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Transition towards environment-friendly consumer products by co-creation of an oxidoreductase foundry

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Risk assessment of developed detergent formulations (opens in new window)

Report on risk assessment for application of new enzymes in detergent formulation, including end-user risk evaluation.

Strategy for BioContainer implementation and modules to develop (opens in new window)

Mapping opportunities for the platform design and their rationale and choice of strategy and timeline for progress

Enzymes produced through optimized fermentation process (opens in new window)

Report on maximum product concentration, yield and productivity of enzymes produced by optimized fermentation in up to two litres.

Social Awareness Report (opens in new window)

Report on consumer awareness on each of the innovation cases, and results of awareness campaigns (e.g. scope, audience range).

Road map on value chain analysis for closed loops (opens in new window)

Report of potential of closed loops of two value-chains.

LCA-assessment of environmental impacts of OXIPRO applications (opens in new window)

Report of complete LCA (ISO 14040-44) on the newly developed enzyme applications (innovation cases), and comparison to reference products.

Validation of the enzyme biotechnology platform (opens in new window)

Testing the screening platform on a list of enzyme candidates to identify its beneficial properties.

Host and expression conditions per enzyme type (opens in new window)

Report on host and expression conditions per enzyme type after lab scale trials and optimization runs

FNR-16 contributions for policymakers (opens in new window)

Minimum of two policy briefs relating to the European Bioeconomy strategy and other relevant strategies,outlining rationales for particular policy alternatives or courses of action we recommend as a result of the findings

Identified optimal cofactor strategy (opens in new window)

Report on results from engineering of cofactor preference and results from enzyme cascades for cofactor regeneration, and which one is preferred as a main strategy.

Industrial scale models of enzyme production. (opens in new window)

Report on kinetic model developed at 1-2L describing growth and enzyme production, and its validation at up to 30L fermentation scale.

Screening systems ready for enzyme tests (opens in new window)

Establishment of biosensory array to monitor enzymatic activity through microfluidics and cellfree expression systems

Work-on data from interviews, round table talks and world cafés - final (opens in new window)

Work-on data from interviews, round table talks and world cafés - final -as an updated / final version of D1.3

E-LCC, S-LCA - Report on social and economic sustainability assessment (opens in new window)

Report on social and economics assessment of each innovation case.

Stakeholder mapping and methodology for stakeholder engagement (opens in new window)

Landscape analysis to identify stakeholders in four key groups Research Business Policy and Society

Scale-up production per industrial application (opens in new window)

Report on production of enzymes by fermentation at up to 30L, including maximum product concentration, yield and productivity.

Work-on data from interviews, round table talks and world cafés (opens in new window)

Information from stakeholders participating in worldcafs and round table talks

Scientific Publication Report (opens in new window)

Report on the scientific publications, including overview of accessibility level, journal, and impact level.

Data modules and first application to 1 OXIPRO enzyme (opens in new window)

First application of a data modules developed in OXIPRO for an enzyme case

Bioprospecting & in silico evolution platform modules (opens in new window)

Established the modules for discovery of new enzymes and engineering of enzymes, targeted for OXIPRO enzymes.

Validation of the enzyme intelligent platform (opens in new window)

Validation of the enzyme modules by academic and industrial partners.

Project website and social media accounts (opens in new window)

Launch of website containing project informaton such as objectives progress news and results and relevant links to sister EU initiatives serving as a main online interface of the OXIPRO project with the different target audiences

OXIPRO videos and dissemination material (opens in new window)

Release of project flyer press releases journalistic articles eNewsletters and two explanatory video 1 one describing the project its objectives and expected impacts aimed at explaining the project focus and 2 a second one aiming at nontechnical target groups to contributing to awareness raising

Publications

Towards a resource friendly circular cotton processing: From carbohydrate rich wastewaters to hydrogen peroxide using carbohydrate oxidases (opens in new window)

Author(s): Yerko Fredes, Gregorio Álvaro, Marina Guillén, Oscar Romero
Published in: Journal of Environmental Chemical Engineering, Issue 13, 2025, Page(s) 115902, ISSN 2213-3437
Publisher: Elsevier BV
DOI: 10.1016/j.jece.2025.115902

