Objective
Stem cell science and technology hold a great deal of promise within the context of tissue repair and regeneration. Whilst we know a lot about the function and response of stem cells in the laboratory, we know very little about their behaviour in tissues within individuals, delaying their applicability to the clinics. A major barrier has been the inability to accurately track cell lineages and to distinguish them from other cell types within the tissue. Fundamental questions, i.e. when one cell divides to become two cells are they the same or different? is this effect the same or different each time the cell divides?, have not been answered yet. The proposed project will address these issues by developing microscopic techniques that allow us to image these cells in a non-invasive, non-harmful way (unlike current approaches) and we will utilise technologies that will eventually enable us to track these cells deep within patient tissues. Coherent Anti-Stokes Raman Scattering (CARS) has recently emerged as a new multiphoton microscopy technique which directly addresses endogenous biomolecules with high chemical specificity without the need for exogenous labelling. This imaging technology would be ideal to follow non-invasively, living stem cells during differentiation in real time at physiological conditions without any of the artefacts/problems associated with labelling. We will use different modalities to identify endogenous markers of stem cell differentiation for tracking purposes. First, CARS and Differential CARS will be used to examine the lipid distributions within stem cells undergoing differentiation. Furthermore, to improve the chemical specificity we will develop a novel Coherent Multiplex CARS method to address a large number of vibrational frequencies simultaneously. In this way tracking of stem/progenitor cells and their progeny in vivo without the need for fluorescent, harmful labels or labels that are lost as the cell divides will be possible.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences optics microscopy
- natural sciences biological sciences biochemistry biomolecules lipids
- medical and health sciences medical biotechnology cells technologies stem cells
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-RG
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CF10 3AT CARDIFF
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.