Deciphering the role of RNA modifications during ribosomal decoding and protein synthesis

Informazioni relative al progetto

tRNAslation

ID dell’accordo di sovvenzione: 101001394

DOI

10.3030/101001394

Data della firma CE 2 Aprile 2021

Data di avvio 1 Giugno 2021

Data di completamento 30 Novembre 2026

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 1 997 500,00

Contributo UE

€ 1 997 500,00

Coordinato da

UNIWERSYTET JAGIELLONSKI
Poland

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (26)

Determining the effects of pseudouridine incorporation on human tRNAs

Autori: Anna D Biela, Jakub S Nowak, Artur P Biela, Sunandan Mukherjee, Seyed Naeim Moafinejad, Satyabrata Maiti, Andrzej Chramiec-Głąbik, Rahul Mehta, Jakub Jeżowski, Dominika Dobosz, Priyanka Dahate, Veronique Arluison, Frank Wien, Paulina Indyka, Michal Rawski, Janusz M Bujnicki, Ting-Yu Lin, Sebastian Glatt
Pubblicato in: The EMBO Journal, Numero 44, 2025, Pagina/e 3553-3585, ISSN 1460-2075
Editore: Springer Science and Business Media LLC
DOI: 10.1038/s44318-025-00443-y

Functional divergence of the two Elongator subcomplexes during neurodevelopment

Autori: Monika Gaik; Marija Kojic; Megan R Stegeman; Tülay Öncü‐Öner; Anna Kościelniak; Alun Jones; Ahmed Mohamed; Pak Yan Stefanie Chau; Sazia Sharmin; Andrzej Chramiec‐Głąbik; Paulina Indyka; Michał Rawski; Anna Biela; Dominika Dobosz; Amanda Millar; Vann Chau; Aycan Ünalp; Michael Piper; Mark C Bellingham; Evan E Eichler; Deborah A Nickerson; Handan Güleryüz; Nour El Hana Abbassi; Konrad
Pubblicato in: EMBO Molecular Medicine, Numero Volume 14, Numero 7, 2022, ISSN 1757-4676
Editore: John Wiley & Sons Ltd.
DOI: 10.15252/emmm.202115608

It takes two to tRNAgo

Autori: Igor Kaczmarczyk; Łukasz Koziej; Sebastian Glatt
Pubblicato in: Structure, Numero Volume 32, Numero 3, 2024, ISSN 0969-2126
Editore: Cell Press
DOI: 10.1016/j.str.2024.02.005

Pseudouridines in RNAs: switching atoms means shifting paradigms.

Autori: Ting-Yu Lin; Rahul Mehta; Sebastian Glatt
Pubblicato in: FEBS Letters, Numero 595, 2021, ISSN 1873-3468
Editore: John Wiley & Sons Ltd
DOI: 10.1002/1873-3468.14188

Structural and biochemical characterization of the 3′-5′ tRNA splicing ligases

Autori: Sebastian Chamera, Weronika Zajko, Mariusz Czarnocki-Cieciura, Marcin Jaciuk, Łukasz Koziej, Jakub Nowak, Krzysztof Wycisk, Małgorzata Sroka, Andrzej Chramiec-Głąbik, Mirosław Śmietański, Filip Gołębiowski, Marcin Warmiński, Jacek Jemielity, Sebastian Glatt, Marcin Nowotny
Pubblicato in: Journal of Biological Chemistry, Numero 301, 2025, Pagina/e 108506, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2025.108506

Autophosphorylation of conserved yeast and human casein kinase 1 isozymes regulates Elongator-dependent tRNA modifications

Autori: Maria Friederike Landrock, Rościsław Krutyhołowa, Pauline Böhnert, Jarosław Mazur, Małgorzata Honc, Alexander Hammermeister, Larissa Bessler, David Scherf, Anna Elms, Natalia Radczuk, Bozena Skupien-Rabian, Urszula Jankowska, Friedrich W Herberg, Mark Helm, Roland Klassen, Sebastian Glatt, Raffael Schaffrath
Pubblicato in: Nucleic Acids Research, Numero 53, 2025, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkaf881

Mapping rRNA, tRNA, and mRNA modifications in ribosomes at high resolution

Autori: Łukasz Koziej, Sebastian Glatt
Pubblicato in: Trends in Biochemical Sciences, Numero 50, 2026, Pagina/e 1027-1041, ISSN 0968-0004
Editore: Elsevier BV
DOI: 10.1016/j.tibs.2025.08.007

tRNA binding to Kti12 is crucial for wobble uridine modification by Elongator

Autori: David Scherf, Alexander Hammermeister, Pauline Böhnert, Alicia Burkard, Mark Helm, Sebastian Glatt, Raffael Schaffrath
Pubblicato in: Nucleic Acids Research, Numero 53, 2025, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkaf296

