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Area-specific transcriptional dynamics and plasticity of neocortical neurons

Descripción del proyecto

Dilucidar la plasticidad neuronal durante el desarrollo encefálico

El desarrollo encefálico está guiado por señales externas y procesos celulares inherentes que dirigen un circuito de neuronas diversas. Esto ocurre tanto antes como después del nacimiento. Para revelar la función encefálica, es necesario comprender cómo las señales externas interactúan y se integran con las vías internas para producir la identidad neuronal. El proyecto ArealPlasticity, financiado con fondos europeos, empleará un modelo murino para estudiar la plasticidad neuronal mediante el trasplante para imitar el cambio en el entorno externo. Sus investigadores tienen como objetivo identificar los genes implicados en la plasticidad neuronal desencadenada por el entorno durante la diferenciación. Los resultados tendrán repercusiones importantes para los trastornos psiquiátricos y del neurodesarrollo.

Objetivo

Cellular diversity in the nervous system determines the variety of circuits that set the framework for brain function. These different types of neurons emerge during pre- and post-natal development through the regulation of gene networks by two archetypical processes: cell-intrinsic processes, which are independent of environmental conditions, and cell-extrinsic processes, which are triggered by environmental signals. A continuum of interactions between intrinsic and extrinsic processes underlie cellular states. However, their respective contribution to neuronal identity has been difficult to untangle because neurons are highly interconnected and heterogeneous cell-types with distinct and dynamic sensitivities to environmental signals. Here, using the mouse neocortex as a model system, I will investigate how cell-intrinsic and cell-extrinsic processes interact to define neuronal identities using Patch-seq assessment of neuronal molecular identity following transplantation across cortical areas. Neuronal “plasticity” will be assessed by transplantation, which corresponds to the artificial altering of the environmental factors. Data comparison between transplanted neurons and controls will identify the candidate of “core genes” which regulate the environment-dependent plasticity of neuronal differentiation. Finally, I will manipulate these candidate genes and analyze their effect on final neuronal identity to validate their causal relationship. Altogether, this study will contribute to revealing the plasticity of neuronal identity across cortical areas and to addressing environment-dependent molecular mechanisms controlling the plasticity. In the long term, this may contribute to a better understanding of neurodevelopmental and psychiatric disorders, in which cell-intrinsic and extrinsic factors interact to produce the disease.

Ámbito científico (EuroSciVoc)

CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural.

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Coordinador

UNIVERSITE DE GENEVE
Aportación neta de la UEn
€ 203 149,44
Dirección
RUE DU GENERAL DUFOUR 24
1211 Geneve
Suiza

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Región
Schweiz/Suisse/Svizzera Région lémanique Genève
Tipo de actividad
Higher or Secondary Education Establishments
Enlaces
Coste total
€ 203 149,44