Transition towards environment-friendly consumer products by co-creation of an oxidoreductase foundry

Informazioni relative al progetto

OXIPRO

ID dell’accordo di sovvenzione: 101000607

DOI

10.3030/101000607

Progetto chiuso

Data della firma CE 5 Maggio 2021

Data di avvio 1 Giugno 2021

Data di completamento 31 Maggio 2025

Finanziato da

• SOCIETAL CHALLENGES - Food security, sustainable agriculture and forestry, marine, maritime and inland water research, and the bioeconomy

Costo totale

€ 5 999 775,00

Contributo UE

€ 5 999 775,00

Coordinato da

NORCE RESEARCH AS
Norway

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (18)

Risk assessment of developed detergent formulations

Report on risk assessment for application of new enzymes in detergent formulation, including end-user risk evaluation.

Strategy for BioContainer implementation and modules to develop

Mapping opportunities for the platform design and their rationale and choice of strategy and timeline for progress

Enzymes produced through optimized fermentation process

Report on maximum product concentration, yield and productivity of enzymes produced by optimized fermentation in up to two litres.

Social Awareness Report

Report on consumer awareness on each of the innovation cases, and results of awareness campaigns (e.g. scope, audience range).

Road map on value chain analysis for closed loops

Report of potential of closed loops of two value-chains.

LCA-assessment of environmental impacts of OXIPRO applications

Report of complete LCA (ISO 14040-44) on the newly developed enzyme applications (innovation cases), and comparison to reference products.

Validation of the enzyme biotechnology platform

Testing the screening platform on a list of enzyme candidates to identify its beneficial properties.

Host and expression conditions per enzyme type

Report on host and expression conditions per enzyme type after lab scale trials and optimization runs

FNR-16 contributions for policymakers

Minimum of two policy briefs relating to the European Bioeconomy strategy and other relevant strategies,outlining rationales for particular policy alternatives or courses of action we recommend as a result of the findings

Identified optimal cofactor strategy

Report on results from engineering of cofactor preference and results from enzyme cascades for cofactor regeneration, and which one is preferred as a main strategy.

Industrial scale models of enzyme production.

Report on kinetic model developed at 1-2L describing growth and enzyme production, and its validation at up to 30L fermentation scale.

Screening systems ready for enzyme tests

Establishment of biosensory array to monitor enzymatic activity through microfluidics and cellfree expression systems

Work-on data from interviews, round table talks and world cafés - final

Work-on data from interviews, round table talks and world cafés - final -as an updated / final version of D1.3

E-LCC, S-LCA - Report on social and economic sustainability assessment

Report on social and economics assessment of each innovation case.

Stakeholder mapping and methodology for stakeholder engagement

Landscape analysis to identify stakeholders in four key groups Research Business Policy and Society

Scale-up production per industrial application

Report on production of enzymes by fermentation at up to 30L, including maximum product concentration, yield and productivity.

Work-on data from interviews, round table talks and world cafés

Information from stakeholders participating in worldcafs and round table talks

Scientific Publication Report

Report on the scientific publications, including overview of accessibility level, journal, and impact level.

Other (3)

Data modules and first application to 1 OXIPRO enzyme

First application of a data modules developed in OXIPRO for an enzyme case

Bioprospecting & in silico evolution platform modules

Established the modules for discovery of new enzymes and engineering of enzymes, targeted for OXIPRO enzymes.

Validation of the enzyme intelligent platform

Validation of the enzyme modules by academic and industrial partners.

