Project description
Sulfur metabolism in yeast
Hydrogen sulfide (H2S) is a gaseous molecule that serves as a signaling mediator in the maintenance of cellular homeostasis, limiting damage to tissues. Despite evidence suggesting its beneficial impact on the mammalian gut microbiome, little is known about the role of H2S in microbiology. The EU funded Yeast H2S Signalling project will investigate the sulfur metabolism pathway in Saccharomyces cerevisiae and delineate how yeast responds to H2S. The work will uncover how this metabolic pathway influences cell-cell interactions and fitness and generate quantitative tools for quantifying volatiles and their impact on microbial interactions.
Objective
Gaseous mediators are ubiquitous in biology. For example, hydrogen sulfide (H2S) signalling leads to cardioprotective effects in humans and is believed to be beneficial for the mammalian gut microbiome. However, gaseous mediators in microbiology are under-explored because quantification of volatiles is challenging. Here, I will investigate how the volatile metabolite H2S can mediate cell-cell interactions and trigger a hitherto unknown sulfur metabolism pathway in Saccharomyces cerevisiae. Conventionally, Met17 is believed to be the only enzyme that can assimilate H2S (generated from inorganic sulfate) into organic compounds, and hence essential for growth on inorganic sulfur sources. However, I observed that the met17 deletion mutant can in fact grow on inorganic sulfate, albeit only at sufficiently high initial cell densities. My preliminary analyses suggest that H2S accumulates in met17- cultures, and when it reaches a threshold, enables cell growth by triggering an alternative sulfur metabolism pathway. In this proposal, I will 1) quantitatively understand how the alternative pathway responds to H2S by developing a mathematical model of density-dependent growth in met17- populations; 2) uncover the novel sulfur metabolism pathway in yeast by using transcriptomics and genetics; and 3) investigate the contribution of this H2S-responsive pathway to the fitness of wildtype yeast using population dynamics analyses. My research will not only offer mechanistic insights into how hidden aspects of yeast sulfur metabolism may contribute to cell-cell interactions and fitness, but also provide quantitative tools for studying gas-mediated microbial interactions from an interdisciplinary perspective. Finally, the project will extensively train me in the synergistic interplay between experiments and mathematical modelling — a style I hope to establish in my future, independent research.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics
- natural sciences chemical sciences organic chemistry
- natural sciences biological sciences microbiology
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences mathematics applied mathematics mathematical model
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2020
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
WC1E 6BT LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.