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Evaluating effects of rumen originated lipopolysaccharide on the pathogenesis of subacute rumen acidosis

Description du projet

Décrypter la pathogenèse de l’acidose ruminale subaiguë

L’acidose ruminale subaiguë (ou SARA, pour Subacute ruminal acidosis), un trouble digestif qui touche les vaches laitières, affecte à la fois la santé animale et la rentabilité de l’industrie en général. Elle est associée à une augmentation de la concentration de lipopolysaccharides dans le rumen. Des données préliminaires ont montré que les lipopolysaccharides participent à la pathogenèse de la SARA par le biais d’une augmentation des bactéries ruminales digérant l’amidon et produisant du lactate, ainsi que par la régulation positive des gènes impliqués dans la biosynthèse des lipopolysaccharides. Les objectifs du projet LIS, financé par l’UE, consistent à étudier en détail le rôle des lipopolysaccharides dans la pathogenèse de la SARA et de fournir une orientation pour le développement de produits potentiels susceptibles de permettre leur inactivation dans le rumen.

Objectif

Subacute ruminal acidosis (SARA) is a well-recognized digestive disorder of high yielding dairy cows that has a negative impact on both animal health and herd profitability. It is associated with increased ruminal lipopolysaccharide (LPS), and LPS has been reported to participate in the pathogenesis of SARA. LPS from E. coli (LPS-E) has been used to study its effects on the SARA pathogenesis. Our preliminary data showed that LPS-E participates in the pathogenesis of SARA by directly increasing the starch-digesting and lactate-producing ruminal bacteria and the genes involved in the LPS biosynthesis process. However, we do not know if LPS-E would be representative of LPS from ruminal bacteria (LPS-R), and thus we do not know how LPS released from SARA cows participate in the pathogenesis of SARA. Therefore, the objectives of this proposal are to 1) isolate LPS from the rumen bacteria by inducing SARA nutritional model in batch culture (WP1, 2, 5, 6 & 7); 2) and compare the effects of LPS-R with the LPS-E on ruminal fermentation, bacterial community composition (BCC), and function by using 16S rRNA sequencing and RNA sequencing in batch culture to elucidate the roles of LPS on the pathogenesis of SARA (WP1, 3, 4, 5, 6 & 7). We hypothesis that 1) LPS from ruminal bacteria could be collected from culture media through inducing SARA nutritional model in batch culture; 2) LPS-R and LPS-E will have different effects on ruminal fermentation, BCC, and functions, but both of the two LPS sources would stimulate the bacteria and genes involved in starch digestion, lactic acid production, and LPS biosynthesis. We expect that our proposal could 1) fulfill the gap of knowledge of the role of LPS on the SARA pathogenesis; 2) provide the direction for industries to develop the products (e.g. clay) could inactive LPS in the rumen; 3) help European dairy industries save at least 994.4 million euros per year due to lower SARA prevalence.

Coordinateur

UNIVERSITA DEGLI STUDI DI MILANO
Contribution nette de l'UE
€ 128 604,96
Adresse
Via Festa Del Perdono 7
20122 Milano
Italie

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Région
Nord-Ovest Lombardia Milano
Type d’activité
Higher or Secondary Education Establishments
Liens
Coût total
€ 128 604,96