Websites, patent fillings, videos etc. (2)

Project website and social media accounts

Launch of website containing project informaton such as objectives progress news and results and relevant links to sister EU initiatives serving as a main online interface of the OXIPRO project with the different target audiences

OXIPRO videos and dissemination material

Release of project flyer press releases journalistic articles eNewsletters and two explanatory video 1 one describing the project its objectives and expected impacts aimed at explaining the project focus and 2 a second one aiming at nontechnical target groups to contributing to awareness raising

Pubblicazioni

Peer reviewed articles (28)

Towards a resource friendly circular cotton processing: From carbohydrate rich wastewaters to hydrogen peroxide using carbohydrate oxidases

Autori: Yerko Fredes, Gregorio Álvaro, Marina Guillén, Oscar Romero
Pubblicato in: Journal of Environmental Chemical Engineering, Numero 13, 2025, Pagina/e 115902, ISSN 2213-3437
Editore: Elsevier BV
DOI: 10.1016/j.jece.2025.115902

Increased Thermostability of an Engineered Flavin-Containing Monooxygenase to Remediate Trimethylamine in Fish Protein Hydrolysates

Autori: Marianne Goris, Isabel Cea-Rama, Pål Puntervoll, Rasmus Ree, David Almendral, Julia Sanz-Aparicio, Manuel Ferrer, Gro Elin Kjæreng Bjerga
Pubblicato in: Applied and Environmental Microbiology, Numero 89, 2023, ISSN 0099-2240
Editore: American Society for Microbiology
DOI: 10.1128/aem.00390-23

EP-Pred: A Machine Learning Tool for Bioprospecting Promiscuous Ester Hydrolases

Autori: Ruite Xiang, Laura Fernandez-Lopez, Ana Robles-Martín, Manuel Ferrer, Victor Guallar
Pubblicato in: Biomolecules, Numero 12, 2025, Pagina/e 1529, ISSN 2218-273X
Editore: MDPI AG
DOI: 10.3390/biom12101529

Activity assays for flavoprotein oxidases: an overview

Autori: Lars L. Santema, Marco W. Fraaije
Pubblicato in: Applied Microbiology and Biotechnology, Numero 109, 2025, ISSN 1432-0614
Editore: Springer Science and Business Media LLC
DOI: 10.1007/s00253-025-13494-2

Increased Thermostability of an Engineered Flavin-Containing Monooxygenase to Remediate Trimethylamine in Fish Protein Hydrolysates

Autori: Goris, Marianne; Cea-Rama, Isabel; Puntervoll, Pål; Almendral, David; Sanz-Aparicio, Julia; Ferrer, Manuel; Bjerga, Gro Elin Kjæreng
Pubblicato in: Applied and Environmental Biology, Numero Vol. 89, No. 6, 2023, ISSN 0099-2240
Editore: American Society for Microbiology
DOI: 10.1128/aem.00390-23

Discovery and biochemical characterization of thermostable glycerol oxidases

Autori: Lars L. Santema, Laura Rotilio, Ruite Xiang, Gwen Tjallinks, Victor Guallar, Andrea Mattevi, Marco W. Fraaije
Pubblicato in: Applied Microbiology and Biotechnology, Numero 108, 2025, ISSN 0175-7598
Editore: Springer Verlag
DOI: 10.1007/s00253-023-12883-9

AsiteDesign: a Semirational Algorithm for an Automated Enzyme Design

Autori: Sergi Roda, Henrik Terholsen, Jule Ruth Heike Meyer, Albert Cañellas-Solé, Victor Guallar, Uwe Bornscheuer, Masoud Kazemi
Pubblicato in: The Journal of Physical Chemistry B, Numero 127, 2023, Pagina/e 2661-2670, ISSN 1520-6106
Editore: American Chemical Society
DOI: 10.1021/acs.jpcb.2c07091

Ten simple rules on how to develop a stakeholder engagement plan

Autori: Susanne Hollmann; Babette Regierer; Jaele Bechis; Lesley Tobin; Domenica D’Elia
Pubblicato in: Plos Computational Biology, Numero 12, 2022, ISSN 1553-734X
Editore: Public Library of Science
DOI: 10.1371/journal.pcbi.1010520

Structural Elucidation and Engineering of a Bacterial Carbohydrate Oxidase

Autori: Alessandro Boverio, Wahyu S. Widodo, Lars L. Santema, Henriëtte J. Rozeboom, Ruite Xiang, Víctor Guallar, Andrea Mattevi, Marco W. Fraaije
Pubblicato in: Biochemistry, Numero 62, 2023, Pagina/e 429-436, ISSN 0006-2960
Editore: American Chemical Society
DOI: 10.1021/acs.biochem.2c00307

