Coherent Raman Imaging for the Molecular Study of the OrigiN of diseases

Project Information

CRIMSON

Grant agreement ID: 101016923

DOI 10.3030/101016923

Project closed

EC signature date 30 November 2020

Start date 1 December 2020

End date 31 May 2024

Funded under

• INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies - Information and Communication Technologies (ICT)

Total cost € 5 132 801,25

EU contribution € 5 132 801,25

Coordinated by

POLITECNICO DI MILANO
Italy

Deliverables

Open Research Data Pilot (1)

D6.1 Data Management Plan

Data Management Plan

Websites, patent fillings, videos etc. (3)

D5.4 Preliminary communication kit

Preliminary communication kit that will be updated throughout the project

D5.1 Project website, graphical identity of the CRIMSON project, project flyer and database of stakeholders

Project website graphical identity of the CRIMSON project project flyer and database of stakeholders

D5.5 Communication kit (mid-term)

Communication kit updated at M18

Publications

Peer reviewed articles (31)

Comparing Transmission- and Epi-BCARS: A Transnational Round Robin on Solid State Materials

Author(s): Hempel, Franz; Vernuccio, Federico; König, Lukas; Buschbeck, Robin; Rüsing, Michael; Cerullo, Giulio; Polli, Dario; Eng, Lukas M.
Published in: Applied Optics, Issue Vol. 63, Issue 1, 2024, Page(s) 112-121, ISSN 1559-128X
Publisher: Optica
DOI: 10.48550/arxiv.2306.09701

Feasibility studies of multimodal nonlinear endoscopy using multicore fiber bundles for remote scanning from tissue sections to bulk organs

Author(s): Hyeonsoo Bae, Marko Rodewald, Tobias Meyer-Zedler, Thomas W. Bocklitz, Gregor Matz, Bernhard Messerschmidt, Adrian T. Press, Michael Bauer, Orlando Guntinas-Lichius, Andreas Stallmach, Michael Schmitt & Juergen Popp
Published in: Scientific Reports volume 13, Article number: 13779, Issue Scientific Reports volume 13, Article number: 13779, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-40944-6

Deep Ensemble Learning and Transfer Learning Methods for Classification of Senescent Cells from Nonlinear Optical Microscopy Images

Author(s): Salvatore Sorrentino, Francesco Manetti, Arianna Bresci, Federico Vernuccio, Chiara Ceconello, Silvia Ghislanzoni, Italia Bongarzone, Renzo Vanna, Giulio Cerullo, Dario Polli
Published in: Front. Chem., Issue 11, 1213981, 2023, ISSN 2296-2646
Publisher: Frontiers
DOI: 10.3389/fchem.2023.1213981

Noninvasive morpho-molecular imaging reveals early therapy-induced senescence in human cancer cells

Author(s): Arianna Bresci, Jeong Hee Kim, Silvia Ghislanzoni, Francesco Manetti, Lintong Wu, Federico Vernuccio, Chiara Ceconello, Salvatore Sorrentino, Ishan Barman, Italia Bongarzone, Giulio Cerullo, Renzo Vanna, And Dario Polli
Published in: Science Advances, Issue 9, eadg6231, 2023, ISSN 2375-2548
Publisher: American Association for the Advancement of Science
DOI: 10.1126/sciadv.adg6231

Reduced sulfatide content in deferoxamine-induced senescent HepG2 cells

Author(s): Silvia Ghislanzoni, Gaia Martina Sarcinelli, Arianna Bresci, Francesco Manetti, Dario Polli, Antonella Tomassetti, Maria Teresa Radice, Italia Bongarzone
Published in: Int J Biochem Cell Biol ., Issue Vol. 159, 2023, ISSN 1357-2725
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.biocel.2023.106419

Full-Spectrum CARS Microscopy of Cells and Tissues with Ultrashort White-Light Continuum Pulses

Author(s): Federico Vernuccio, Renzo Vanna, Chiara Ceconello, Arianna Bresci, Francesco Manetti, Salvatore Sorrentino, Silvia Ghislanzoni, Flavia ambertucci, Omar Motiño, Isabelle Martins, Guido Kroemer, Italia Bongarzone, Giulio Cerullo, and Dario Polli
Published in: J. Phys. Chem., Issue B 127, 21, 2023, Page(s) 4733–4745, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcb.3c01443

Experimental determination of shift-less aberration bases for sensorless adaptive optics in nonlinear microscopy

Author(s): Benedetta Talone; Paolo Pozzi; Miriam Cavagnini; Dario Polli; Gianluca Pozzi; Jonathan Mapelli
Published in: Optics Express, Issue 29, 2021, Page(s) 37617-37627, ISSN 2334-2536
Publisher: Optica
DOI: 10.1364/oe.435262

Selective Detection in Impulsive Low-Frequency Raman Imaging Using Shaped Probe Pulses

Author(s): Siddarth Shivkumar, Dekel Ranann, Samuel Metais, Sisira Suresh, Nicolas Forget, Randy Bartels, Dan Oron, and Hervé Rigneault
Published in: Phys. Rev. Applied, Issue 19, 054075, 2023, ISSN 2643-1564
Publisher: American Physical Sociery
DOI: 10.1103/physrevapplied.19.054075

Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions

Author(s): Benedetta Talone, Arianna Bresci, Francesco Manetti, Federico Vernuccio, Alejandro De La Cadena, Chiara Ceconello, Maria L. Schiavone, Stefano Mantero, Ciro Menale, Renzo Vanna, Giulio Cerullo, Cristina Sobacchi and Dario Polli
Published in: Front. Bioeng. Biotechnol., Issue 22, 10:1042680, 2022, ISSN 2296-4185
Publisher: Frontiers
DOI: 10.3389/fbioe.2022.1042680

Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation

Author(s): Chenting Lai, Matteo Calvarese, Karl Reichwald, Hyeonsoo Bae, Mohammadsadegh Vafaeinezhad, Tobias Meyer-Zedler, Franziska Hoffmann, Anna Mühlig, Tino Eidam, Fabian Stutzki, Bernhard Messerschmidt, Herbert Gross, Michael Schmitt, Orlando Guntinas-Lichius, Jürgen Popp
Published in: Journal of Biomedical Optics, Issue Vol. 28, Issue 6, 2023, ISSN 1560-2281
Publisher: SPIE
DOI: 10.1117/1.jbo.28.6.066004

An obesogenic feedforward loop involving PPARγ, acyl-CoA binding protein and GABA receptor

Author(s): Gerasimos Anagnostopoulos, Omar Motiño, Sijing Li, Vincent Carbonnier, Hui Chen, Valentina Sica, Sylvère Durand, Mélanie Bourgin, Fanny Aprahamian, Nitharsshini Nirmalathasan, Romain Donne, Chantal Desdouets, Marcelo Simon Sola, Konstantina Kotta, Léa Montégut, Flavia Lambertucci, Didier Surdez, Grossetête Sandrine, Olivier Delattre, Maria Chiara Maiuri, José Manuel Bravo-San Pedro, Isabell
Published in: Cell Death & Desease, Issue 13(4), 2022, ISSN 2041-4889
Publisher: Nature Publishing Group
DOI: 10.1038/s41419-022-04834-5

Nearly degenerate two-color impulsive coherent Raman hyperspectral imaging

Author(s): David R. Smith, Siddarth Shivkumar, Jeff Field, Jesse W. Wilson, Hervé Rigneault, and Randy A. Bartels
Published in: Optics Letters, Issue Vol. 47, Issue 22,, 2022, Page(s) 5841-5844, ISSN 2334-2536
Publisher: Optica
DOI: 10.1364/ol.467970

Deep reinforcement learning control of white-light continuum generation

Author(s): Carlo Michele Valensise; Alessandro Giuseppi; Giulio Cerullo; Dario Polli
Published in: Optica, Issue 8, 2021, Page(s) 239-242, ISSN 2334-2536
Publisher: Optica
DOI: 10.1364/optica.414634

Ultrafast Spectral Tuning of a Fiber Laser for Time-Encoded Multiplex Coherent Raman Scattering Microscopy

Author(s): Thomas Gottschall, Tobias Meyer-Zedler, Matthias Eibl, Tom Pfeiffer, Hubertus Hakert, Michael Schmitt, Robert Huber, Andreas Tünnermann, Jens Limpert, Juergen Popp
Published in:  J. Phys. Chem. B, Issue 127, 11, 2023, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcb.2c09115

Double-modulations stimulated Raman scattering: how to image up to 16-fold faster

Author(s): Sandro Heuke, Xavier Audier, Hervé Rigneault
Published in: Optics Letters, Issue 15; 48(2), 2023, Page(s) 423-426, ISSN 2334-2536
Publisher: Optica
DOI: 10.1364/ol.467514

Photon-noise: Is a single-pixel camera better than point scanning? A signal-to-noise ratio analysis for Hadamard and Cosine positive modulation

Author(s): Camille Scotté, Frédéric Galland and Hervé Rigneault
Published in: J. Phys. Photonics, Issue 5, 2023, ISSN 1742-6596
Publisher: IOP Publishing
DOI: 10.48550/arxiv.2210.01915

Optical Diffraction Tomography and Raman Confocal Microscopy for the Investigation of Vacuoles Associated with Cancer Senescent Engulfing Cells

Author(s): Silvia Ghislanzoni; Jeon Woong Kang; Arianna Bresci; Andrea Masella; Koseki J. Kobayashi-Kirschvink; Dario Polli; Italia Bongarzone; Peter T. C. So
Published in: Biosensors, Issue Vol 13, Iss 11, 2023, Page(s) 1-15, ISSN 2079-6374
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.20944/preprints202310.0573.v1

Broadband Four-Wave Mixing Enhanced by Plasmonic Surface Lattice Resonance and Localized Surface Plasmon Resonance in an Azimuthally Chirped Grating

Author(s): Abhik Chakraborty, Parijat Barman, Ankit Kumar Singh, Xiaofei Wu, Denis A. Akimov, Tobias Meyer-Zedler, Stefan Nolte, Carsten Ronning, Michael Schmitt, Jürgen Popp, Jer-Shing Huang
Published in: Laser & Photonics Reviews, Issue Volume 17, Issue 7, 2023, ISSN 1863-8899
Publisher: WILEY Online LIBRARY
DOI: 10.1002/lpor.202200958

Broadband stimulated Raman imaging based on multi-channel lock-in detection for spectral histopathology

Author(s): Alejandro De la Cadena, Federico Vernuccio, Andrea Ragni, Giuseppe Sciortino, Renzo Vanna, Carino Ferrante, Natalia Pediconi, Carlo Valensise, Luca Genchi, Sergey P. Laptenok, Andrea Doni, Marco Erreni, Tullio Scopigno, Carlo Liberale, Giorgio Ferrari, Marco Sampietro, Giulio Cerullo, Dario Polli
Published in: APL Photonics, Issue 7, 2022, ISSN 2378-0967
Publisher: APL Photonics
DOI: 10.1063/5.0093946

Removal of cross-phase modulation artifacts in ultrafast pump–probe dynamics by deep learning

Author(s): A. Bresci; Michele Guizzardi; Carlo Michele Valensise; Fabio Marangi; Francesco Scotognella; Giulio Cerullo; Dario Polli
Published in: APL Photonics, Issue 6, 2021, Page(s) 076104, ISSN 2378-0967
Publisher: American Institute of Physics (United States)
DOI: 10.1063/5.0057404

Nonlinear Optical Signal Generation Mediated by a Plasmonic Azimuthally Chirped Grating

Author(s): Parijat Barman, Abhik Chakraborty, Denis A. Akimov, Ankit Kumar Singh, Tobias Meyer-Zedler, Xiaofei Wu, Carsten Ronning, Michael Schmitt, Jürgen Popp*, and Jer-Shing Huan
Published in: Nano Lett. 2022, 22, 24, 9914–9919, Issue Nano Lett. 2022, 22, 24, 9914–9919, 2022, Page(s) 9914–9919, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.2c03348

Live Stimulated Raman Histology for the Near-Instant Assessment of Central Nervous System Samples

Author(s): Romain Appay, Barbara Sarri, Sandro Heuke, Sébastien Boissonneau, Chang Liu, Etienne Dougy, Laurent Daniel, Didier Scavarda, Henry Dufour, Dominique Figarella-Branger, and Hervé Rigneault
Published in: Physical Chemistry B, Issue 127, 16, 2023, Page(s) 3624–3631, ISSN 1463-9076
Publisher: Royal Society of Chemistry
DOI: 10.1021/acs.jpcb.3c01156

High-throughput label-free detection of DNA-to-RNA transcription inhibition using brightfield microscopy and deep neural networks

Author(s): Allan Sauvata; Giulia Cerrato; Juliette Humeau; Marion Leduc; Oliver Kepp; Guido Kroemer
Published in: Computers in biology andmedicine, Issue 133, 2021, ISSN 0010-4825
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.compbiomed.2021.104371

Comparison of denoising tools for the reconstruction of nonlinear multimodal images. Biomedical Optics Express

Author(s): Rola Houhou, Elsie Quansah, Tobias Meyer-Zedler, Michael Schmitt, Franziska Hoffmann, Orlando Guntinas-Lichius, Jürgen Popp, and Thomas Bocklitz
Published in: Biomedical Optics Express, Issue volume 14 issue 7, 2023, Page(s) 3259-3278, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.477384

Phototoxicity induced in living HeLa cells by focused femtosecond laser pulses: a data-driven approach

Author(s): Benedetta Talone; M. Bazzarelli; Andrea Schirato; F. Dello Vicario; Daniele Viola; Emanuela Jacchetti; M. Bregonzio; Manuela Teresa Raimondi; Giulio Cerullo; Dario Polli
Published in: Biomed Opt Express, Issue 12, 2021, Page(s) 7886-7905, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.441225

Fingerprint multiplex CARS at high speed based on supercontinuum generation in bulk media and deep learning spectral denoising

Author(s): Federico Vernuccio, Arianna Bresci, Benedetta Talone, Alejandro De la Cadena, Chiara Ceconello, Stefano Mantero, Cristina Sobacchi, Renzo Vanna, Giulio Cerullo, and Dario Polli
Published in: Optics Express, Issue 30, 2022, ISSN 1094-4087
Publisher: Optical Society of America
DOI: 10.1364/oe.463032

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Author(s): Alejandro, De la Cadena; Federico, Vernuccio; Benedetta, Talone; Arianna, Bresci; Chiara, Ceconello; Subir, Das; Renzo, Vanna; Giulio, Cerullo; Dario, Polli
Published in: Journal of Visualized Experiments, Issue 185: e63709, 2022, Page(s) 1-25, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/63709

Multi-Class Cancer Subtyping in Salivary Gland Carcinomas with MALDI Imaging and Deep Learning

Author(s): David Pertzborn; Christoph Arolt; Günther Ernst; Oliver J. Lechtenfeld; Jan Kaesler; Daniela Pelzel; Orlando Guntinas-Lichius; Ferdinand von Eggeling; Franziska Hoffmann
Published in: Cancers, Issue 14(17), 2022, ISSN 2072-6694
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers14174342

A Proposal to Perform High Contrast Imaging of Human Palatine Tonsil with Cross Polarized Optical Coherence Tomography

Author(s): Gargi Sharma, Asha Parmar, Franziska Hoffmann, Katharina Geißler, Ferdinand von Eggeling, Orlando Guntinas-Lichius, Kanwarpal Singh
Published in: Photonics, 2022, ISSN 2304-6732
Publisher: MDPI
DOI: 10.3990/photonics9040259

Artificial Intelligence in Classical and Quantum Photonics

Author(s): Federico Vernuccio; Arianna Bresci; Valeria Cimini; Alessandro Giuseppi; Giulio Cerullo; Dario Polli; Carlo Michele Valensise
Published in: Laser & Photonics Reviews, Issue 16, 2022, Page(s) 2100399, ISSN 1863-8899
Publisher: Wiley-VCH
DOI: 10.1002/lpor.202100399

CARS Imaging Advances Early Diagnosis of Cardiac Manifestation of Fabry Disease

Author(s): Elen Tolstik, Nairveen Ali, Shuxia Guo, Paul Ebersbach, Dorothe Möllmann, Paula Arias-Loza, Johann Dierks, Irina Schuler, Erik Freier, Jörg Debus, Hideo A. Baba, Peter Nordbeck, Thomas Bocklitz and Kristina Lorenz
Published in: International Journal of Molecular Sciences, Issue 23(10), 2022, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23105345

Conference proceedings (8)

Multimodal multiphoton and vibrational microscopy reveals early therapy-induced senescence in human tumors: a non-invasive tool to prevent the risk of cancer relapse

Author(s): Arianna Bresci, Francesco Manetti, Silvia Ghislanzoni, Federico Vernuccio, Salvatore Sorrentino, Chiara Ceconello, Renzo Vanna, Italia Bongarzone, Giulio Cerullo, Dario Polli
Published in: Proc. SPIE, Issue Vol. 12384, 2023
Publisher: SPIE
DOI: 10.1117/12.2648415

Label free highly multimodal nonlinear endoscope using hollow core fiber

Author(s): Hervé Rigneault, Septier, Gaelle Brévalle, Naveen Gajendra, J Saucourt, Géraud Bouwmans, Andy Cassez, Matteo Conforti, Alexandre Kudlinski
Published in:  J. Phys. Chem. B, Issue 127, 16, 2023
Publisher: American Chemical Society

Plug-And-Play Stimulated Raman Microscopy System for Broadband Coherent Vibrational Imaging

Author(s): Francesco Crisafi, Benedetta Talone, Andrea Ragni, Gabriele Di Noia
Published in: (CLEO/Europe-EQEC) 2023 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference, 2023
Publisher: European Physical Society
DOI: 10.1109/cleo/europe-eqec57999.2023.10231815

Video-rate wide-field broadband coherent anti-Stokes Raman scattering microscopy

Author(s): Chiara Ceconello, Federico Vernuccio, Arianna Bresci, Francesco Manetti, Salvatore Sorrentino, Renzo Vanna, Giulio Cerullo, Dario Polli
Published in: Proc. SPIE, Issue Vol. 12390, 2023
Publisher: SPIE
DOI: 10.1117/12.2650628

Ultra-compact widely tunable dual-wavelength fiber-based sources for CARS and SRS imaging (Conference Presentation)

Author(s): Armin Hoffmann; Florian Just; Christian Gaida; Sven Breitkopf; Oliver Herrfurth; Tobias Meyer-Zedler; Jürgen Popp; Tino Eidam; Jens Limpert
Published in: Proc. SPIE, Issue Vol. PC12391, 2023
Publisher: SPIE
DOI: 10.1117/12.2649987

Quantitative molecular absorption of cosmetic active molecules in human skin using coherent Raman imaging (CARS and SRS)

Author(s): Hervé Rigneault, Barbara Sarri
Published in: Conference Papers, Photonics West, 2023
Publisher: Photonics West

Multimodal vibrational and multiphoton nonlinear optical microscopy as a non-invasive tool to prevent human tumor recurrence

Author(s): Arianna Bresci, Francesco Manetti, Silvia Ghislanzoni, Federico Vernuccio, Salvatore Sorrentino, Chiara Ceconello, Renzo Vanna, Italia Bongarzone, Giulio Cerullo, Dario Polli
Published in: Proc. SPIE, Issue Vol. 12627, 2023
Publisher: SPIE
DOI: 10.1117/12.2670918

High-speed vibrational microscopy and deep-learning algorithms to image cells and tissues

Author(s): Dario Polli, Federico Vernuccio, Alejandro De la Cadena, Arianna Bresci, Chiara Ceconello, Francesco Manetti, Salvatore Sorrentino, Subir Das, Renzo Vanna, Giulio Cerullo
Published in: Proc. SPIE LASE, Issue Vol. PC12406, 2023
Publisher: SPIE
DOI: 10.1117/12.2648736

Non-peer reviewed articles (1)

Live stimulated Raman histology for the near-instant assessment of central nervous system samples

Author(s): Barbara Sarri; Sandro Heuke; Chang Liu; Sebastien Boissonneau; Hervé Rigneault; Romain Appay
Published in: Proc. SPIE, Issue Vol. PC12373, 2023, ISSN 1520-6106
Publisher: American Chemical Society
DOI: 10.1117/12.2648086