Objective The aim of this proposal is to enhance the European leadership in three-dimensional (3D) electron microscopy. HT-3DEM proposes to develop an automated platform to enable high throughput screening and analysis of native protein complexes and protein crysta ls by electron microscopy. For this purpose, HT-3DEM brings together European experts in electron microscopy, 2D crystallisation, machine vision, and robotics with leading manufacturers of electron microscopes and microsystem technologies. The innovative t echnology platform will include (i) a robot for the production of 2D protein crystals, (ii) the automated preparation of 2D crystals and native protein complexes for EM analysis, (iii) automated image acquisition and sample analysis by electron microscopy and (iv) management of all data produced in the pipeline. Each of these objectives represents a substantial advance of the state of the art. The innovations generated in HT-3DEM will greatly increase the throughput in 3D electron microscopy and thus enhan ce European competitiveness in the field of structural genomics. The reduction of processing times and cost for individual samples will open new fields to the application of this versatile technology platform. These are envisaged to include, among others, health care (screening of patient materials for infective agents) and pharma industry (acceleration of rational drug design). HT-3DEM thus responds to the needs of the European Community to improve human health and to increase the competitiveness of Europ ean pharma industry. Fields of science medical and health sciencesbasic medicinemedicinal chemistrynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencescomputer and information sciencesartificial intelligencecomputer visionnatural sciencesphysical sciencesopticsmicroscopyelectron microscopynatural sciencesbiological sciencesbiochemistrybiomoleculeslipids Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) LSH-2004-1.1.0-1 - Topics for Specific Targeted Research Project in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Call for proposal FP6-2004-LIFESCIHEALTH-5 See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator UNIVERSITAET BASEL EU contribution No data Address Petersgraben 35 BASEL Switzerland See on map Links Website Opens in new window Total cost No data Participants (4) Sort alphabetically Sort by EU Contribution Expand all Collapse all FEI ELECTRON OPTICS B.V. Netherlands EU contribution No data Address Achtseweg Noord 5 EINDHOVEN See on map Links Website Opens in new window Total cost No data MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Germany EU contribution No data Address Hofgartenstrasse 8 MUENCHEN See on map Links Website Opens in new window Total cost No data SEYONIC SA Switzerland EU contribution No data Address Puits-Godet 12 NEUCHÂTEL See on map Links Website Opens in new window Total cost No data UNIVERSITÉ DE HAUTE ALSACE France EU contribution No data Address 2 rue des Frères Lumière MULHOUSE See on map Links Website Opens in new window Total cost No data
