Descrizione del progetto
Prevedere la dinamica enzimatica
Il campo della biotecnologia industriale si sta spostando dalla catalisi basata solo sulla chimica all’uso di enzimi per catalizzare le reazioni chiave. Fattori come la riduzione dei costi, l’ecocompatibilità, l’efficacia e la specificità rendono la biocatalisi un’intrigante soluzione alternativa. Il progetto PREDACTED, finanziato dall’UE, è interessato a capire come gli enzimi funzionano, si adattano a diverse condizioni ambientali e si evolvono. I ricercatori svilupperanno modelli di simulazione che possono prevedere le dinamiche degli enzimi e i cambiamenti associati alla catalisi. I risultati contribuiranno a progettare enzimi artificiali migliori e ad affrontare le sfide cruciali associate all’uso degli enzimi, come la resistenza agli antibiotici.
Obiettivo
Enzymes are superlative catalysts, honed by billions of years of evolution to achieve high specificity and efficiency. Understanding how enzymes function and are adapted to different environments will be essential in developing biocatalysts for the circular economy and in combating drug resistance. Basic principles of catalysis and mechanistic understanding have come from experiments and simulations, and significant progress has been made in designing and evolving de novo protein catalysts. Truly predictive understanding is limited, however. There is a need for models able to predict, e.g. whether an enzyme is able to break down a particular antibiotic and how to inhibit it; temperature dependence of catalysis; how mutations affect activity and temperature optima; to understand how catalytic power evolves and use those principles in the development of new biocatalysts.
Theoretical developments, and emerging multiscale methods, provide a route to the predictive understanding required. Fundamentally, protein dynamics are vital, not in ‘driving’ reaction but rather as a fundamental facet of natural enzymes on which evolution acts. PREDACTED will simulate enzyme dynamics and dynamical changes associated with catalysis. We will (1) Investigate the adaptation of enzyme activity using the emerging theoretical framework of macromolecular rate theory, and develop simulation approaches to predict enzyme temperature optima, with relevance e.g. for understanding ecosystem response to climate change, and for the development of biocatalysts for practical industrial applications. (2) Develop predictive simulation models for enzymes responsible for antibiotic resistance, analyse allosteric effects and predict spectrums of activity. (3) Model antibiotic breakdown and inhibition of beta-lactamases (4) Apply the understanding developed in redesigning and engineering natural and artificial enzymes to test the catalytic principles and demonstrate how they can be applied in practice.
Campo scientifico
- medical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantibiotics
- natural scienceschemical sciencescatalysisbiocatalysis
- natural sciencesearth and related environmental sciencesatmospheric sciencesclimatologyclimatic changes
- medical and health sciencesbasic medicinepharmacology and pharmacydrug resistanceantibiotic resistance
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
Programma(i)
Argomento(i)
Meccanismo di finanziamento
ERC-ADG - Advanced GrantIstituzione ospitante
BS8 1QU Bristol
Regno Unito