Designing the next generation of biosensors
Biosensors are increasingly being used in diagnostic devices for the physicochemical detection of biological molecules. The receptor in the device incorporates a component that can interact with a specific molecule of interest, such as antibodies for the detection of proteins. The scope of the EU-funded 'Peptide sensing platforms for proteins and antibodies detection' (PSP) project was to develop an innovative biosensor platform based on proteins for the detection of antibodies. This method would be used at point-of-care to simultaneously detect multiple targets rapidly and conveniently, directly in blood serum. Among the project's objectives was to study the effect of surface on the folding of biomolecules and especially on proteins. For this purpose, the PSP device utilised electrochemistry for monitoring the binding-induced folding of proteins on the sensor. To understand how proteins behave in the presence of a specific antibody, scientists performed optical tests using fluorescently labelled peptides. They also explored the formation of peptide films on different sensor surfaces or even the modification of peptides with redox tags. Attachment of these polypeptides to an electrode surface can be used to detect specific antibodies. The expertise obtained during the PSP study on peptide and polypeptide electrochemical sensors could significantly improve diagnosis, therapies and medical procedures. In addition, this device could be implemented in the routine clinical monitoring of health and wellness.
Keywords
Biosensors, protein, antibodies, point-of-care, blood serum
