success so far will not be cost effective in reaching the remaining 20% of genes, so new approaches will be needed. Here transposon insertions, which can be re-mobilised for efficient local mutagenesis, may provide users with resources to tackle genes of interest. Gene traps can also identify gene disruptions in the heterozygous state and that are expressed in the extended haploid stages, which may affect zygote viability. These factors suggest gene trap collections, such as the EXOTIC collection, will provide a key resource for tackling the remaining genes.

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Funding Scheme

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Coordinator

AMICA SCIENCE EEIG
EU contribution
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Address
Norwich Research Park John Innes Centre, Colney
NR4 7UH NORWICH
United Kingdom

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Total cost

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Participants (10)

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