Increased Thermostability of an Engineered Flavin-Containing Monooxygenase to Remediate Trimethylamine in Fish Protein Hydrolysates (opens in new window)

Author(s): Marianne Goris, Isabel Cea-Rama, Pål Puntervoll, Rasmus Ree, David Almendral, Julia Sanz-Aparicio, Manuel Ferrer, Gro Elin Kjæreng Bjerga
Published in: Applied and Environmental Microbiology, Issue 89, 2023, ISSN 0099-2240
Publisher: American Society for Microbiology
DOI: 10.1128/aem.00390-23

EP-Pred: A Machine Learning Tool for Bioprospecting Promiscuous Ester Hydrolases (opens in new window)

Author(s): Ruite Xiang, Laura Fernandez-Lopez, Ana Robles-Martín, Manuel Ferrer, Victor Guallar
Published in: Biomolecules, Issue 12, 2025, Page(s) 1529, ISSN 2218-273X
Publisher: MDPI AG
DOI: 10.3390/biom12101529

Activity assays for flavoprotein oxidases: an overview (opens in new window)

Author(s): Lars L. Santema, Marco W. Fraaije
Published in: Applied Microbiology and Biotechnology, Issue 109, 2025, ISSN 1432-0614
Publisher: Springer Science and Business Media LLC
DOI: 10.1007/s00253-025-13494-2

Increased Thermostability of an Engineered Flavin-Containing Monooxygenase to Remediate Trimethylamine in Fish Protein Hydrolysates (opens in new window)

Author(s): Goris, Marianne; Cea-Rama, Isabel; Puntervoll, Pål; Almendral, David; Sanz-Aparicio, Julia; Ferrer, Manuel; Bjerga, Gro Elin Kjæreng
Published in: Applied and Environmental Biology, Issue Vol. 89, No. 6, 2023, ISSN 0099-2240
Publisher: American Society for Microbiology
DOI: 10.1128/aem.00390-23

Discovery and biochemical characterization of thermostable glycerol oxidases (opens in new window)

Author(s): Lars L. Santema, Laura Rotilio, Ruite Xiang, Gwen Tjallinks, Victor Guallar, Andrea Mattevi, Marco W. Fraaije
Published in: Applied Microbiology and Biotechnology, Issue 108, 2025, ISSN 0175-7598
Publisher: Springer Verlag
DOI: 10.1007/s00253-023-12883-9

AsiteDesign: a Semirational Algorithm for an Automated Enzyme Design (opens in new window)

Author(s): Sergi Roda, Henrik Terholsen, Jule Ruth Heike Meyer, Albert Cañellas-Solé, Victor Guallar, Uwe Bornscheuer, Masoud Kazemi
Published in: The Journal of Physical Chemistry B, Issue 127, 2023, Page(s) 2661-2670, ISSN 1520-6106
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcb.2c07091

Ten simple rules on how to develop a stakeholder engagement plan (opens in new window)

Author(s): Susanne Hollmann; Babette Regierer; Jaele Bechis; Lesley Tobin; Domenica D’Elia
Published in: Plos Computational Biology, Issue 12, 2022, ISSN 1553-734X
Publisher: Public Library of Science
DOI: 10.1371/journal.pcbi.1010520

Structural Elucidation and Engineering of a Bacterial Carbohydrate Oxidase (opens in new window)

Author(s): Alessandro Boverio, Wahyu S. Widodo, Lars L. Santema, Henriëtte J. Rozeboom, Ruite Xiang, Víctor Guallar, Andrea Mattevi, Marco W. Fraaije
Published in: Biochemistry, Issue 62, 2023, Page(s) 429-436, ISSN 0006-2960
Publisher: American Chemical Society
DOI: 10.1021/acs.biochem.2c00307

Discovery and structural characterization of a thermostable bacterial monoamine oxidase (opens in new window)

