Project description
Mechanism behind plant response to ambient light
Only limited knowledge currently exists on how polycomb repressive complex 2 (PRC2) participates in the operational control in plants. Following recent observations of altered phenotypes of PRC2-depletion mutants grown under different light intensities, researchers have hypothesised that PRC2 acts in fully differentiated cells to modulate the ambient light acclimation. The EU-funded RPAAL project will identify short- and long-term light acclimation responses that require PRC2 activity to determine PRC2-governed molecular mechanisms and PRC2-modulated light signalling pathways. RNA-seq and ChIP-seq molecular tools will be employed to characterise transcriptome and H3K27me3 distribution and characterise how the epigenome and transcriptome are altered. The project's focus will then be extended to include other chromatin modification mutants displaying altered light acclimation responses.
Objective
Polycomb Repressive Complex 2 (PRC2) is an evolutionarily conserved histone methyltransferase complex that catalyses trimethylation of lysine 27 of histone H3 (H3K27me3), which leads to epigenetic gene repression. In Arabidopsis thaliana (A. thaliana), CURLY LEAF (CLF) and SWINGER (SWN) are two major H3K27 methyltransferases and core components of PRC2 in the sporophyte, playing essential roles in the regulation of plant development. However, the knowledge of how PRC2 participates in operational control in plants is scarce. Recently, altered phenotypes of PRC2-depletion mutants grown under different light intensities were observed. Hence, here I raise the hypothesis that PRC2 acts in fully differentiated cells to modulate the ambient light acclimation. At first, short- and long-term light acclimation responses that require PRC2 activity will be identified, employing plant morphology and physiology tools. Next, PRC2-governed molecular mechanisms and PRC2-modulated light signaling pathway in the light acclimation will be established. Molecular tools (RNA-seq and ChIP-seq) will be used to profile transcriptome and H3K27me3 distribution to establish the patterns of epigenome and transcriptome alteration in WT and mutants. At last, the focus will expand to select other chromatin modification mutants displaying altered light acclimation responses. In summary, for the first time, dynamics of PRC2 repression in fully differentiated cells in response to light acclimation will be determined and it will lay a solid foundation for studying other chromatin modifiers in future. The project builds on combining my experience in plant physiology and ecology and gaining research experience in the field of developmental epigenetics. The fellowship proposal is designed in a way to enhance my independent thinking capacity, organizational and management skills and responsibilities, which are necessary to become an excellent and independent researcher and group leader in the future.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences ecology
- medical and health sciences basic medicine physiology
- natural sciences biological sciences genetics epigenetics
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2020
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
370 05 Ceske Budejovice
Czechia
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.