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Multicolor Fluorescent Ultrashort Carbon Nanotubes for Multiplexed immunofluorescence in Cancer Diagnostics

Project description

Nanomaterials to replace antibodies in cancer screening

Histo-pathology is the tool of choice for reliable diagnosis of many cancers. In most cases, pathologists also need to test a high number of biomarkers on a given tumour for better diagnostic and adapted treatments. Today, biomarker analyses are sequential and require several biopsy slices implying not always available biological material. Testing several biomarkers in an efficient, quantitative and reproducible way on a single tissue biopsy slice, is key. To address this technical challenge, the EU-funded Multi-IF project aims to generate novel fluorescent biomarkers based on carbon nanotubes. Researchers will also develop a new microscope capable of detecting multiplex immunofluorescence on a single biopsy. Project deliverables will significantly advance existing cancer diagnosis approaches.

Objective

Multi-IF proposes a fully innovative approach combining nanoscience, fluorescence microscopy and cancer histopathology to achieve optically multiplexed immunofluorescence for cancer diagnostic. At present, when examining cancer tissue, biomarker analyses are sequential and require several biopsy slices implying a lot of (not always available) biological material. Testing several biomarkers on a single tissue biopsy slice in an efficient, quantitative and reproducible way, would be crucial but not currently available at hospital. This project will involve an innovative technology based on single-walled carbon nanotubes to create a library of novel multicolor immuno-labels. More precisely, this will employ sp3 defects implanted fluorescent ultrashort nanotubes together with the development of a dedicated NIR microscope for multiplexed immunofluorescence on a single biopsy and the proof-of-concept on liver cancer tissue. The applicant will bring strong expertise in chemical synthesis, bioprobe spectroscopy and time-resolved fluorescence microscopy applied to the study of complex and heterogeneous bio-molecular organization (2D/3D cells, proteins and peptides). Concomitantly, he will gain a high-level hands-on training on carbon nanotube optics, near-infrared advanced spectroscopy, custom-built microscopy techniques, cancer tissue model and imaging analysis. To achieve this objective, the project will be performed at one of the leading French research institution, IOGS-LP2N at the CNRS/Univ. Bordeaux, with well-identified work packages to ensure efficient management, career development and training, science development, communication and technology transfer to pathologists. It will allow the applicant to reach high level of professional maturity to develop independent research career. The fellow’s expertise, privileged environment of the hosting lab, the mentoring, the infrastructure & relevant facilities will guarantee the successful execution of Multi-IF goals.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2020

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Coordinator

INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE IOTA - SUPOPTIQUE
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 184 707,84
Address
AVENUE AUGUSTIN FRESNEL - CAMPUS POLYTECHNIQUE - RD 128 2
91120 Palaiseau
France

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Region
Ile-de-France Ile-de-France Essonne
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 184 707,84
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