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Nanobodies and antibodies against 2019-nCoV

Deliverables

CoroNAb website for dissemination of results, and for fostering broader protocol, reagent, and result sharing by the scientific community

​CoroNAb website for dissemination of results, and for fostering broader protocol, reagent, and result sharing by the scientific community.

Formulation characteristics and physicochemical evaluation of antigen-adjuvant combinations

Formulation characteristics and physicochemical evaluation of antigen-adjuvant combinations.

Shared plasmid design

We will share out plasmid designs, right from the start of the project. Initially, this will be through our collaboration network, and subsequently this will be on the CoroNAb website.

Shared 2019-nCoV spike binding results for mAbs

Shared 2019-nCoV spike binding results for mAbs.

Shared nanobody sequences of confirmed binders, and data from NGS library sequencing

Shared nanobody sequences of confirmed binders, and data from NGS library sequencing.

Shared 2019-nCoV spike binding results for nanobodies

Shared 2019-nCoV spike binding results for nanobodies.

Shared mAb sequences, including NGS data

D3.1​ Shared mAb sequences, including NGS data.

Safety and immunogenicity data for each subunit /adjuvant combination. Selection of the best nCoV spike protein and adjuvant combination for immunizations of larger animals

Safety and immunogenicity data for each subunit/adjuvant combination. Selection of the best nCoV spike protein and adjuvant combination for immunizations of larger animals.

Update of DMP

The draft management plan will be updated.

Neutralization results for isolated monoclonal antibodies and camelid nanobodies, as well as modified mAbs

D4.2.​ Neutralization results for isolated monoclonal antibodies and camelid nanobodies (aims 2

Mathematical model will be released as open source R package. Model projections will be published online.

Mathematical model will be released as open source R package. Model projections will be published online.

Shared expression results

Shared expression results, yields, and working protocols for all constructs. This will be ongoing throughout the project.

Publications

Assessing transmissibility of SARS-CoV-2 lineage B.1.1.7 in England

Author(s): Erik Volz, Swapnil Mishra, Meera Chand, Jeffrey C. Barrett, Robert Johnson, Lily Geidelberg, Wes R. Hinsley, Daniel J. Laydon, Gavin Dabrera, Áine O’Toole, Robert Amato, Manon Ragonnet-Cronin, Ian Harrison, Ben Jackson, Cristina V. Ariani, Olivia Boyd, Nicholas J. Loman, John T. McCrone, Sónia Gonçalves, David Jorgensen, Richard Myers, Verity Hill, David K. Jackson, Katy Gaythorpe, Natalie Gr
Published in: Nature, Issue 593/7858, 2021, Page(s) 266-269, ISSN 0028-0836
DOI: 10.1038/s41586-021-03470-x

DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immune responses in mice

Author(s): Inga Szurgot, Leo Hanke, Daniel J. Sheward, Laura Perez Vidakovics, Ben Murrell, Gerald M. McInerney, Peter Liljeström
Published in: Scientific Reports, Issue 11/1, 2021, ISSN 2045-2322
DOI: 10.1038/s41598-021-82498-5

SARS-CoV-2 protein subunit vaccination of mice and rhesus macaques elicits potent and durable neutralizing antibody responses

Author(s): Marco Mandolesi, Daniel J. Sheward, Leo Hanke, Junjie Ma, Pradeepa Pushparaj, Laura Perez Vidakovics, Changil Kim, Monika Àdori, Klara Lenart, Karin Loré, Xaquin Castro Dopico, Jonathan M. Coquet, Gerald M. McInerney, Gunilla B. Karlsson Hedestam, Ben Murrell
Published in: Cell Reports Medicine, Issue 2/4, 2021, Page(s) 100252, ISSN 2666-3791
DOI: 10.1016/j.xcrm.2021.100252

Genetic evidence for the association between COVID-19 epidemic severity and timing of non-pharmaceutical interventions

Author(s): Manon Ragonnet-Cronin, Olivia Boyd, Lily Geidelberg, David Jorgensen, Fabricia F. Nascimento, Igor Siveroni, Robert A. Johnson, Marc Baguelin, Zulma M. Cucunubá, Elita Jauneikaite, Swapnil Mishra, Oliver J. Watson, Neil Ferguson, Anne Cori, Christl A. Donnelly, Erik Volz
Published in: Nature Communications, Issue 12/1, 2021, ISSN 2041-1723
DOI: 10.1038/s41467-021-22366-y

Picomolar SARS-CoV-2 Neutralization Using Multi-Arm PEG Nanobody Constructs

Author(s): Ainhoa Moliner-Morro, Daniel J. Sheward, Vivien Karl, Laura Perez Vidakovics, Ben Murrell, Gerald M. McInerney, Leo Hanke
Published in: Biomolecules, Issue 10/12, 2020, Page(s) 1661, ISSN 2218-273X
DOI: 10.3390/biom10121661

