Deciphering the role of RNA modifications during ribosomal decoding and protein synthesis

Project Information

tRNAslation

Grant agreement ID: 101001394

DOI

10.3030/101001394

EC signature date 2 April 2021

Start date 1 June 2021

End date 30 November 2026

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 997 500,00

EU contribution

€ 1 997 500,00

Coordinated by

UNIWERSYTET JAGIELLONSKI
Poland

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (26)

Determining the effects of pseudouridine incorporation on human tRNAs

Author(s): Anna D Biela, Jakub S Nowak, Artur P Biela, Sunandan Mukherjee, Seyed Naeim Moafinejad, Satyabrata Maiti, Andrzej Chramiec-Głąbik, Rahul Mehta, Jakub Jeżowski, Dominika Dobosz, Priyanka Dahate, Veronique Arluison, Frank Wien, Paulina Indyka, Michal Rawski, Janusz M Bujnicki, Ting-Yu Lin, Sebastian Glatt
Published in: The EMBO Journal, Issue 44, 2025, Page(s) 3553-3585, ISSN 1460-2075
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s44318-025-00443-y

Functional divergence of the two Elongator subcomplexes during neurodevelopment

Author(s): Monika Gaik; Marija Kojic; Megan R Stegeman; Tülay Öncü‐Öner; Anna Kościelniak; Alun Jones; Ahmed Mohamed; Pak Yan Stefanie Chau; Sazia Sharmin; Andrzej Chramiec‐Głąbik; Paulina Indyka; Michał Rawski; Anna Biela; Dominika Dobosz; Amanda Millar; Vann Chau; Aycan Ünalp; Michael Piper; Mark C Bellingham; Evan E Eichler; Deborah A Nickerson; Handan Güleryüz; Nour El Hana Abbassi; Konrad
Published in: EMBO Molecular Medicine, Issue Volume 14, Issue 7, 2022, ISSN 1757-4676
Publisher: John Wiley & Sons Ltd.
DOI: 10.15252/emmm.202115608

It takes two to tRNAgo

Author(s): Igor Kaczmarczyk; Łukasz Koziej; Sebastian Glatt
Published in: Structure, Issue Volume 32, Issue 3, 2024, ISSN 0969-2126
Publisher: Cell Press
DOI: 10.1016/j.str.2024.02.005

Pseudouridines in RNAs: switching atoms means shifting paradigms.

Author(s): Ting-Yu Lin; Rahul Mehta; Sebastian Glatt
Published in: FEBS Letters, Issue 595, 2021, ISSN 1873-3468
Publisher: John Wiley & Sons Ltd
DOI: 10.1002/1873-3468.14188

Structural and biochemical characterization of the 3′-5′ tRNA splicing ligases

Author(s): Sebastian Chamera, Weronika Zajko, Mariusz Czarnocki-Cieciura, Marcin Jaciuk, Łukasz Koziej, Jakub Nowak, Krzysztof Wycisk, Małgorzata Sroka, Andrzej Chramiec-Głąbik, Mirosław Śmietański, Filip Gołębiowski, Marcin Warmiński, Jacek Jemielity, Sebastian Glatt, Marcin Nowotny
Published in: Journal of Biological Chemistry, Issue 301, 2025, Page(s) 108506, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2025.108506

Autophosphorylation of conserved yeast and human casein kinase 1 isozymes regulates Elongator-dependent tRNA modifications

Author(s): Maria Friederike Landrock, Rościsław Krutyhołowa, Pauline Böhnert, Jarosław Mazur, Małgorzata Honc, Alexander Hammermeister, Larissa Bessler, David Scherf, Anna Elms, Natalia Radczuk, Bozena Skupien-Rabian, Urszula Jankowska, Friedrich W Herberg, Mark Helm, Roland Klassen, Sebastian Glatt, Raffael Schaffrath
Published in: Nucleic Acids Research, Issue 53, 2025, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaf881

Mapping rRNA, tRNA, and mRNA modifications in ribosomes at high resolution

Author(s): Łukasz Koziej, Sebastian Glatt
Published in: Trends in Biochemical Sciences, Issue 50, 2026, Page(s) 1027-1041, ISSN 0968-0004
Publisher: Elsevier BV
DOI: 10.1016/j.tibs.2025.08.007

tRNA binding to Kti12 is crucial for wobble uridine modification by Elongator

Author(s): David Scherf, Alexander Hammermeister, Pauline Böhnert, Alicia Burkard, Mark Helm, Sebastian Glatt, Raffael Schaffrath
Published in: Nucleic Acids Research, Issue 53, 2025, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaf296