Cryo-EM structure of the fully assembled Elongator complex

Autori: Marcin Jaciuk; David Scherf; Karol Kaszuba; Monika Gaik; Alexander Rau; Anna Kościelniak; Rościsław Krutyhołowa; Michał Rawski; Paulina Indyka; Andrea Graziadei; Andrzej Chramiec-Głąbik; Anna Biela; Dominika Dobosz; Ting-Yu Lin; Nour-el-Hana Abbassi; Alexander Hammermeister; Juri Rappsilber; Jan Kosinski; Raffael Schaffrath; Sebastian Glatt
Pubblicato in: Nucleic Acids Research, 2023, ISSN 1362-4962
Editore: Oxford University Press
DOI: 10.1093/nar/gkac1232

Conserved structures and dynamics in 5′-proximal regions of Betacoronavirus RNA genomes

Autori: Tales Rocha de Moura, Elżbieta Purta, Agata Bernat, Eva M Martín-Cuevas, Małgorzata Kurkowska, Eugene F Baulin, Sunandan Mukherjee, Jakub Nowak, Artur P Biela, Michał Rawski, Sebastian Glatt, Fernando Moreno-Herrero, Janusz M Bujnicki
Pubblicato in: Nucleic Acids Research, Numero 52, 2024, Pagina/e 3419-3432, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkae144

The molecular basis of tRNA selectivity by human pseudouridine synthase 3

Autori: Ting-Yu Lin, Leon Kleemann, Jakub Jeżowski, Dominika Dobosz, Michał Rawski, Paulina Indyka, Grzegorz Ważny, Rahul Mehta, Andrzej Chramiec-Głąbik, Łukasz Koziej, Tristan Ranff, Christian Fufezan, Mateusz Wawro, Jakub Kochan, Joanna Bereta, Sebastian A. Leidel, Sebastian Glatt
Pubblicato in: Molecular Cell, Numero 84, 2024, Pagina/e 2472-2489.e8, ISSN 1097-2765
Editore: Cell Press
DOI: 10.1016/j.molcel.2024.06.013

Mechanistic insight into the pseudouridylation of RNA

Autori: Ting-Yu Lin, Yasmin Stone, Sebastian Glatt
Pubblicato in: RNA Biology, Numero 22, 2025, Pagina/e 1-25, ISSN 1547-6286
Editore: Landes Bioscience
DOI: 10.1080/15476286.2025.2541421

Cryo-EM structures of the human Elongator complex at work

Autori: Nour-el-Hana Abbassi, Marcin Jaciuk, David Scherf, Pauline Böhnert, Alexander Rau, Alexander Hammermeister, Michał Rawski, Paulina Indyka, Grzegorz Wazny, Andrzej Chramiec-Głąbik, Dominika Dobosz, Bozena Skupien-Rabian, Urszula Jankowska, Juri Rappsilber, Raffael Schaffrath, Ting-Yu Lin, Sebastian Glatt
Pubblicato in: Nature Communications, Numero 15, 2024, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-024-48251-y

Elongator and the role of its subcomplexes in human diseases

Autori: Monika Gaik; Marija Kojic; Brandon J Wainwright; Sebastian Glatt
Pubblicato in: EMBO Molecular Medicine, Numero 1, 2022, Pagina/e 1757-4676, ISSN 1757-4676
Editore: John Wiley & Sons Ltd.
DOI: 10.15252/emmm.202216418

Destabilization of mutated human PUS3 protein causes intellectual disability

Autori: Ting-Yu Lin, Robert Smigiel, Bozena Kuzniewska, Joanna J. Chmielewska, Joanna Kosińska, Mateusz Biela, Anna Biela, Anna Kościelniak, Dominika Dobosz, Izabela Laczmanska, Andrzej Chramiec-Głąbik, Jakub Jeżowski, Jakub Nowak, Monika Gos, Sylwia Rzonca-Niewczas, Magdalena Dziembowska, Rafał Ploski, Sebastian Glatt
Pubblicato in: Human Mutation, 2022, Pagina/e 2063-2078, ISSN 1098-1004
Editore: John Wiley & Sons
DOI: 10.1002/humu.24471

A novel ELP1 mutation impairs the function of the Elongator complex and causes a severe neurodevelopmental phenotype

Autori: Marija Kojic, Nour E. H. Abbassi, Ting-Yu Lin, Alun Jones, Emma L. Wakeling, Emma Clement, Vasiliki Nakou, Matthew Singleton, Dominika Dobosz, Marios Kaliakatsos, Sebastian Glatt & Brandon J. Wainwright
Pubblicato in: J Hum Genet, 2023, Pagina/e 445-453, ISSN 1434-5161
Editore: Springer Verlag
DOI: 10.1038/s10038-023-01135-3

The diverse structural modes of tRNA binding and recognition

Autori: Anna Biela; Alexander Hammermeister; Igor Kaczmarczyk; Marta Walczak; Lukasz Koziej; Ting-Yu Lin; Sebastian Glatt
Pubblicato in: Journal of biological chemistry, 2023, Pagina/e 104966, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2023.104966

