Gaining insights into Enzymes Transition States: Superacid as new tool to design specific CAZymes inhibitors.

Description du projet

Combiner la chimie des superacides et la glycoscience pour accélérer la découverte de médicaments

Les glucides sont des macromolécules complexes impliqués dans de nombreux processus biologiques qui ont besoin d’un large éventail d’enzymes actives sur les glucides pour leur synthèse et leur dégradation. Toutefois, le manque d’inhibiteurs à haute sélectivité qui modulent leur activité entrave la découverte de nouveaux agents thérapeutiques sûrs et efficaces. Financé par le programme Actions Marie Skłodowska-Curie, le projet GLYCOMIMIC utilisera la chimie des superacides pour renforcer les connaissances sur les états de transition des enzymes actives sur les glucides et contribuer à la conception d’inhibiteurs efficaces de ces enzymes.

Objectif

Glycosciences study the structural and functional roles of carbohydrates in biology and have an increasing impact on many areas of our economy (health, food, natural resources). Carbohydrate Active enZymes (CAZymes), which catalyse the assembly (Glycosyl transferases, GTs) and breakdown (Glycosidases, GHs) of glycosidic linkage in carbohydrates, are essential to life, being involved in key cell functions. Their pharmacological modulation by means of selective CAZymes’ inhibitors can have multiple applications (e.g. antiviral TamifluTM, antidiabetic GlysetTM, molecular chaperone GalafoldTM). However, the design of inhibitors endowed of high selectivity is challenging, representing the major obstacle in discovering new potent and safe therapeutic agents targeting CAZymes. In this context, GLYCOMIMIC offers an innovative approach based on superacid chemistry to gain insights into CAZymes’ transition states (TS) and to help the design of specific inhibitors, paving the way for short-term impact on drug discovery! Superacid chemistry is only mastered by very few research groups in the world and is a European specificity of the Institute of Chemistry of Poitiers (IC2MP - UMR CNRS 7285 – France) and will be applied for the first time to glycosciences. With Univ. Barcelona (world leader in computer simulation of biological processes at atomic-electronic detail) acting as secondment, GLYCOMIMIC blends multidisciplinary states of the art research training in carbohydrate chemistry, superacids, low-temperature NMR analysis, DFT calculations, QM/MM dynamic simulation of enzymes and enzyme inhibition studies. It will provide a prolific fellow with an internationally competitive training, with the aim of advancing the career of an autonomous scientist in the EU and the next generation of multidisciplinary researchers in (bio)pharmaceutical sciences.