Cryo-EM structure of the fully assembled Elongator complex

Author(s): Marcin Jaciuk; David Scherf; Karol Kaszuba; Monika Gaik; Alexander Rau; Anna Kościelniak; Rościsław Krutyhołowa; Michał Rawski; Paulina Indyka; Andrea Graziadei; Andrzej Chramiec-Głąbik; Anna Biela; Dominika Dobosz; Ting-Yu Lin; Nour-el-Hana Abbassi; Alexander Hammermeister; Juri Rappsilber; Jan Kosinski; Raffael Schaffrath; Sebastian Glatt
Published in: Nucleic Acids Research, 2023, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/nar/gkac1232

Conserved structures and dynamics in 5′-proximal regions of Betacoronavirus RNA genomes

Author(s): Tales Rocha de Moura, Elżbieta Purta, Agata Bernat, Eva M Martín-Cuevas, Małgorzata Kurkowska, Eugene F Baulin, Sunandan Mukherjee, Jakub Nowak, Artur P Biela, Michał Rawski, Sebastian Glatt, Fernando Moreno-Herrero, Janusz M Bujnicki
Published in: Nucleic Acids Research, Issue 52, 2024, Page(s) 3419-3432, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkae144

The molecular basis of tRNA selectivity by human pseudouridine synthase 3

Author(s): Ting-Yu Lin, Leon Kleemann, Jakub Jeżowski, Dominika Dobosz, Michał Rawski, Paulina Indyka, Grzegorz Ważny, Rahul Mehta, Andrzej Chramiec-Głąbik, Łukasz Koziej, Tristan Ranff, Christian Fufezan, Mateusz Wawro, Jakub Kochan, Joanna Bereta, Sebastian A. Leidel, Sebastian Glatt
Published in: Molecular Cell, Issue 84, 2024, Page(s) 2472-2489.e8, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2024.06.013

Mechanistic insight into the pseudouridylation of RNA

Author(s): Ting-Yu Lin, Yasmin Stone, Sebastian Glatt
Published in: RNA Biology, Issue 22, 2025, Page(s) 1-25, ISSN 1547-6286
Publisher: Landes Bioscience
DOI: 10.1080/15476286.2025.2541421

Cryo-EM structures of the human Elongator complex at work

Author(s): Nour-el-Hana Abbassi, Marcin Jaciuk, David Scherf, Pauline Böhnert, Alexander Rau, Alexander Hammermeister, Michał Rawski, Paulina Indyka, Grzegorz Wazny, Andrzej Chramiec-Głąbik, Dominika Dobosz, Bozena Skupien-Rabian, Urszula Jankowska, Juri Rappsilber, Raffael Schaffrath, Ting-Yu Lin, Sebastian Glatt
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-48251-y

Elongator and the role of its subcomplexes in human diseases

Author(s): Monika Gaik; Marija Kojic; Brandon J Wainwright; Sebastian Glatt
Published in: EMBO Molecular Medicine, Issue 1, 2022, Page(s) 1757-4676, ISSN 1757-4676
Publisher: John Wiley & Sons Ltd.
DOI: 10.15252/emmm.202216418

Destabilization of mutated human PUS3 protein causes intellectual disability

Author(s): Ting-Yu Lin, Robert Smigiel, Bozena Kuzniewska, Joanna J. Chmielewska, Joanna Kosińska, Mateusz Biela, Anna Biela, Anna Kościelniak, Dominika Dobosz, Izabela Laczmanska, Andrzej Chramiec-Głąbik, Jakub Jeżowski, Jakub Nowak, Monika Gos, Sylwia Rzonca-Niewczas, Magdalena Dziembowska, Rafał Ploski, Sebastian Glatt
Published in: Human Mutation, 2022, Page(s) 2063-2078, ISSN 1098-1004
Publisher: John Wiley & Sons
DOI: 10.1002/humu.24471

A novel ELP1 mutation impairs the function of the Elongator complex and causes a severe neurodevelopmental phenotype

Author(s): Marija Kojic, Nour E. H. Abbassi, Ting-Yu Lin, Alun Jones, Emma L. Wakeling, Emma Clement, Vasiliki Nakou, Matthew Singleton, Dominika Dobosz, Marios Kaliakatsos, Sebastian Glatt & Brandon J. Wainwright
Published in: J Hum Genet, 2023, Page(s) 445-453, ISSN 1434-5161
Publisher: Springer Verlag
DOI: 10.1038/s10038-023-01135-3

The diverse structural modes of tRNA binding and recognition

Author(s): Anna Biela; Alexander Hammermeister; Igor Kaczmarczyk; Marta Walczak; Lukasz Koziej; Ting-Yu Lin; Sebastian Glatt
Published in: Journal of biological chemistry, 2023, Page(s) 104966, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2023.104966