Discovery and structural characterization of a thermostable bacterial monoamine oxidase

Autori: Lars L. Santema, Lorenzo Basile, Claudia Binda, Marco W. Fraaije
Pubblicato in: The FEBS Journal, Numero 291, 2024, Pagina/e 849-864, ISSN 1742-464X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/febs.16973

Identification of a robust bacterial pyranose oxidase that displays an unusual pH dependence

Autori: Lars L. Santema, Henriëtte J. Rozeboom, Veronica P. Borger, Saniye G. Kaya, Marco W. Fraaije
Pubblicato in: Journal of Biological Chemistry, Numero 300, 2024, Pagina/e 107885, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2024.107885

Altering substrate specificity of a thermostable bacterial monoamine oxidase by structure-based mutagenesis

Autori: Lorenzo Basile, Chiara Poli, Lars L. Santema, Răzvan C. Lesenciuc, Marco W. Fraaije, Claudia Binda
Pubblicato in: Archives of Biochemistry and Biophysics, Numero 764, 2025, Pagina/e 110276, ISSN 0003-9861
Editore: Academic Press
DOI: 10.1016/j.abb.2024.110276

Identification of a robust bacterial pyranose oxidase that displays an unusual pH dependence

Autori: Lars L. Santema, Henriëtte J. Rozeboom, Veronica P. Borger, Saniye G. Kaya, Marco W. Fraaije
Pubblicato in: Journal of Biological Chemistry, Numero 300, 2024, Pagina/e 107885, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2024.107885

Activity assays for flavoprotein oxidases: an overview

Autori: Lars L. Santema, Marco W. Fraaije
Pubblicato in: Applied Microbiology and Biotechnology, Numero 109, 2025, ISSN 1432-0614
Editore: Springer Science and Business Media LLC
DOI: 10.1007/s00253-025-13494-2

Towards a resource friendly circular cotton processing: From carbohydrate rich wastewaters to hydrogen peroxide using carbohydrate oxidases

Autori: Yerko Fredes, Gregorio Álvaro, Marina Guillén, Oscar Romero
Pubblicato in: Journal of Environmental Chemical Engineering, Numero 13, 2025, Pagina/e 115902, ISSN 2213-3437
Editore: Elsevier BV
DOI: 10.1016/j.jece.2025.115902

Design and Characterization of In-One Protease-Esterase PluriZyme

Autori: Laura Fernandez-Lopez, Sergi Roda, Jose L. Gonzalez-Alfonso, Francisco J. Plou, Víctor Guallar, Manuel Ferrer
Pubblicato in: International Journal of Molecular Sciences, Numero 23, 2025, Pagina/e 13337, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232113337

EP-pred: A Machine Learning Tool for Bioprospecting Promiscuous Ester Hydrolases

Autori: Xiang, R; Fernandez-Lopez, Laura; Robles-Martín, Ana; Ferrer, Manuel; Guallar, Víctor
Pubblicato in: Biomolecules; Volume 12; Numero 10; Pages: 1529, Numero 12, 2022, ISSN 2218-273X
Editore: Multidisciplinary Digital Publishing Institute
DOI: 10.20944/preprints202209.0100.v1

Biochemical and Structural Characterisation of a Bacterial Lactoperoxidase

Autori: Pećanac, Ognjen; Martin, Caterina; Savino, Simone; Rozeboom, Henriette; Fraaije, Marco W.; Lončar, Nikola
Pubblicato in: ChemBioChem, 2024, ISSN 1439-7633
Editore: Wiley-VCH
DOI: 10.5281/zenodo.17689238

Biochemical and Structural Characterisation of a Bacterial Lactoperoxidase

Autori: Ognjen Pećanac, Caterina Martin, Simone Savino, Henriette J. Rozeboom, Marco W. Fraaije, Nikola Lončar
Pubblicato in: ChemBioChem, Numero 26, 2025, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202400713

