Description du projet
Prédire la dynamique des enzymes
Le domaine de la biotechnologie industrielle passe progressivement de la catalyse, reposant uniquement sur la chimie, à l’utilisation d’enzymes pour induire des réactions clés. Des facteurs tels que la réduction des coûts, le respect de l’environnement, l’efficacité et la spécificité font de la biocatalyse une solution alternative attrayante. Le projet PREDACTED, financé par l’UE, vise à comprendre comment les enzymes fonctionnent, s’adaptent à différentes conditions environnementales et évoluent. Les chercheurs développeront des modèles de simulation capables de prédire la dynamique des enzymes et les changements associés à la catalyse. Les résultats obtenus permettront de concevoir des enzymes artificielles améliorées et de relever les principaux défis liés à leur utilisation, tels que la résistance aux antibiotiques.
Objectif
Enzymes are superlative catalysts, honed by billions of years of evolution to achieve high specificity and efficiency. Understanding how enzymes function and are adapted to different environments will be essential in developing biocatalysts for the circular economy and in combating drug resistance. Basic principles of catalysis and mechanistic understanding have come from experiments and simulations, and significant progress has been made in designing and evolving de novo protein catalysts. Truly predictive understanding is limited, however. There is a need for models able to predict, e.g. whether an enzyme is able to break down a particular antibiotic and how to inhibit it; temperature dependence of catalysis; how mutations affect activity and temperature optima; to understand how catalytic power evolves and use those principles in the development of new biocatalysts.
Theoretical developments, and emerging multiscale methods, provide a route to the predictive understanding required. Fundamentally, protein dynamics are vital, not in ‘driving’ reaction but rather as a fundamental facet of natural enzymes on which evolution acts. PREDACTED will simulate enzyme dynamics and dynamical changes associated with catalysis. We will (1) Investigate the adaptation of enzyme activity using the emerging theoretical framework of macromolecular rate theory, and develop simulation approaches to predict enzyme temperature optima, with relevance e.g. for understanding ecosystem response to climate change, and for the development of biocatalysts for practical industrial applications. (2) Develop predictive simulation models for enzymes responsible for antibiotic resistance, analyse allosteric effects and predict spectrums of activity. (3) Model antibiotic breakdown and inhibition of beta-lactamases (4) Apply the understanding developed in redesigning and engineering natural and artificial enzymes to test the catalytic principles and demonstrate how they can be applied in practice.
Champ scientifique
- medical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantibiotics
- natural scienceschemical sciencescatalysisbiocatalysis
- natural sciencesearth and related environmental sciencesatmospheric sciencesclimatologyclimatic changes
- medical and health sciencesbasic medicinepharmacology and pharmacydrug resistanceantibiotic resistance
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
Programme(s)
Thème(s)
Régime de financement
ERC-ADG - Advanced GrantInstitution d’accueil
BS8 1QU Bristol
Royaume-Uni