Molecular basis for thiocarboxylation and release of Urm1 by its E1-activating enzyme Uba4

Author(s): Mikołaj Sokołowski, Dominika Kwasna, Keerthiraju E Ravichandran, Cristian Eggers, Rościsław Krutyhołowa, Magdalena Kaczmarczyk, Bozena Skupien-Rabian, Marcin Jaciuk, Marta Walczak, Priyanka Dahate, Marta Pabis, Małgorzata Jemioła-Rzemińska, Urszula Jankowska, Sebastian A Leidel, Sebastian Glatt
Published in: Nucleic Acids Research, Issue 52, 2024, Page(s) 13980-13995, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkae1111

Modulation of translational decoding by m6A modification of mRNA

Author(s): Sakshi Jain, Lukasz Koziej, Panagiotis Poulis, Igor Kaczmarczyk, Monika Gaik, Michal Rawski, Namit Ranjan, Sebastian Glatt, Marina V Rodnina
Published in: Nature Communications, 2023, Page(s) 4784, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-40422-7

RNA sample optimization for cryo-EM analysis

Author(s): Xingyu Chen, Liu Wang, Jiahao Xie, Jakub S. Nowak, Bingnan Luo, Chong Zhang, Guowen Jia, Jian Zou, Dingming Huang, Sebastian Glatt, Yang Yang, Zhaoming Su
Published in: Nature Protocols, Issue 20, 2025, Page(s) 1114-1157, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/s41596-024-01072-1

Suboptimal codon pairs trigger ribosome collisions and cellular quality control responses in tRNA modification mutants

Author(s): Jie Wu, Cristian Eggers, Olga Sin, Łukasz Koziej, Hector Mancilla, Fabienne Mollet, Hans R Schöler, Hannes C A Drexler, Tristan Ranff, Christian Fufezan, Claudine Kraft, Sebastian Glatt, Jan M Bruder, Sebastian A Leidel
Published in: Nucleic Acids Research, Issue 53, 2025, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaf1311

E2/E3-independent ubiquitin-like protein conjugation by Urm1 is directly coupled to cysteine persulfidation.

Author(s): Keerthiraju E Ravichandran; Lars Kaduhr; Bozena Skupien‐Rabian; Ekaterina Shvetsova; Mikołaj Sokołowski; Ros´cisław Krutyhołowa; Dominika Kwasna; Cindy Brachmann; Sean Lin; Sebastian Guzman Perez; Piotr Wilk; Manuel Kösters; Przemysław Grudnik; Urszula Jankowska; Sebastian A Leidel; Raffael Schaffrath; Sebastian Glatt
Published in: The EMBO Journal, 2022, ISSN 1460-2075
Publisher: John Wiley & Sons, Ltd
DOI: 10.48350/173059

Structural Snapshots of Human tRNA Modifying Enzymes

Author(s): Alexander Hammermeister, Monika Gaik, Priyanka Dahate, Sebastian Glatt
Published in: Journal of Molecular Biology, Issue 437, 2025, Page(s) 169106, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2025.169106

Ncs2* mediates <i>in vivo</i> virulence of pathogenic yeast through sulphur modification of cytoplasmic transfer RNA

Author(s): Fiona Alings; Karin Scharmann; Cristian Eggers; Bettina Böttcher; Mikołaj Sokołowski; Ekaterina Shvetsova; Puneet Sharma; Joël Roth; Leon Rashiti; Sebastian Glatt; Sascha Brunke; Sebastian A Leidel
Published in: Nucleic Acids Research, Issue 51(15), 2023, Page(s) 8133-8149, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/nar/gkad564

Queuosine is incorporated into precursor tRNA before splicing

Author(s): Wei Guo, Igor Kaczmarczyk, Kevin Kopietz, Florian Flegler, Stefano Russo, Ege Cigirgan, Andrzej Chramiec-Głąbik, Łukasz Koziej, Cansu Cirzi, Jirka Peschek, Klaus Reuter, Mark Helm, Sebastian Glatt, Francesca Tuorto
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-025-62220-z

3-Mercaptopyruvate sulfur transferase is a protein persulfidase

Author(s): Brandán Pedre; Deepti Talwar; Uladzimir Barayeu; Danny Schilling; Marcin Luzarowski; Mikolaj Sokolowski; Sebastian Glatt; Tobias P. Dick
Published in: Nature Chemical Biology, Issue 1, 2023, Page(s) 507–517, ISSN 1552-4469
Publisher: Springer Nature
DOI: 10.1038/s41589-022-01244-8