The researcher and the host team worked towards learning new skills and state-of-the-art techniques to expand knowledge on DNA methylation. Specifically, the action deeply explored the gene dosage compensation in a female heterogametic sex determination system (such as birds, contrary to male heterogametic systems, such as mammals, humans included) by DNA methylation and the distribution of age-related DNA methylation sites across avian genomes. To make sure the pattern held in more than one bird species, the jackdaw data was complemented with data form the zebra finch (Taeniopygia guttata). Practical know-how on techniques for analyzing DNA methylation, like whole genome bisulfite sequencing, has been gained. Participation in sample extraction and shipment to the sequencing facility was also part of the action. This was followed by engaging analytical work, including processing of large datasets using high-performance computing clusters. The creation of a complete pipeline for analyzing DNA methylation data was another significant achievement. As a result, two publications are currently being finalized for submission. The first manuscript shows a notable decrease in DNA methylation with chromosome length. Unique methylomes of sex chromosomes were observed, with higher methylation on the W chromosome compared to all other chromosomes in both species. Additionally, the study revealed elevated methylation on the Z chromosome relative to autosomes in zebra finches. The higher hypomethylation in gene promoters on male Z compared to female Z implies that upregulation on gene-by-gene basis across female Z chromosome is not occurring. The second manuscript focuses on identifying age-related changes in DNA methylation. It reveals disproportionate representation of age-related CpG sites on sex chromosomes in both species. Furthermore, involvement in a review paper on DNA methylation markers of aging in non-model animals was part of the action, resulting in a publication:
https://zenodo.org/records/10914373(s’ouvre dans une nouvelle fenêtre). The fellowship also involved hands-on work with jackdaws in the field, providing valuable insights into the ecological context of the research. Attendance in various courses further enhanced transferable skills, including aspects of academic leadership, PhD supervision, active bystander and media training. Dissemination of research findings to the public and scientific community through educational workshops and conferences was also carried out. Additionally, involvement in supervisory activities and community engagement, such as serving as a postdoc council member, was part of the experience.
