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Interactions between chromatin and transposable elements in rapid adaptation to environmental stress

Project description

How fruit flies adapt to environmental perturbations

Environmentally sensitive interactions between the epigenome and transposable elements (TEs) are underpinned by dynamic chromatin states regulating gene expression. Understanding how chromatin varies across populations and how responsive it is to environmental changes is key to evaluating the role of epigenome-TE interactions in adaptation. The EU-funded InterChromaTE project will measure natural variation in chromatin accessibility in different populations of common fruit flies (D. melanogaster) and identify associations between chromatin accessibility and polymorphic TEs. Proximate mechanisms (RNA and chromatin responses to stress) and phenotypic outcomes (life history traits) will also be measured over multiple generations. Longer-term evolutionary consequences will be measured in invasive and native populations of D. melanogaster and the recent invasive pest species D. suzukii.

Objective

Understanding how organisms adapt to the environmental perturbations, including those caused by human activity, is a key question in evolutionary biology. Some organisms appear to adapt more rapidly than expected, and two factors predicted to contribute to this are environmentally sensitive epigenome regulation and transposable element (TE) activity under stress. Recent molecular studies have shown how interactions between the epigenome and TEs can affect genome evolution in the fruitfly Drosophila melanogaster. However, little is known about how these processes interact in natural populations, limiting our understanding of their role in population adaptation under environmental change.

Environmentally sensitive interactions between the epigenome and TEs are underpinned by dynamic chromatin states regulating gene expression. Understanding how chromatin varies across populations and how responsive it is to environmental change is key to evaluating the role of epigenome-TE interactions in adaptation. Here we will measure natural variation in chromatin accessibility in different populations of D. melanogaster and look for associations between chromatin accessibility and polymorphic TEs. Proximate mechanisms (RNA and chromatin responses to stress) and phenotypic outcomes (life history traits) will be measured over multiple generations. Longer term evolutionary consequences will be measured in invasive and native populations of D. melanogaster and the more recently invading D. suzukii, and TEs associated with differential chromatin regulation in invasive populations will be validated using functional genomics techniques including CRISPR-Cas9.

These data will reveal how interactions between chromatin states and TEs can shape adaptation over different organisational scales, from the molecular to the population-level, and across different temporal scales, including within-generation acclimation, transgenerational epigenetic change and long-term evolutionary change.

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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(opens in new window) H2020-MSCA-IF-2020

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Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 160 932,48
Address
CALLE SERRANO 117
28006 MADRID
Spain

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Comunidad de Madrid Comunidad de Madrid Madrid
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Total cost

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€ 160 932,48
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