Molecular basis for thiocarboxylation and release of Urm1 by its E1-activating enzyme Uba4

Autori: Mikołaj Sokołowski, Dominika Kwasna, Keerthiraju E Ravichandran, Cristian Eggers, Rościsław Krutyhołowa, Magdalena Kaczmarczyk, Bozena Skupien-Rabian, Marcin Jaciuk, Marta Walczak, Priyanka Dahate, Marta Pabis, Małgorzata Jemioła-Rzemińska, Urszula Jankowska, Sebastian A Leidel, Sebastian Glatt
Pubblicato in: Nucleic Acids Research, Numero 52, 2024, Pagina/e 13980-13995, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkae1111

Modulation of translational decoding by m6A modification of mRNA

Autori: Sakshi Jain, Lukasz Koziej, Panagiotis Poulis, Igor Kaczmarczyk, Monika Gaik, Michal Rawski, Namit Ranjan, Sebastian Glatt, Marina V Rodnina
Pubblicato in: Nature Communications, 2023, Pagina/e 4784, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-40422-7

RNA sample optimization for cryo-EM analysis

Autori: Xingyu Chen, Liu Wang, Jiahao Xie, Jakub S. Nowak, Bingnan Luo, Chong Zhang, Guowen Jia, Jian Zou, Dingming Huang, Sebastian Glatt, Yang Yang, Zhaoming Su
Pubblicato in: Nature Protocols, Numero 20, 2025, Pagina/e 1114-1157, ISSN 1754-2189
Editore: Nature Publishing Group
DOI: 10.1038/s41596-024-01072-1

Suboptimal codon pairs trigger ribosome collisions and cellular quality control responses in tRNA modification mutants

Autori: Jie Wu, Cristian Eggers, Olga Sin, Łukasz Koziej, Hector Mancilla, Fabienne Mollet, Hans R Schöler, Hannes C A Drexler, Tristan Ranff, Christian Fufezan, Claudine Kraft, Sebastian Glatt, Jan M Bruder, Sebastian A Leidel
Pubblicato in: Nucleic Acids Research, Numero 53, 2025, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkaf1311

E2/E3-independent ubiquitin-like protein conjugation by Urm1 is directly coupled to cysteine persulfidation.

Autori: Keerthiraju E Ravichandran; Lars Kaduhr; Bozena Skupien‐Rabian; Ekaterina Shvetsova; Mikołaj Sokołowski; Ros´cisław Krutyhołowa; Dominika Kwasna; Cindy Brachmann; Sean Lin; Sebastian Guzman Perez; Piotr Wilk; Manuel Kösters; Przemysław Grudnik; Urszula Jankowska; Sebastian A Leidel; Raffael Schaffrath; Sebastian Glatt
Pubblicato in: The EMBO Journal, 2022, ISSN 1460-2075
Editore: John Wiley & Sons, Ltd
DOI: 10.48350/173059

Structural Snapshots of Human tRNA Modifying Enzymes

Autori: Alexander Hammermeister, Monika Gaik, Priyanka Dahate, Sebastian Glatt
Pubblicato in: Journal of Molecular Biology, Numero 437, 2025, Pagina/e 169106, ISSN 0022-2836
Editore: Academic Press
DOI: 10.1016/j.jmb.2025.169106

Ncs2* mediates <i>in vivo</i> virulence of pathogenic yeast through sulphur modification of cytoplasmic transfer RNA

Autori: Fiona Alings; Karin Scharmann; Cristian Eggers; Bettina Böttcher; Mikołaj Sokołowski; Ekaterina Shvetsova; Puneet Sharma; Joël Roth; Leon Rashiti; Sebastian Glatt; Sascha Brunke; Sebastian A Leidel
Pubblicato in: Nucleic Acids Research, Numero 51(15), 2023, Pagina/e 8133-8149, ISSN 1362-4962
Editore: Oxford University Press
DOI: 10.1093/nar/gkad564

Queuosine is incorporated into precursor tRNA before splicing

Autori: Wei Guo, Igor Kaczmarczyk, Kevin Kopietz, Florian Flegler, Stefano Russo, Ege Cigirgan, Andrzej Chramiec-Głąbik, Łukasz Koziej, Cansu Cirzi, Jirka Peschek, Klaus Reuter, Mark Helm, Sebastian Glatt, Francesca Tuorto
Pubblicato in: Nature Communications, Numero 16, 2025, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-025-62220-z

3-Mercaptopyruvate sulfur transferase is a protein persulfidase

Autori: Brandán Pedre; Deepti Talwar; Uladzimir Barayeu; Danny Schilling; Marcin Luzarowski; Mikolaj Sokolowski; Sebastian Glatt; Tobias P. Dick
Pubblicato in: Nature Chemical Biology, Numero 1, 2023, Pagina/e 507–517, ISSN 1552-4469
Editore: Springer Nature
DOI: 10.1038/s41589-022-01244-8