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Coherent Raman Imaging for the Molecular Study of the OrigiN of diseases

Project description

Molecular analysis of disease through a novel microscopy approach

The complexity and heterogeneity of diseases such as cancer require extensive research to determine the cellular and biochemical origin of the disease. To assist these efforts, scientists of the EU-funded CRIMSON project propose to develop a novel coherent Raman scattering (CRS)-based methodology that relies on the vibration of molecules. Combined with AI, this method will enable imaging in ex vivo thick slides and ultimately inside the body through a CRS endoscope. The CRIMSON system will facilitate cell and tissue classification with unprecedented biochemical sensitivity, paving the way for the design of personalised treatments.

Objective

CRIMSON aims to provide a next-generation bio-photonics imaging device based on vibrational spectroscopy, with the potential to revolutionise the study of the cellular origin of diseases allowing for novel approaches towards personalised therapy. We will employ label-free broadband coherent Raman scattering (CRS) extended to the fingerprint region, in combination with artificial-intelligence spectroscopic data analysis, for fast cell/tissue classification with unprecedented biochemical sensitivity. We will develop a hyperspectral CRS microscope for 3D quantitative imaging of sub-cellular compartments in living cells and organoids. High acquisition speed will enable the observation of intra- and inter-cellular dynamic changes by time-lapse imaging. We will simulate future in-vivo studies and demonstrate the capability to image inside the body, realizing an innovative CRS endoscope and applying it to ex-vivo thick tissue slides. CRIMSON relies on the development of new compact ultrafast lasers, innovative broadband CRS detection schemes and advanced spectral analysis routines.
To validate the CRS platform, we will investigate three open biological questions related to cancer, as paradigmatic examples of the complexity and heterogeneity of cellular diseases. The results will have profound societal impacts, improving patients’ quality of life and reducing public healthcare costs.
CRIMSON brings together a multidisciplinary team of world-leading academic organizations, biomedical end users and innovative SMEs, with vertical integration of all required skills. CRIMSON will bridge the gap between research and product development, increasing the TRL and making CRS a user-friendly, robust and cost-effective mainstream tool for a vast biological research community. Commercial exploitation by the participating SMEs, including a biomedical equipment manufacturer, will create a competitive advantage in the European biophotonics-related market for microscopes and R&D tools.

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Topic(s)

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Funding Scheme

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RIA - Research and Innovation action

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Call for proposal

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(opens in new window) H2020-ICT-2018-20

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Coordinator

POLITECNICO DI MILANO
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 880 787,50
Address
PIAZZA LEONARDO DA VINCI 32
20133 Milano
Italy

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Region
Nord-Ovest Lombardia Milano
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 016 287,50

Participants (11)

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