Author(s): Lars L. Santema, Lorenzo Basile, Claudia Binda, Marco W. Fraaije
Published in: The FEBS Journal, Issue 291, 2024, Page(s) 849-864, ISSN 1742-464X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/febs.16973

Identification of a robust bacterial pyranose oxidase that displays an unusual pH dependence (opens in new window)

Author(s): Lars L. Santema, Henriëtte J. Rozeboom, Veronica P. Borger, Saniye G. Kaya, Marco W. Fraaije
Published in: Journal of Biological Chemistry, Issue 300, 2024, Page(s) 107885, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2024.107885

Altering substrate specificity of a thermostable bacterial monoamine oxidase by structure-based mutagenesis (opens in new window)

Author(s): Lorenzo Basile, Chiara Poli, Lars L. Santema, Răzvan C. Lesenciuc, Marco W. Fraaije, Claudia Binda
Published in: Archives of Biochemistry and Biophysics, Issue 764, 2025, Page(s) 110276, ISSN 0003-9861
Publisher: Academic Press
DOI: 10.1016/j.abb.2024.110276

Identification of a robust bacterial pyranose oxidase that displays an unusual pH dependence (opens in new window)

Author(s): Lars L. Santema, Henriëtte J. Rozeboom, Veronica P. Borger, Saniye G. Kaya, Marco W. Fraaije
Published in: Journal of Biological Chemistry, Issue 300, 2024, Page(s) 107885, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2024.107885

Activity assays for flavoprotein oxidases: an overview (opens in new window)

Author(s): Lars L. Santema, Marco W. Fraaije
Published in: Applied Microbiology and Biotechnology, Issue 109, 2025, ISSN 1432-0614
Publisher: Springer Science and Business Media LLC
DOI: 10.1007/s00253-025-13494-2

Towards a resource friendly circular cotton processing: From carbohydrate rich wastewaters to hydrogen peroxide using carbohydrate oxidases (opens in new window)

Author(s): Yerko Fredes, Gregorio Álvaro, Marina Guillén, Oscar Romero
Published in: Journal of Environmental Chemical Engineering, Issue 13, 2025, Page(s) 115902, ISSN 2213-3437
Publisher: Elsevier BV
DOI: 10.1016/j.jece.2025.115902

Design and Characterization of In-One Protease-Esterase PluriZyme (opens in new window)

Author(s): Laura Fernandez-Lopez, Sergi Roda, Jose L. Gonzalez-Alfonso, Francisco J. Plou, Víctor Guallar, Manuel Ferrer
Published in: International Journal of Molecular Sciences, Issue 23, 2025, Page(s) 13337, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232113337

EP-pred: A Machine Learning Tool for Bioprospecting Promiscuous Ester Hydrolases (opens in new window)

Author(s): Xiang, R; Fernandez-Lopez, Laura; Robles-Martín, Ana; Ferrer, Manuel; Guallar, Víctor
Published in: Biomolecules; Volume 12; Issue 10; Pages: 1529, Issue 12, 2022, ISSN 2218-273X
Publisher: Multidisciplinary Digital Publishing Institute
DOI: 10.20944/preprints202209.0100.v1

Biochemical and Structural Characterisation of a Bacterial Lactoperoxidase (opens in new window)

Author(s): Pećanac, Ognjen; Martin, Caterina; Savino, Simone; Rozeboom, Henriette; Fraaije, Marco W.; Lončar, Nikola
Published in: ChemBioChem, 2024, ISSN 1439-7633
Publisher: Wiley-VCH
DOI: 10.5281/zenodo.17689238

Biochemical and Structural Characterisation of a Bacterial Lactoperoxidase (opens in new window)

Author(s): Ognjen Pećanac, Caterina Martin, Simone Savino, Henriette J. Rozeboom, Marco W. Fraaije, Nikola Lončar
Published in: ChemBioChem, Issue 26, 2025, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202400713

Biochemical and Structural Characterisation of a Bacterial Lactoperoxidase (opens in new window)

