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High-throughput droplet-based single-cell small RNA sequencing technology

Project description

New technology for sequencing non-coding RNAs

RNA sequencing is a technique that allows the quantitative analysis of messenger RNA molecules in a biological sample. However, it is restricted to the protein-coding RNAs and cannot provide the sequence of other RNA molecules such as small non-coding RNAs. The EU-funded droplet-small-seq project aims to address this limitation and offer insight into the expression and function of microRNAs in individual cells. The team will develop a high-throughput droplet-based technology for capturing and sequencing microRNAs and mRNAs at the same time. Given the regulatory role of the non-coding RNAs in gene expression, it is expected that the project's approach will find many useful applications in research.

Objective

Droplet-based single-cell RNA-sequencing (scRNA-seq) technologies have penetrated almost all branches of life sciences and have significantly advanced our understanding of cellular processes and organism development. However, despite their astonishing impact, most of the scRNA-seq technologies reported to date rely on poly(A) tail capture and thus are mainly restricted to the protein-coding RNAs, while neglecting a substantial proportion of the transcriptome, including small non-coding RNAs. As a result, very little is known about the non-coding RNA expression and function in individual cells, and especially their role in the establishment of cellular phenotypic diversity. Small RNAs contain a variety of classes, of which miRNAs are the most common and these act as regulatory molecules by suppressing translation of mRNAs. In addition, loss-of-function studies of miRNAs uncovered their involvement in development of nearly all tissues, including hematopoiesis. However, most studies exploring miRNA dynamics reported to date relied on bulk cell assays, thus disregarding the individual cell types and their heterogeneity. In the scope of this proposal, we aim to develop a high-throughput droplet-based single-cell small RNA-seq (droplet-small-seq) for simultaneous miRNA and mRNA capture and sequencing. We will apply this newly developed technique to investigate the regulatory roles of miRNAs in cell fate decision during hematopoietic development at a single-cell level.

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Topic(s)

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Funding Scheme

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2020

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Coordinator

VILNIAUS UNIVERSITETAS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 146 112,00
Address
UNIVERSITETO G. 3
01513 Vilnius
Lithuania

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Region
Lietuva Sostinės regionas Vilniaus apskritis
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 146 112,00
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