Biochemical and Structural Characterisation of a Bacterial Lactoperoxidase

Autori: Ognjen Pećanac, Caterina Martin, Simone Savino, Henriette J. Rozeboom, Marco W. Fraaije, Nikola Lončar
Pubblicato in: ChemBioChem, 2024, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202400713

Structural Elucidation and Engineering of a Bacterial Carbohydrate Oxidase

Autori: Alessandro Boverio; Wahyu S. Widodo; Lars L. Santema; Henriëtte J. Rozeboom; Ruite Xiang; Víctor Guallar; Andrea Mattevi; Marco W. Fraaije
Pubblicato in: Biochemistry, Numero 62, 2022, ISSN 1520-4995
Editore: ACS
DOI: 10.1021/acs.biochem.2c00307

Discovery and biochemical characterization of thermostable glycerol oxidases

Autori: Lars L Santema, Laura Rotilio, Ruite Xiang, Gwen Tjallinks, Victor Guallar, Andrea Mattevi, Marco W Fraaije
Pubblicato in: Applied microbiology and biotechnology, Numero Mar;291(5), 2024, Pagina/e 849-864, ISSN 1432-0614
Editore: Springer International, c1984-
DOI: 10.1007/s00253-023-12883-9

AsiteDesign: a Semirational Algorithm for an Automated Enzyme Design

Autori: Roda, Sergi; Terholsen, Henrik; Heike Meyer, Jule Ruth; Cañellas-Solé, Albert; Guallar, Víctor; Bornscheuer, Uwe; Kazemi, Masoud
Pubblicato in: The Journal of Physical Chemistry B, Numero 127, 12,, 2023, Pagina/e 2661–2670, ISSN 1520-6106
Editore: American Chemical Society
DOI: 10.1021/acs.jpcb.2c07091

Altering substrate specificity of a thermostable bacterial monoamine oxidase by structure-based mutagenesis

Autori: Lorenzo Basile, Chiara Poli, Lars L. Santema, Răzvan C. Lesenciuc, Marco W. Fraaije, Claudia Binda
Pubblicato in: Archives of Biochemistry and Biophysics, Numero 764, 2025, Pagina/e 110276, ISSN 0003-9861
Editore: Academic Press
DOI: 10.1016/j.abb.2024.110276

Biocatalytic application and structural elucidation of robust bacterial protein nanocages

Autori: Ognjen Pećanac, Alexander Belyy, Caterina Martin, Rosalie Kleissen, Marco W. Fraaije, Nikola Lončar
Pubblicato in: Materials Advances, Numero 6, 2025, Pagina/e 5303-5309, ISSN 2633-5409
Editore: Royal Society of Chemistry (RSC)
DOI: 10.1039/d5ma00268k

Design and Characterization of In-One Protease-Esterase PluriZyme

Autori: Laura Fernandez-Lopez; Sergi Roda; Jose L. Gonzalez-Alfonso; Francisco J. Plou; Víctor Guallar; Manuel Ferrer
Pubblicato in: International Journal of Molecular Sciences, Numero 23, 2022, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232113337

Ten simple rules on how to develop a stakeholder engagement plan

Autori: Susanne Hollmann, Babette Regierer, Jaele Bechis, Lesley Tobin, Domenica D’Elia
Pubblicato in: PLOS Computational Biology, Numero 18, 2022, Pagina/e e1010520, ISSN 1553-7358
Editore: Public Library of Science (PLoS)
DOI: 10.1371/journal.pcbi.1010520

Discovery and structural characterization of a thermostable bacterial monoamine oxidase

Autori: Lars L Santema, Lorenzo Basile, Claudia Binda, Marco W Fraaije
Pubblicato in: FEBS Journal Pub Med, Numero Mar;291(5):849-864, 2024, Pagina/e 849-864, ISSN 1742-4658
Editore: Wiley
DOI: 10.1111/febs.16973

Other (7)

Trimethylamine removal in salmon protein hydrolysates by a novel monooxygenase strategy

Autori: Goris, Marianne; Puntervoll, Pål; Almendral, David; Ferrer, Manuel; Bjerga, Gro Elin Kjæreng
Pubblicato in: Numero 1, 2022
Editore: Zenodo
DOI: 10.5281/zenodo.7977355