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2

Author(s): Tânia F. Custódio, Hrishikesh Das, Daniel J. Sheward, Leo Hanke, Samuel Pazicky, Joanna Pieprzyk, Michèle Sorgenfrei, Martin A. Schroer, Andrey Yu. Gruzinov, Cy M. Jeffries, Melissa A. Graewert, Dmitri I. Svergun, Nikolay Dobrev, Kim Remans, Markus A. Seeger, Gerald M. McInerney, Ben Murrell, B. Martin Hällberg, Christian Löw
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
DOI: 10.1038/s41467-020-19204-y

Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape

Author(s): Paul-Albert Koenig, Hrishikesh Das, Hejun Liu, Beate M. Kümmerer, Florian N. Gohr, Lea-Marie Jenster, Lisa D. J. Schiffelers, Yonas M. Tesfamariam, Miki Uchima, Jennifer D. Wuerth, Karl Gatterdam, Natalia Ruetalo, Maria H. Christensen, Caroline I. Fandrey, Sabine Normann, Jan M. P. Tödtmann, Steffen Pritzl, Leo Hanke, Jannik Boos, Meng Yuan, Xueyong Zhu, Jonathan L. Schmid-Burgk, Hiroki Kato, Mi
Published in: Science, Issue 371/6530, 2021, Page(s) eabe6230, ISSN 0036-8075
DOI: 10.1126/science.abe6230

Seropositivity in blood donors and pregnant women during the first year of SARS‐CoV‐2 transmission in Stockholm, Sweden

Author(s): X. Castro Dopico, S. Muschiol, M. Christian, L. Hanke, D. J. Sheward, N. F. Grinberg, J. Rorbach, G. Bogdanovic, G. M. Mcinerney, T. Allander, C. Wallace, B. Murrell, J. Albert, G. B. Karlsson Hedestam
Published in: Journal of Internal Medicine, 2021, ISSN 0954-6820
DOI: 10.1111/joim.13304

An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction

Author(s): Leo Hanke, Laura Vidakovics Perez, Daniel J. Sheward, Hrishikesh Das, Tim Schulte, Ainhoa Moliner-Morro, Martin Corcoran, Adnane Achour, Gunilla B. Karlsson Hedestam, B. Martin Hällberg, Ben Murrell, Gerald M. McInerney
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
DOI: 10.1038/s41467-020-18174-5

Adjuvanted SARS-CoV-2 spike protein elicits neutralizing antibodies and CD4 T cell responses after a single immunization in mice

Author(s): Katharina Wørzner, Daniel J. Sheward, Signe Tandrup Schmidt, Leo Hanke, Julie Zimmermann, Gerald McInerney, Gunilla B. Karlsson Hedestam, Ben Murrell, Dennis Christensen, Gabriel Kristian Pedersen
Published in: EBioMedicine, Issue 63, 2021, Page(s) 103197, ISSN 2352-3964
DOI: 10.1016/j.ebiom.2020.103197

SARS-CoV-2 exposure, symptoms and seroprevalence in healthcare workers in Sweden

Author(s): Ann-Sofie Rudberg, Sebastian Havervall, Anna Månberg, August Jernbom Falk, Katherina Aguilera, Henry Ng, Lena Gabrielsson, Ann-Christin Salomonsson, Leo Hanke, Ben Murrell, Gerald McInerney, Jennie Olofsson, Eni Andersson, Cecilia Hellström, Shaghayegh Bayati, Sofia Bergström, Elisa Pin, Ronald Sjöberg, Hanna Tegel, My Hedhammar, Mia Phillipson, Peter Nilsson, Sophia Hober, Charlotte Thålin
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
DOI: 10.1038/s41467-020-18848-0

Evaluating the Effects of SARS-CoV-2 Spike Mutation D614G on Transmissibility and Pathogenicity

Author(s): Erik Volz, Verity Hill, John T. McCrone, Anna Price, David Jorgensen, Áine O’Toole, Joel Southgate, Robert Johnson, Ben Jackson, Fabricia F. Nascimento, Sara M. Rey, Samuel M. Nicholls, Rachel M. Colquhoun, Ana da Silva Filipe, James Shepherd, David J. Pascall, Rajiv Shah, Natasha Jesudason, Kathy Li, Ruth Jarrett, Nicole Pacchiarini, Matthew Bull, Lily Geidelberg, Igor Siveroni, Ian Goodfellow
Published in: Cell, Issue 184/1, 2021, Page(s) 64-75.e11, ISSN 0092-8674
DOI: 10.1016/j.cell.2020.11.020