Objectif Hematopoietic stem cells (HSCs) yield blood precursors devoted to unilineage differentiation and production of mature blood cells, including red blood cells, megakaryocytes, lymphocytes and myeloid cells. Precursors of the myeloid lineage differentiate into monocytes and granulocytes but at given stages of their maturity they can be also sources of multinucleated osteoclasts. Consequently, any altered myelopoiesis can be directly linked to deficiencies in osteclastogenesis and impaired bone homeostasis. Retinoic X receptors (RXRa, RXRb and RXRg) are members of the nuclear receptor family. They appear to contribute to hematopoiesis and osteoclastogenesis via their roles as heterodimeric partners of several nuclear receptors. Functionally the most important isotype is RXRa during morphogenesis. Since RXRs can function either as homodimers or heterodimers, it is unclear whether the effects of RXRs on hematopoiesis and osteoclastogenesis are coupled to RXR homodimers, heterodimers, or involve the regulation of parallel pathways. Additionally, in mouse models of RXRa deletion, a partial redundancy in RXRa, b and g functions can be also observed. In this context, the goal of the presented project is to characterize the role of RXRs in HSC differentiation and osteoclastogenesis. In this project we will work with a knockout mouse model in which both RXRa and RXRb are selectively ablated in the hematopoietic system. Using this model we will be able to characterize the in vivo effects of the conditional inhibition of RXRa and b in the murine hematopoietic system and we can clarify the contribution of each isoforms to myeloid cell differentiation and osteoclastogenesis. The results are anticipated to open new perspectives for developing stem cell based therapies for the treatment of bone-related diseases such as osteoporosis or rheumatoid arthritis, nowadays affecting hundreds of millions of people worldwide. Champ scientifique medical and health sciencesclinical medicinerheumatologynatural sciencesbiological sciencescell biologymedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesbasic medicinephysiologyhomeostasis Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2009-RG - Marie Curie Action: "Reintegration Grants" Appel à propositions FP7-PEOPLE-2009-RG Voir d’autres projets de cet appel Régime de financement MC-ERG - European Re-integration Grants (ERG) Coordinateur CENTRO NACIONAL DE INVESTIGACIONES CARDIOVASCULARES CARLOS III (F.S.P.) Contribution de l’UE € 45 000,00 Adresse CALLE MELCHOR FERNANDEZ ALMAGRO 3 28029 Madrid Espagne Voir sur la carte Région Comunidad de Madrid Comunidad de Madrid Madrid Type d’activité Research Organisations Contact administratif Mercedes Ricote (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée