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NANO-scale VIsualization to understand Bacterial virulence and invasiveness - based on fluorescence NANOscopy and VIBrational microscopy

Objective

In an interdisciplinary project, we will prototype a next-generation super-resolution microscope (SRM) and demonstrate its capability to bring about a major leap forward in our understanding of inter- and intracellular processes, and thus the cellular origin of diseases. Based on the recently invented MINFLUX concept, which pushes spatial resolution an order of magnitude beyond any other SRM technique, and by concerted development of detector technologies, lasers and image acquisition procedures, we will be able to retrieve information, not within reach by any other photonics-based technique. By extending operation to the near infrared, a hitherto un-accessible spectral range for SRM, we will strongly reduce phototoxicity and scattering, increase penetration depth and provide an additional spectral window for multiplexing. The developed prototype will allow nanometer-scale protein localization patterns to be resolved and to be placed in a cellular context by overlaid morphological, biochemical and metabolic images generated by label-free stimulated Raman scattering (SRS) and two-photon excitation (TPE).
In a lead application, we will use the unique capabilities of the to-be-developed technology to study the molecular mechanisms underlying pneumococcal disease, largely attributed to localization patterns of specific bacterial surface proteins, and their intricate interactions with immune and host target cells. Pneumococci are a major contributor to morbidity and mortality worldwide and we aim to provide vital information which can lead to new treatments and vaccines. We will also offer hands-on access to the technology to researchers from both academia and industry in an open demonstration facility. Together with the lead application, this will generate demand for microscopes, lasers and detectors, which the industrial partners will develop subsequent to this project based on the prototypes, further strengthening Europe´s industrial position in the microscopy field.

Call for proposal

H2020-ICT-2020-2
See other projects for this call

Funding Scheme

RIA - Research and Innovation action

Coordinator

KUNGLIGA TEKNISKA HOEGSKOLAN
Address
Brinellvagen 8
100 44 Stockholm
Sweden
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 135 500

Participants (5)

KAROLINSKA INSTITUTET
Sweden
EU contribution
€ 652 600
Address
Nobels Vag 5
17177 Stockholm
Activity type
Higher or Secondary Education Establishments
ABBERIOR INSTRUMENTS GMBH
Germany
EU contribution
€ 1 552 873
Address
Hans Adolf Krebs Weg 1
37077 Gottingen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
INSTITUT FUR NANOPHOTONIK GOTTINGEN EV
Germany
EU contribution
€ 922 156
Address
Hans Adolf Krebs Weg 1
37077 Gottingen
Activity type
Research Organisations
APE ANGEWANDTE PHYSIK UND ELEKTRONIK GMBH
Germany
EU contribution
€ 703 025
Address
Plauener Strasse 163 165
13053 Berlin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
PI IMAGING TECHNOLOGY SA
Switzerland
EU contribution
€ 669 375
Address
Rue De La Pierre A Mazel 39
2000 Neuchatel
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)