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Evaluating effects of rumen originated lipopolysaccharide on the pathogenesis of subacute rumen acidosis

Project description

Deciphering the pathogenesis of subacute ruminal acidosis

Subacute ruminal acidosis (SARA) is a digestive disorder of dairy cows that negatively impacts animal health and industry profitability in general. It is associated with increased ruminal lipopolysaccharide concentration, and preliminary data showed that lipopolysaccharides participate in the pathogenesis of SARA via an increase in the starch-digesting and lactate-producing ruminal bacteria as well as the upregulation of the genes involved in lipopolysaccharide biosynthesis. The EU-funded LIS project’s objectives are to investigate the role of lipopolysaccharides in SARA pathogenesis in detail and provide the direction for the development of the potential products for their inactivation in the rumen.

Objective

Subacute ruminal acidosis (SARA) is a well-recognized digestive disorder of high yielding dairy cows that has a negative impact on both animal health and herd profitability. It is associated with increased ruminal lipopolysaccharide (LPS), and LPS has been reported to participate in the pathogenesis of SARA. LPS from E. coli (LPS-E) has been used to study its effects on the SARA pathogenesis. Our preliminary data showed that LPS-E participates in the pathogenesis of SARA by directly increasing the starch-digesting and lactate-producing ruminal bacteria and the genes involved in the LPS biosynthesis process. However, we do not know if LPS-E would be representative of LPS from ruminal bacteria (LPS-R), and thus we do not know how LPS released from SARA cows participate in the pathogenesis of SARA. Therefore, the objectives of this proposal are to 1) isolate LPS from the rumen bacteria by inducing SARA nutritional model in batch culture (WP1, 2, 5, 6 & 7); 2) and compare the effects of LPS-R with the LPS-E on ruminal fermentation, bacterial community composition (BCC), and function by using 16S rRNA sequencing and RNA sequencing in batch culture to elucidate the roles of LPS on the pathogenesis of SARA (WP1, 3, 4, 5, 6 & 7). We hypothesis that 1) LPS from ruminal bacteria could be collected from culture media through inducing SARA nutritional model in batch culture; 2) LPS-R and LPS-E will have different effects on ruminal fermentation, BCC, and functions, but both of the two LPS sources would stimulate the bacteria and genes involved in starch digestion, lactic acid production, and LPS biosynthesis. We expect that our proposal could 1) fulfill the gap of knowledge of the role of LPS on the SARA pathogenesis; 2) provide the direction for industries to develop the products (e.g. clay) could inactive LPS in the rumen; 3) help European dairy industries save at least 994.4 million euros per year due to lower SARA prevalence.

Coordinator

UNIVERSITA DEGLI STUDI DI MILANO
Net EU contribution
€ 128 604,96
Address
Via Festa Del Perdono 7
20122 Milano
Italy

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Region
Nord-Ovest Lombardia Milano
Activity type
Higher or Secondary Education Establishments
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Total cost
€ 128 604,96