Author(s): Ognjen Pećanac, Caterina Martin, Simone Savino, Henriette J. Rozeboom, Marco W. Fraaije, Nikola Lončar
Published in: ChemBioChem, 2024, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202400713

Structural Elucidation and Engineering of a Bacterial Carbohydrate Oxidase (opens in new window)

Author(s): Alessandro Boverio; Wahyu S. Widodo; Lars L. Santema; Henriëtte J. Rozeboom; Ruite Xiang; Víctor Guallar; Andrea Mattevi; Marco W. Fraaije
Published in: Biochemistry, Issue 62, 2022, ISSN 1520-4995
Publisher: ACS
DOI: 10.1021/acs.biochem.2c00307

Discovery and biochemical characterization of thermostable glycerol oxidases (opens in new window)

Author(s): Lars L Santema, Laura Rotilio, Ruite Xiang, Gwen Tjallinks, Victor Guallar, Andrea Mattevi, Marco W Fraaije
Published in: Applied microbiology and biotechnology, Issue Mar;291(5), 2024, Page(s) 849-864, ISSN 1432-0614
Publisher: Springer International, c1984-
DOI: 10.1007/s00253-023-12883-9

AsiteDesign: a Semirational Algorithm for an Automated Enzyme Design (opens in new window)

Author(s): Roda, Sergi; Terholsen, Henrik; Heike Meyer, Jule Ruth; Cañellas-Solé, Albert; Guallar, Víctor; Bornscheuer, Uwe; Kazemi, Masoud
Published in: The Journal of Physical Chemistry B, Issue 127, 12,, 2023, Page(s) 2661–2670, ISSN 1520-6106
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcb.2c07091

Altering substrate specificity of a thermostable bacterial monoamine oxidase by structure-based mutagenesis (opens in new window)

Author(s): Lorenzo Basile, Chiara Poli, Lars L. Santema, Răzvan C. Lesenciuc, Marco W. Fraaije, Claudia Binda
Published in: Archives of Biochemistry and Biophysics, Issue 764, 2025, Page(s) 110276, ISSN 0003-9861
Publisher: Academic Press
DOI: 10.1016/j.abb.2024.110276

Biocatalytic application and structural elucidation of robust bacterial protein nanocages (opens in new window)

Author(s): Ognjen Pećanac, Alexander Belyy, Caterina Martin, Rosalie Kleissen, Marco W. Fraaije, Nikola Lončar
Published in: Materials Advances, Issue 6, 2025, Page(s) 5303-5309, ISSN 2633-5409
Publisher: Royal Society of Chemistry (RSC)
DOI: 10.1039/d5ma00268k

Design and Characterization of In-One Protease-Esterase PluriZyme (opens in new window)

Author(s): Laura Fernandez-Lopez; Sergi Roda; Jose L. Gonzalez-Alfonso; Francisco J. Plou; Víctor Guallar; Manuel Ferrer
Published in: International Journal of Molecular Sciences, Issue 23, 2022, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232113337

Ten simple rules on how to develop a stakeholder engagement plan (opens in new window)

Author(s): Susanne Hollmann, Babette Regierer, Jaele Bechis, Lesley Tobin, Domenica D’Elia
Published in: PLOS Computational Biology, Issue 18, 2022, Page(s) e1010520, ISSN 1553-7358
Publisher: Public Library of Science (PLoS)
DOI: 10.1371/journal.pcbi.1010520

Discovery and structural characterization of a thermostable bacterial monoamine oxidase (opens in new window)

Author(s): Lars L Santema, Lorenzo Basile, Claudia Binda, Marco W Fraaije
Published in: FEBS Journal Pub Med, Issue Mar;291(5):849-864, 2024, Page(s) 849-864, ISSN 1742-4658
Publisher: Wiley
DOI: 10.1111/febs.16973

Trimethylamine removal in salmon protein hydrolysates by a novel monooxygenase strategy (opens in new window)

Author(s): Goris, Marianne; Puntervoll, Pål; Almendral, David; Ferrer, Manuel; Bjerga, Gro Elin Kjæreng
Published in: Issue 1, 2022
Publisher: Zenodo
DOI: 10.5281/zenodo.7977355