Enzymes for Greener Products (FNR-16 Cluster) Policy Brief

Autori: Tobin, L., Berga, G., Ferrer, M., Hidalgo, A., Peñalva, C., Molina, P., Müller, M., Weber, R., Hollmann, S., Dominguez, P., Klement, T., Nejda, N., & Picinetti, L.
Pubblicato in: 2023
Editore: CERN
DOI: 10.5281/zenodo.7745318

OXIPRO: Harnessing the Power of Enzymes for Greener Consumer Products - Case studies factsheets.

Autori: Aydemir, G., Bassegoda Puigdomenec, A., Besen de Oliveira, B., Bjerga, G., Guillén Montalban, M., Hollmann, S., Regierer, B., Rey Campa, A., & Tobin, L. (2023).
Pubblicato in: 2023
Editore: zenodo
DOI: 10.5281/zenodo.7866666

Novel oxidases for in situ production of antimicrobial molecules in laundry

Autori: Balfagon Campos, Helena; Bassegoda, Arnau; Rey Campa, Aroa; Fraaije, Marco; Santema, Lars
Pubblicato in: Numero 7, 2022
Editore: ZENODO
DOI: 10.5281/zenodo.11924453

Oxidases for in situ production of antimicrobial molecules in laundry

Autori: Rey Campa, Aroa; Bassegoda, Arnau; Balfagon Campos, Helena; Kotsakis, Panos; Santema, Lars; Fraaije, Marco
Pubblicato in: Numero 3, 2023
Editore: ZENODO

Voices for a Sustainable Bio-based Future: Insights from the World Café Dialogues within the FNR-16 sister projects

Autori: Hollmann, Susanne; Hollmann, Christiane; Bjerga, Gro; Tobin, Lesley
Pubblicato in: Numero 6, 2025
Editore: zenodo
DOI: 10.5281/zenodo.15489425

OXIPRO: Harnessing the Power of Enzymes for Greener Consumer Products - Case studies factsheets.

Autori: Aydemir, G., Bassegoda Puigdomenec, A., Besen de Oliveira, B., Bjerga, G., Guillén Montalban, M., Hollmann, S., Regierer, B., Rey Campa, A., & Tobin, L. (2023).
Pubblicato in: 2023
Editore: ZENODO
DOI: 10.5281/zenodo.7866666

Book chapters (2)

In silico bioprospecting and engineering laccases: Methods and applications

Autori: Ruite Xiang, Martin Floor, Lars H. Østergaard, Kirk M. Schnorr, Victor Guallar
Pubblicato in: Laccase and Polyphenol Oxidase, 2025, Pagina/e 247-268, ISBN 9780443133015
Editore: Elsevier
DOI: 10.1016/b978-0-443-13301-5.00009-3

In silico bioprospecting and engineering laccases: Methods and applications

Autori: Ruite Xiang, Martin Floor, Lars H. Østergaard, Kirk M. Schnorr, Victor Guallar
Pubblicato in: Laccase and Polyphenol Oxidase, 2025, Pagina/e 247-268
Editore: Elsevier
DOI: 10.1016/b978-0-443-13301-5.00009-3

Non-peer reviewed articles (1)

Measuring sustainability: life cycle assessments drive green innovation in OXIPRO

Autori: Gro Bjerga, Lesley Tobin, Katri Behm, Inka-Mari Sarvola, Mona Arnold
Pubblicato in: The Project Repository Journal, Numero 23, 2025, Pagina/e 26-29, ISSN 2632-4067
Editore: European Dissemination Media Agency Limited
DOI: 10.54050/prj23250

Conference proceedings (1)

Sustainable UV filters production through laccase assisted oxidative polymerization

Autori: Balfagón, Helena; Bassegoda, Arnau; Rey, Aroa; Randelli, Giuseppe; Rando, Pietro
Pubblicato in: Numero 3, 2023
Editore: ZENODO
DOI: 10.5281/zenodo.10069228