Enzymes for Greener Products (FNR-16 Cluster) Policy Brief (opens in new window)

Author(s): Tobin, L., Berga, G., Ferrer, M., Hidalgo, A., Peñalva, C., Molina, P., Müller, M., Weber, R., Hollmann, S., Dominguez, P., Klement, T., Nejda, N., & Picinetti, L.
Published in: 2023
Publisher: CERN
DOI: 10.5281/zenodo.7745318

OXIPRO: Harnessing the Power of Enzymes for Greener Consumer Products - Case studies factsheets. (opens in new window)

Author(s): Aydemir, G., Bassegoda Puigdomenec, A., Besen de Oliveira, B., Bjerga, G., Guillén Montalban, M., Hollmann, S., Regierer, B., Rey Campa, A., & Tobin, L. (2023).
Published in: 2023
Publisher: zenodo
DOI: 10.5281/zenodo.7866666

Novel oxidases for in situ production of antimicrobial molecules in laundry (opens in new window)

Author(s): Balfagon Campos, Helena; Bassegoda, Arnau; Rey Campa, Aroa; Fraaije, Marco; Santema, Lars
Published in: Issue 7, 2022
Publisher: ZENODO
DOI: 10.5281/zenodo.11924453

Oxidases for in situ production of antimicrobial molecules in laundry

Author(s): Rey Campa, Aroa; Bassegoda, Arnau; Balfagon Campos, Helena; Kotsakis, Panos; Santema, Lars; Fraaije, Marco
Published in: Issue 3, 2023
Publisher: ZENODO

Voices for a Sustainable Bio-based Future: Insights from the World Café Dialogues within the FNR-16 sister projects (opens in new window)

Author(s): Hollmann, Susanne; Hollmann, Christiane; Bjerga, Gro; Tobin, Lesley
Published in: Issue 6, 2025
Publisher: zenodo
DOI: 10.5281/zenodo.15489425

OXIPRO: Harnessing the Power of Enzymes for Greener Consumer Products - Case studies factsheets. (opens in new window)

Author(s): Aydemir, G., Bassegoda Puigdomenec, A., Besen de Oliveira, B., Bjerga, G., Guillén Montalban, M., Hollmann, S., Regierer, B., Rey Campa, A., & Tobin, L. (2023).
Published in: 2023
Publisher: ZENODO
DOI: 10.5281/zenodo.7866666

In silico bioprospecting and engineering laccases: Methods and applications (opens in new window)

Author(s): Ruite Xiang, Martin Floor, Lars H. Østergaard, Kirk M. Schnorr, Victor Guallar
Published in: Laccase and Polyphenol Oxidase, 2025, Page(s) 247-268, ISBN 9780443133015
Publisher: Elsevier
DOI: 10.1016/b978-0-443-13301-5.00009-3

In silico bioprospecting and engineering laccases: Methods and applications (opens in new window)

Author(s): Ruite Xiang, Martin Floor, Lars H. Østergaard, Kirk M. Schnorr, Victor Guallar
Published in: Laccase and Polyphenol Oxidase, 2025, Page(s) 247-268
Publisher: Elsevier
DOI: 10.1016/b978-0-443-13301-5.00009-3

Measuring sustainability: life cycle assessments drive green innovation in OXIPRO (opens in new window)

Author(s): Gro Bjerga, Lesley Tobin, Katri Behm, Inka-Mari Sarvola, Mona Arnold
Published in: The Project Repository Journal, Issue 23, 2025, Page(s) 26-29, ISSN 2632-4067
Publisher: European Dissemination Media Agency Limited
DOI: 10.54050/prj23250

Sustainable UV filters production through laccase assisted oxidative polymerization (opens in new window)

Author(s): Balfagón, Helena; Bassegoda, Arnau; Rey, Aroa; Randelli, Giuseppe; Rando, Pietro
Published in: Issue 3, 2023
Publisher: ZENODO
DOI: 10.5281/zenodo.10069228

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